Rabbit polyclonal antibody raised against synthetic peptide of CCNE2.
Immunogen:
A synthetic peptide corresponding to residues surrounding T392 of human CCNE2.
Host:
Rabbit
Reactivity:
Human, Mouse
Specificity:
This antibody is specific to CCNE2.
Form:
Liquid
Purification:
Affinity purification
Concentration:
1 mg/mL
Recommend Usage:
Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) Immunofluorescence (1:500-1:1000) ELISA (1:5000) The optimal working dilution should be determined by the end user.
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO cells (Lane 1 and lane 3) and Jurkat cells (Lane 2), using CCNE2 polyclonal antibody (Cat # PAB18330). Peptide "+" means "peptide blocking".
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CCNE2 polyclonal antibody (Cat # PAB18330). Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using CCNE2 polyclonal antibody (Cat # PAB18330). Peptide "+" means "peptide blocking".
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq