Rabbit polyclonal antibody raised against synthetic peptide of CCNB1.
Immunogen:
A synthetic peptide corresponding to human CCNB1.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody is specific to CCNB1.
Form:
Liquid
Purification:
Affinity purification
Concentration:
1 mg/mL
Recommend Usage:
Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) Immunofluorescence (1:500-1:1000) ELISA (1:20000) The optimal working dilution should be determined by the end user.
Western blot analysis of extracts from HeLa cells, treated with serum (10 %, 15 min), using CCNB1 polyclonal antibody (Cat # PAB18121). Peptide "+" means "peptide blocking".
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CCNB1 polyclonal antibody (Cat # PAB18121). Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40 nM, 30 mins), using CCNB1 polyclonal antibody (Cat # PAB18121). Peptide "+" means "peptide blocking".
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq