Rabbit polyclonal antibody raised against synthetic peptide of RRAD.
Immunogen:
A synthetic peptide corresponding to internal of human RRAD.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody detects endogenous levels of total RRAD protein.
Form:
Liquid
Recommend Usage:
Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) Immunofluorescence (1:500-1:1000) ELISA (1:20000) The optimal working dilution should be determined by the end user.
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells (Lane 1 and lane 4), HepG2 cells (Lane 2) and MCF-7 cells (Lane 3), using RRAD polyclonal antibody (Cat # PAB17740). Peptide "+" means "with peptide blocking".
Immunohistochemistry analysis of paraffin-embedded human heart tissue using RRAD polyclonal antibody (Cat # PAB17740). Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of A-549 cells, using RRAD polyclonal antibody (Cat # PAB17740). Peptide "+" means "with peptide blocking".