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- Home > Products > Polyclonal Ab > Human > Cell Cycle > MDM2 polyclonal antibody
MDM2 polyclonal antibody
- 目录号 # : PAB1729
- 访问频率 :
- 尺寸
- 价格
- 库存
- 可得性
- 购物车
- 100 ug
- USD $ 319
- order now, ship next day
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MDM2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MDM2.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1.
Burch LR, Scott M, Pohler E, Meek D, Hupp T.J Mol Biol. 2004 Mar 12;337(1):115-28.
- 2.
- Mdm2 mRNA expression in salivary gland tumour cell lines.
Mantesso A, Loducca SV, Bendit I, Garicochea B, Nunes FD, de Araujo VC.J Oral Pathol Med. 2004 Feb;33(2):96-101.
- 3.
- Binding of p53-derived ligands to MDM2 induces a variety of long range conformational changes.
Schon O, Friedler A, Freund S, Fersht AR.J Mol Biol. 2004 Feb 6;336(1):197-202.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of MDM2 polyclonal antibody (Cat # PAB1729) in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Western Blot (Transfected lysate)
- Western blot analysis of MDM2 (arrow) using MDM2 polyclonal antibody (Cat # PAB1729). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MDM2 polyclonal antibody (Cat # PAB1729) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 4193
- Protein Accession#:
- NP_002383;Q00987
- Gene Name:
- MDM2
- Gene Alias:
- HDMX,MGC71221,hdm2
- Gene Description:
- Mdm2 p53 binding protein homolog (mouse)
- Omim ID:
- 164785
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
- Other Designations:
- Mdm2, transformed 3T3 cell double minute 2, p53 binding protein,double minute 2, human homolog of; p53-binding protein,mouse double minute 2 homolog,p53-binding protein MDM2,ubiquitin-protein ligase E3 Mdm2
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