Rabbit polyclonal antibody raised against synthetic phosphopeptide of HDAC1.
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S421/S423 of human HDAC1.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Recommend Usage:
Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) ELISA (1:4000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C for three months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells. Lane 1 : HDAC1 (phospho Ser421/423) polyclonal antibody (Cat # PAB16973). Lane 2 : Using the same antibody preincubated with synthesized peptide.
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq