Rabbit polyclonal antibody raised against synthetic peptide of NTF3.
Immunogen:
A synthetic peptide (conjugated with BSA) corresponding to amino acids 139-149 of human NTF3.
Sequence:
YAEHKSHRGEY
Host:
Rabbit
Reactivity:
Chicken, Human, Rat
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot.
Form:
Lyophilized
Recommend Usage:
Immunohistochemistry (1-10 ug/mL) ELISA (1-10 ug/mL) Western Blot (1-10 ug/mL) Inhibition assay (1-10 ug/mL in vitro 2-10 ug/mL (ED50) in vivo) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS
Storage Instruction:
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Applications
Western Blot
Immunohistochemistry
Immunohistochemical staining of NTF3 in rat purkinje cells of the cerebellum using rabbit NTF3 polyclonal antibody (Cat # PAB0744) .
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq