Mouse monoclonal antibody raised against partial recombinant BCL2.
Recombinant protein corresponding to amino acids 1-211 of human Bcl-2.
Protein G purification
ELISA (1:500) Western Blot (1:250-1:500) Immunofluorescence (1:250-1:500) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.1% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 cell lysates (35 ug) with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution with Texas Red (Red).
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq