Mouse monoclonal antibody raised against partial recombinant TOP2A.
Immunogen:
Recombinant protein corresponding to the C-terminal region of TOP2A.
Host:
Mouse
Reactivity:
Human
Form:
Lyophilized
Isotype:
IgG1
Recommend Usage:
Immunohistochemistry (1:20-1:40) Western Blot (1:250-1:500) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from tissue culture supernatant (15 mM sodium azide)
Storage Instruction:
Store at 4°C on dry atmosphere. After reconstitution with steril distilled water, Store at 4°C for at least two months. For long term storage store at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq
Other Designations:
DNA topoisomerase II, 170 kD,DNA topoisomerase II, alpha isozyme,topoisomerase (DNA) II alpha (170kD)
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