Store at 4°C for three months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Recombinant protein)
Western blot analysis of HSPA5 monoclonal antibody (Cat # MAB8736) at 1 : 2000 dilution interacts with recombinant HSPA5 protein with a DsbA tag. Predicted band size : 48 KDa. Observed band size : 48 KDa.
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM
Other Designations:
Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD),OTTHUMP00000022124,heat shock 70kD protein 5 (glucose-regulated protein, 78kD),heat shock 70kDa protein 5
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