Monocytes of different species and immunoaffinity purified soluble human CD14.
Host:
Mouse
Reactivity:
Bovine, Dog, Horse, Human, Pig
Specificity:
biG 13 represents an excellent marker for CD14. CD14 has been shown to be involved in LPS-induced cell activation. This antigen is present as a membrane bound form and as a soluble antigen, both of which are involved in cell stimulation by LPS. The plasma protein LBP plays an important role in the LPS-CD14 mediated cell activation. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.
Form:
Lyophilized
Isotype:
IgG1
Recommend Usage:
Flow Cytometry (1:5000) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq