Mouse monoclonal antibody raised against partial recombinant FGFR1.
Recombinant protein beginning with proline 23 of human FGFR1.
Reacts with both alpha (denatured) and beta isoforms. Epitope is within the sequence his241 and val267 between Ig loops II and III. Epitope is masked in undenatured FGFr1-alpha. Antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Western Blot (1 ug/mL recognizes 10ng recombinant bacterial baculoviral or native FGFr1) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq