Mouse monoclonal antibody raised against partial recombinant CTCF.
Immunogen:
Recombinant protein corresponding to human CTCF.
Host:
Mouse
Reactivity:
Human, Monkey
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis using CTCF monoclonal antibody, clone 1D11 (Cat # MAB10575) against A31 (1), MCF-7 (2), HeLa (3), HCT116 (4), Jurkat (5), NIH/3T3 (6), and COS-7 (7) cell lysate.
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq