Mouse monoclonal antibody raised against partial recombinant CDH2.
Recombinant protein corresponding to human CDH2.
Theoretical MW (kDa):
Human, Mouse, Rat
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) against A-431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) with DAB staining.
Immunofluorescence analysis of A-431 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of PC-2 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green) and negative control (purple).
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq