Mouse monoclonal antibody raised against partial recombinant CD1A.
Immunogen:
Recombinant protein corresponding to human CD1A.
Host:
Mouse
Theoretical MW (kDa):
37
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis using CD1A monoclonal antibody, clone 7A7 (Cat # MAB10529) against K-562 (1), Raji (2), and MOLT 4 (3) cell lysate.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A), colon cancer tissues (B), brain tissues (C) and submaxillary tumor tissues (D) using CD1A monoclonal antibody, clone 7A7 (Cat # MAB10529) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of Raji cells using CD1A monoclonal antibody, clone 7A7 (Cat # MAB10529) (green). Blue: DRAQ5 fluorescent DNA dye.
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq