Mouse monoclonal antibody raised against recombinant IgG (Fc).
Immunogen:
Fusion protein of human IgG Fc fragment.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
Western Blot (1:500-1:2000) Immunofluorescence (1:200-1:1000) ELISA (1:10001) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascitic (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Recombinant protein)
Western blot analysis using IgG (Fc) monoclonal antibody, clone 10A4 (Cat # MAB10474) against different fusion proteins with human IgG(Fc specific) tag.
Immunofluorescence
Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using IgG (Fc) monoclonal antibody, clone 10A4 (Cat # MAB10474) (green). Blue: DRAQ5 fluorescent DNA dye.