Mouse monoclonal antibody raised against partial recombinant HDAC3.
Recombinant protein corresponding to amino acids 224-428 of human HDAC3.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis using HDAC3 monoclonal antibody, clone 3A7B5 (Cat # MAB10374) against truncated Trx-HDAC3 recombinant protein (1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate (2) and HeLa cell lysate (3).
Immunohistochemical analysis of paraffin-embedded human esophagus cancer (A) and breast carcinoma tissue (B), showing nuclear localization. using HDAC3 monoclonal antibody, clone 3A7B5 (Cat # MAB10374) with DAB staining.