Mouse monoclonal antibody raised against native FCGR3A.
Immunogen:
Native purified FCGR3A from human granulocytes.
Host:
Mouse
Reactivity:
Human, Pig
Specificity:
This antibody reacts with CD16 antigen (isoform CD16a). CD16a is a transmembrane form of CD16, expressed on NK-cells, monocytes and macrophages.
Form:
Liquid
Concentration:
1 mg/mL
Isotype:
IgM
Recommend Usage:
The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (15 mM sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Flow Cytometry
Surface staining of lysed and washed porcine peripheral blood with purified FCGR3A monoclonal antibody, clone MEM - 168 (Cat # MAB0953) (detection by anti - mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control. Panel B, C, D - three different porcine PMBC samples stained with FCGR3A monoclonal antibody, clone MEM - 168 (Cat # MAB0953). Cells in the granulocyte gate were used for analysis.
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations:
Fc fragment of IgG, low affinity III, receptor for (CD16),Fc fragment of IgG, low affinity IIIa, receptor for (CD16),Fc gamma receptor III-A,Fc-gamma RIII-alpha,Fc-gamma RIIIa,Fc-gamma receptor III-2 (CD 16),Fc-gamma receptor IIIb (CD16),FcgammaRIIIA,OTTH
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