Transient overexpression cell lysate was tested with Anti-CDK2AP1 antibody (H00008099-B02) by Western Blots. SDS-PAGE Gel CDK2AP1 transfected lysate. Western Blot Lane 1: CDK2AP1 transfected lysate ( 12.40 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes. [provided by RefSeq
Other Designations:
CDK2-associated protein 1,CDK2-associated protein 1,Deleted in oral cancer-1,cyclin-dependent kinase 2-associated protein 1,putative oral cancer suppressor