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- Home > Products > Monoclonal Ab > Human > Enzyme > Kinase > MAPK8 monoclonal antibody (M11), clone 2F11
MAPK8 monoclonal antibody (M11), clone 2F11
- 目录号 # : H00005599-M11
- 访问频率 :
- 尺寸
- 价格
- 库存
- 可得性
- 购物车
- 100 ug
- USD $ 319
- order now, ship next day
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- 该价格仅在China有效。请选择国家。
- 询问我们任何问题
- 付款信息
信用卡 :
Email : sales@abnova.com
Phone : +886-2-87511888
Fax : +886-2-87511166- 产品会直接装运至客户处。
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant MAPK8.
- Immunogen:
- MAPK8 (NP_002741.1, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLAQVQQ
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.87 KDa) .
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11.
Lane 1: MAPK8 transfected lysate(48.3 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to MAPK8 on HeLa cell . [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged MAPK8 is 1 ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi ( Cat # H00005599-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK8 monoclonal antibody (M11) clone 2F11 (Cat # H00005599-M11 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between CRK and MAPK8 HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between PXN and MAPK8 Huh7 cells were stained with anti-PXN rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between BAD and MAPK8 Mahlavu cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Application Image
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunofluorescence
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- In situ Proximity Ligation Assay (Cell)
- enlarge
- In situ Proximity Ligation Assay (Cell)
- enlarge
- In situ Proximity Ligation Assay (Cell)
- enlarge
- Entrez GeneID:
- 5599
- GeneBank Accession#:
- NM_002750.1
- Protein Accession#:
- NP_002741.1
- Gene Name:
- MAPK8
- Gene Alias:
- JNK,JNK1,JNK1A2,JNK21B1/2,PRKM8,SAPK1
- Gene Description:
- mitogen-activated protein kinase 8
- Omim ID:
- 601158
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
- Other Designations:
- JNK1 alpha protein kinase,JNK1 beta protein kinase,JUN N-terminal kinase,OTTHUMP00000019552,OTTHUMP00000019555,OTTHUMP00000019556,OTTHUMP00000019558,c-Jun N-terminal kinase 1,mitogen-activated protein kinase 8 isoform JNK1 alpha1,mitogen-activated protein
- Interactome
- Gene Pathway
- Adipocytokine signaling pathway
- Colorectal cancer
- Epithelial cell signaling in Helicobacter pylori infection
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Focal adhesion
- GnRH signaling pathway
- Insulin signaling pathway
- MAPK signaling pathway
- Neurotrophin signaling pathway
- Pancreatic cancer
- Pathways in cancer
- Toll-like receptor signaling pathway
- Type II diabetes mellitus
- Wnt signaling pathway
