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- Home > Products > Monoclonal Ab > Human > Transcriptions > PRDM1 monoclonal antibody (M01), clone 2B10
PRDM1 monoclonal antibody (M01), clone 2B10
- 目录号 # : H00000639-M01
- 访问频率 :
- 尺寸
- 价格
- 库存
- 可得性
- 购物车
- 100 ug
- USD $ 319
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- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant PRDM1.
- Immunogen:
- PRDM1 (NP_001189, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MKMDMEDADMTLWTEAEFEEKCTYIVNDHPWDSGADGGTSVQAEASLPRNLLFKYATNSEEVIGVMSKEYIPKGTRFGPLIGEIYTNDTVPKNANRKYFWRIYSRGELH
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- MSDS:
Download
- Applications
- Western Blot (Tissue lysate)
- PRDM1 monoclonal antibody (M01), clone 2B10. Western Blot analysis of PRDM1 expression in human spleen.
- Protocol Download
- Western Blot (Tissue lysate)
- PRDM1 monoclonal antibody (M01), clone 2B10. Western Blot analysis of PRDM1 expression in human tongue.
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of PRDM1 expression in transfected 293T cell line by PRDM1 monoclonal antibody (M01), clone 2B10.
Lane 1: PRDM1 transfected lysate(88 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged PRDM1 is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of PRDM1 over-expressed 293 cell line, cotransfected with PRDM1 Validated Chimera RNAi ( Cat # H00000639-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRDM1 monoclonal antibody (M01), clone 2B10 (Cat # H00000639-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 639
- GeneBank Accession#:
- NM_001198
- Protein Accession#:
- NP_001189
- Gene Name:
- PRDM1
- Gene Alias:
- BLIMP1,MGC118922,MGC118923,MGC118924,MGC118925,PRDI-BF1
- Gene Description:
- PR domain containing 1, with ZNF domain
- Omim ID:
- 603423
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq
- Other Designations:
- B-lymphocyte-induced maturation protein 1,OTTHUMP00000016918,PR-domain zinc finger protein 1,PRDI-binding factor-1,beta-interferon gene positive-regulatory domain I binding factor,positive regulatory domain I-binding factor 1
