by
Abnova
Calcium phosphate transfection method is a simple, time efficient and relatively inexpensive way to introduce DNA into cells in stable cell lines such as HEK293T, CHO and BHK cells. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.
by
Abnova
Captor™
is a label-free, microfiltration system for isolation, enumeration, and retrieval of circulating tumor cells (CTCs). Captor™
system utilizes a proprietary Clear Cell™
CTChip®
technology based on sophisticated fluid dynamics and myriads of crescent-shaped microwells to ensure capturing of CTCs while leaving other whole blood constituents to flow through.The elegant and simplistic design maximizes CTCs isolation efficiency, purity, and function by preserving CTCs surface integrity, allowing retrieval of the viable cells in their native state. This video shows you how to install Captor™
easily.
by
Abnova
Captor™ is a label-free, microfiltration system for isolation, enumeration, and retrieval of circulating tumor cells (CTCs). Captor™ system utilizes a proprietary Clear Cell™ CTChip® technology based on sophisticated fluid dynamics and myriads of crescent-shaped microwells to ensure capturing of CTCs while leaving other whole blood constituents to flow through.The elegant and simplistic design maximizes CTCs isolation efficiency, purity, and function by preserving CTCs surface integrity, allowing retrieval of the viable cells in their native state.
by
Abnova
jetPEI™, a polyethylenimine (PEI) derivative, is a cationic polymer transfection reagent that binds to DNA by electrostatic forces. The cationic polymer/DNA complex enters the cell by endocytosis, where the DNA is then released into the cytoplasm.
by
Abnova
Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.
by
Abnova
The Nexcelom Bioscience Cellometer® is a simple to use, automated cell counter designed to improve accuracy and repeatability. You just pipette 20μL of sample, insert the slide and click count. Cell Concentration, cell size and viability are displayed in less than 30 seconds.
by
Abnova
Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.
by
Abnova
Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.
by
Abnova
Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture attached cells from cell thawing, cell maintenance, to cell freezing.
by
Abnova
Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture suspension cells from cell thawing, cell maintenance, to cell freezing.
by
Abnova
The cells must be fixed and permeabilized to ensure free access of the antibody to its antigen. Fixation methods fall generally into two classes: organic solvents and cross-linking reagents. This video uses formaldehyde as a cross-linking reagent to fix cells.
by
Abnova
Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. This video shows the procedure for using "CoolCell". The "CoolCell" is an alcohol-free freezing container which provides the ideal freezing rate close to minus 1°C/minute required for successful cryopreservation of cells.
by
Abnova
Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. This video shows the procedure for using "Mr. Frosty". The "Mr. Frosty" is a freezing container filled with isopropyl alcohol at room temperature. It provides the idea cooling rate close to minus 1°C/minute required for successful cryopreservation of cells.
by
Abnova
Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. The cryopreservation agent and condition can depend on cell type. This video shows you how to transport cryovials from 4°C, -20°C, -80°C mechanical freezers to liquid nitrogen tank while maintaining optimal cell health.
by
Abnova
Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. This video shows the procedure for cell freezing with programmable freezer. By using programmable freezer, you could control and ensure the freezing rates are exactly as required. It is the most reliable and reproducible way to freeze cells.
by
Abnova
Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. This video shows the procedure for using Styrofoam box. The Styrofoam box slows the rate of freezing, so that ice crystals dont form too fast and destroy the cells.
by
Abnova
This protocol involves three steps to prepare cell lysate. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as read-to-use, denatured lysate in 1x Sample Buffer.
by
Abnova
This protocol involves three steps to prepare cell lysate. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as read-to-use, native lysate in modified RIPA Buffer.
by
Abnova
This protocol involves three steps to prepare nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer.
by
Abnova
CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound (MTS). The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at 490nm.
by
Abnova
The Cell Proliferation Reagent WST-1 is a clear, slightly red, ready-to-use solution, containing WST-1, a tetrazolium salt. By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of 440 nm.
by
Abnova
Cell Synchronization is a process by which cells at different stages of the cell cycle in a culture are brought to the same phase. Nocodazole is one of anticancer drugs that synchronize cells at M-phase. It can interfere with the structure and function of microtubules in interphase and mitotic cells.
by
Abnova
The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath. Working quickly ensures a high survival rate of the cells.
by
Abnova
The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath. Working quickly ensures a high survival rate of the cells. This video describes the thawing procedure for DMSO-sensitive cells.
by
Abnova
Chimera RNAi is a process by which small interfering RNA/DNA chimera triggers the destruction of mRNA. The discovery work, design, and application of chimera RNAi have been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.
by
Abnova
Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate.
by
Abnova
The Fyrite gas analyzer is the most widely used instrument for measuring carbon dioxide (CO2) levels in incubators. It uses the Orsat method of volumetric analysis involving chemical (potassium hydroxide) absorption of CO2 gas.
by
Abnova
Plate surface that is coated with poly-D-lysine possesses a uniform net positive charge. This uniform net positive charge is preferred by certain cell types and can subsequently enhance cell attachment, growth and differentiation.
by
Abnova
Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. Because DNA is hydrophilic, it will not normally pass through membrane. In order to make these cells readily incorporate foreign DNA, they must first be made "competent" to take up foreign DNA.
by
Abnova
In competitive ELISA, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well. The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.
by
Abnova
Cre-Lox recombination is a special type of site-specific recombination. The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. It is often used in the generation of knockout and conditional knockout animals.
by
Abnova
Cytoscape is an open source bioinformatics software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data.
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