Rabbit polyclonal antibody raised against synthetic peptide of CASP3.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to internal region of human CASP3.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Ammonium sulfate precipitation
Recommend Usage:
ELISA (1:1000) Western Blot (1:50-100) Immunohistochemistry (1:10-50) Flow cytometry (1:10-50) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of CASP3 polyclonal antibody (Cat # PAB2069) in NCI-H460 cell line lysates (35 ug/lane).CASP3 (arrow) was detected using the purified Polyclonal antibody (1 : 60 dilution).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 polyclonal antibody (Cat # PAB2069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
ELISA
Flow Cytometry
Flow cytometric analysis of NCI-H460 cells using CASP3 polyclonal antibody (Cat # PAB2069)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
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