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AKT1 polyclonal antibody   

  • 目录号 # : PAB19801
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  • 购物车
  • 100 ug
  • USD $ 319
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  • Email : sales@abnova.com
    Phone : +886-2-87511888
    Fax : +886-2-87511166
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
  • Immunogen:
  • A synthetic peptide corresponding to 15 amino acids at internal region of human AKT1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Peptide affinity purification
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:200-1000)
    Immunohistochemistry (1:100-500)
    Immunofluorescence (1:100-1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (2% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of MCF-7 cell lysate with AKT1 polyclonal antibody (Cat # PAB19801) at 1:200 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of formalin-fixed paraffin-embedded human liver showing staining with AKT1 polyclonal antibody (Cat # PAB19801) at 1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of HeLa with AKT1 polyclonal antibody (Cat # PAB19801) at 1:50 dilution.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 207
  • Gene Name:
  • AKT1
  • Gene Alias:
  • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 1
  • Gene Summary:
  • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
  • Interactome
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