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  • alamarBlueR® Cell Proliferation Indicator

    alamarBlueR works as a cell viability and proliferation indicator through the conversion of resazurin to resorufin. Resazurin, a non-fluorescent indicator dye, is converted to highly red fluorescent resorufin via reduction reactions of metabolically active cells. The amount of fluorescence produced is proportional to the number of living cells.

    Publish: 2010/12/6

  • Alkaline Phosphatase Staining

    Alkaline phosphatase (AP) is a universal stem cell membrane marker. The undifferentiated state of hESCs is characterized by high level of expression of AP. This video shows the steps of AP stain.

    Publish: 2012/5/2

  • Antibody Array for Protein Expression Profiling

    Abnova offers a variety of arrays for protein expression profiling. These arrays are designed for researchers to study highly relevant proteins in the specific research fields like Angiogenesis, Apoptosis, Cancer Marker, Cell Cycle, Cytokine, Hematopoiesis, Hormone, Signal Transduction and Stem Cell.

    Publish: 2009/11/10

  • Antibody Conjugation - FITC

    The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability.

    Publish: 2010/4/26

  • Antibody Purification (Affinity)

    Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix.

    Publish: 2010/4/26

  • Antibody Purification (Protein A Column)

    Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.

    Publish: 2010/2/8

  • Anti-PEG Antibody Pair

    Polyethylene Glycol (PEG) is a long chain polymer that has been approved by the Food and Drug Administration for human intravenous, oral and dermal applications. Covalent attachment of PEG (PEGylation) to proteins can reduce their immunogenicity, minimize proteolytic cleavage and increase their serum half-life. PEG has also been attached to small molecules and liposomes for more selective delivery. PEG-modification of superparamagnetic iron oxide and quantum dots can improve their biocompatibility and reduce non-specific uptake. This video shows the procedure of sandwich ELISA assay for anti-PEG antibody pair.

    Publish: 2011/6/29

  • Atomic Force Microscopy(AFM) Basic Tutorial

    Atomic force microscopy (AFM) is a very high-resolution type of scanning probe microscopy, with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit. The AFM is one of the foremost tools for imaging, measuring, and manipulating matter at the nanoscale. This video shows the AFM basic tutorial.

    Publish: 2014/3/13

  • BAD in Cancer Signalling

    BAD (Bcl-2 antagonist of cell death) belongs to the pro-apoptotic BH3-only subfamily of Bcl- 2 proteins. Physiological activity of BAD is highly controlled by phosphorylation. To further analyze the regulation of BAD function, the authors (Polzien L, Baljuls A, Albrecht M, Hekman M, Rapp UR) investigated the role of recently identified phosphorylation sites on BAD-mediated apoptosis.

    Publish: 2011/3/25

  • Calcium Phosphate Transfection

    Calcium phosphate transfection method is a simple, time efficient and relatively inexpensive way to introduce DNA into cells in stable cell lines such as HEK293T, CHO and BHK cells. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.

    Publish: 2010/7/5

  • Cell Thawing

    The thawing procedures for regular (non-DMSO-sensitive) cells are performed. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells.

    Publish: 2011/3/11

  • Cell Thawing for DMSO-Sensitive Cells

    This AbVideo describes how to thaw DMSO-sensitive cells. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells.

    Publish: 2011/3/11

  • Cri du Chat Syndrome

    Cri du chat syndrome is a a rare genetic disorder due to a missing part (deletion) of chromosome 5. Cri du chat syndrome was first described by a French geneticist, Jerome Lejeune in 1963 and named after the French term "cry or call of the cat" referring to the characteristic high-pitched cat-like cry of affected children. Here we present a brief introduction to the common knowledge of Cri du chat syndrome.

    Publish: 2014/11/11

  • Fluorescent Dye - Introduction

    As a novel product line, Abnova provides a complete selection of fluorescent labels covering the full working range of wavelengths, from 400nm to 850nm. The structures of our dyes are designed so as to provide great brightness and photo-stability, for high quality molecular imaging. Each fluorescent compound is available with many different reactive moieties, while retaining high photophysical features and different charge values (anionic, cationic, zwitterionic and neutral). Please visit the webpage "Fluorescent Dyes" for more information. Here we present a brief introduction to our fluorescence dye system.

    Publish: 2014/8/5

  • Fluorescent Dye - Protein Labeling

    After an introduction on Abnova’s fluorescent dye, this AbVideo shows you how easily it is to utilize Abnova’s kit to perform antibody labeling. As a novel product line, Abnova provides a complete selection of fluorescent labels covering the full working range of wavelengths, from 400nm to 850nm. The structures of our dyes are designed so as to provide great brightness and photo-stability, for high quality molecular imaging. As a more convenient and efficient way for fluorescent protein labeling, Abnova also provides our series of fluorescent dyes in kit format. Please visit the webpage "Fluorescent Dyes" for more information. Here is a brief introduction to why you should choose our products for your fluorescence labeling.

    Publish: 2014/8/12

  • GST Enzymatic Digestion

    GST fusion proteins allow convenient affinity purification of many proteins of interest but the GST-tag may become inconvenient in various downstream applications. We use PreScission protease to digest the GST fusion proteins and the protein without GST-tag is obtained.

    Publish: 2010/3/15

  • GST-tagged Protein Purification

    Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. We use GST-fusion protein to purify and detect proteins of interest. The GST-fusion protein can be purified from cells via its high affinity for glutathione.

    Publish: 2010/2/8

  • Immunoprecipitation

    Immunoprecipitation (IP) is the technique of precipitating an protein antigen out of solution using an antibody specific to that antigen. This antibody is immobilized on a solid-phase substrate such as protein A/G agarose beads. The beads are then added to the protein mixture and the proteins that are targeted by the antibodies are captured onto the beads.

    Publish: 2011/4/19

  • MaxBead Carboxyl Labeling Kit Operation

    MaxBead Carboxyl Labeling Kit provides whole set of materials for researchers to bind the desired ligand onto magnetic beads conveniently. If only the ligand contain primary amine groups such as antibody, protein, or peptide, it can be conjugated to the magnetic beads easily.

    Publish: 2014/5/13

  • MaxBead Protein A/G Operation

    MaxBead Protein A/G is designed as a rapid and simple tool for immunoprecipitation, purification/depletion assays, and other magnetic separation applications. Antibody can easily bind to the magnetic beads due to its high affinity with protein A/G.

    Publish: 2014/5/20

  • Microwestern Arrays - Lecture Series 1

    Lecture series 1 compares different array technologies and reveals their limitations and current challenges which brings out the importance of microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 2

    Lecture series 2 explicitly explains the step-by-step guide of performing microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 3

    Lecture series 3 demonstrates Dr. Chuu’s microwestern array experiments in details and also explains his results using different analyses. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 4

    Lecture series 4 points out the future direction and potential application using microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • mRNA Extraction

    Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

    Publish: 2010/4/19

  • Mycoplasma Assay

    Mycoplasma is highly infectious and cross-contamination commonly occurs when new cells are introduced into laboratories. We use PCR-based method for detection of Mycoplasma weekly. It is a highly sensitive, specific and rapid method to detect mycoplasma contamination in cell cultures.

    Publish: 2010/3/22

  • Optical Fiber Cleavage

    This AbVideo demonstrates the operation of automated fiber cleavage to produce flat and angled cleaves on fibers ranging from 80 microns to 1.25 mm in diameter for following biological applications. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/3

  • Optical Fiber End-Face Analysis

    This AbVideo demonstrates the operation of automated interferometric inspection system specifically designed for checking the surface quality and flatness of cleaved, polished or lensed fibers. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/10

  • PCR Purification

    Following PCR, you need to get rid of excess short primers, dNTPs, enzymes, short-failed PCR products and salts. We use a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O to get clean PCR products for downstream applications.

    Publish: 2010/3/22

  • Plasmid DNA Extraction (CsCl Isolation)

    CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method.

    Publish: 2011/8/30

  • Protemist® DT - Automated Wheat Germ Protein Synthesizer

    Automated protein synthesizer is an expert system in transcription, translation, and purification of recombinant protein. It is based on the eukaryotic translation apparatus of wheat germ. This high throughput system produces recombinant proteins in vitro. The generated proteins display foldings and biological functions similar to the mammalian counterparts.

    Publish: 2010/1/18

  • Protemist® DT II and XE - Demonstration

    The demonstration for Protemist® DT II - High Throughput Protein Expression and Protemist® XE - Large-Scale Protein Expression.

    Publish: 2010/9/24

  • Protemist® DT II and XE - Lecture Series

    Dr. Tomohisa Satoh presented two new generation, fully automated robotic desktop wheat germ cell-free protein synthesizers : Protemist® DT II (High Throughput) and Protemist® XE (Large-scale).

    Publish: 2010/9/24

  • Protemist® DT II - High Throughput Protein Expression

    This is an introduction on Protemist® DT II, a new generation, fully automated, robotic desktop protein synthesizer. It synthesizes protein of interest using wheat germ cell-free system and through bi-layer reaction. ProtemistR DT II can run two scales of transcription and translation reactions; either 1.2 ml or 6 ml. It is an expert system in transcription, translation, and purification.

    Publish: 2010/7/19

  • RNA Extraction from Cell Culture

    Extracting RNA from cultured cells has traditionally been a highly repetitive method, and this AbVideo indicates the steps on how to do it. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2010/4/19

  • Sandwich ELISA - Isotype Detection

    Sandwich ELISA is performed to determine the amount and serological class of antibodies made by an immunized animal or present in the serum of patients. The anti-immunoglobulin antibodies used have high specificity and sensitivity.

    Publish: 2010/6/30

  • Tissue Microarray

    Tissue Microarray is a powerful new technology for high throughput analysis of protein expression in a large number of tissue samples. Hundreds of tissue cores are arranged on a single slide, and then analyzed by immunohistochemistry staining. We offer a large collection of immmunohistochmistry (IHC) validated antibodies for the tissue microarray platform.

    Publish: 2009/12/8

  • UV-Vis Spectrophotometer - Nanodrop®

    The Thermo Scientific Nanodrop® is a full-spectrum spectrophotometer that measures 1 ul samples with high accuracy and reproducibility. It utilizes a sample retention technology that eliminates the need for cumbersome cuvettes and allows for clean up in seconds.

    Publish: 2010/10/11

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