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  • 2D Gel Electrophoresis (1) Protein Extraction

    Pure protein is the preliminary requirement of performing successful 2D gel electrophoresis. Our scientist showed you how to extract your proteins of interest by using a protein extraction kit followed by isopropanol precipitation and acetone wash steps. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/25

  • 2D Gel Electrophoresis (11) Mass Sample Preparation

    Mass Sample Preparation AbVideo focuses on the workflow of mass spectrometry before samples analysis. The entire process includes protein bands excision, organic solvent wash, in-gel digestion and peptide extraction. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/5/17

  • Adenoviral Vector Production

    This video demonstrates how to produce adenoviral vector after adenoviruses infection. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.

    Publish: 2011/6/17

  • Adenovirus Infection

    This video shows you how to infect culture cells with adenoviruses. It is an important step before the production of adenoviral vector. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.

    Publish: 2011/5/31

  • Agarose DNA Gel Preparation (by BCRC)

    This AbVideo is contributed by Bioresource Collection and Research Center and provides thorough instructions on preparing the agarose DNA gel. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.

    Publish: 2012/5/3

  • Angelman Syndrome

    Angelman Syndrome is characterized by severe developmental delay, speech impairment, gait ataxia and/or tremulousness of thelimbs, and a unique behavioral phenotype that includes happy demeanor and excessive laughter. In principle, its resulted from the deregulation of UBE3A gene located on chromosome 15. Here we present a brief introduction to the common knowledge of Angelman Syndrome.

    Publish: 2014/11/4

  • Antibody Purification (Protein A Column)

    Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.

    Publish: 2010/2/8

  • Blot Imaging with X-Ray Film

    An X-ray film can detect both the radioactive and chemiluminescent signals from Western, Northern or Southern blot. It is placed against the blot membrane in a light-proof cassette. After the exposure for an appropriate time period, the film is developed by an auto-processor in a darkroom.

    Publish: 2011/2/16

  • Calcium Phosphate Transfection

    Calcium phosphate transfection method is a simple, time efficient and relatively inexpensive way to introduce DNA into cells in stable cell lines such as HEK293T, CHO and BHK cells. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.

    Publish: 2010/7/5

  • Cationic Polymer Transfection

    jetPEI™, a polyethylenimine (PEI) derivative, is a cationic polymer transfection reagent that binds to DNA by electrostatic forces. The cationic polymer/DNA complex enters the cell by endocytosis, where the DNA is then released into the cytoplasm.

    Publish: 2011/2/11

  • Cell Culture (Suspension Cell)

    Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture suspension cells from cell thawing, cell maintenance, to cell freezing.

    Publish: 2010/3/1

  • Cell Culture Medium Preparation

    This video demonstrates the steps to prepare cell culture medium from media powder.

    Publish: 2011/4/19

  • Cell Freezing Equipment - Mechanical Freezer

    How to transport cryovials from 4°C, -20°C, -80°C mechanical freezers to liquid nitrogen tank while maintaining optimal cell health is presented in this video. Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. The cryopreservation agent and condition can depend on cell type.

    Publish: 2011/5/27

  • Cell Nuclear Protein Extraction

    Nuclear protein fractionation is proposed as a method to be carried out according to the solubility of proteins in buffers of increasing ionic strength. This protocol indicates the procedure for the preparation of nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer.

    Publish: 2010/5/31

  • Chicken Antibody Extraction

    This episode on chicken antibody describes the antibody extraction process. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to Chicken Antibody Extraction.

    Publish: 2014/7/29

  • Chimera RNA Interference

    Chimera RNAi is a process by which small interfering RNA/DNA chimera triggers the destruction of mRNA. The discovery work, design, and application of chimera RNAi have been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.

    Publish: 2010/8/9

  • Competent Cell Preparation

    Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. Because DNA is hydrophilic, it will not normally pass through membrane. In order to make these cells readily incorporate foreign DNA, they must first be made "competent" to take up foreign DNA.

    Publish: 2010/7/5

  • Covalently Closed Circular DNA (cccDNA) Isolation

    cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.

    Publish: 2011/6/29

  • Cre-Lox Recombination

    Cre-Lox recombination is a special type of site-specific recombination. The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. It is often used in the generation of knockout and conditional knockout animals.

    Publish: 2010/4/12

  • CytoQuest™ CR CTC Automated Leucosep Preparation

    An automated system performing PBMC extraction is shown in this AbVideo. After Leucosep preparation, cells can either be banked or followed by downstream CTC enumeration using CytoQuest™ CR. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/1/13

  • CytoQuest™ CR CTC Leucosep Preparation

    Prior to CTC enumeration by CytoQuest™ CR, blood sample pre-treatment involving cell separation and PBMC extraction from whole blood are necessary. This AbVideo shows detailed Leucosep preparation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/31

  • DEPC Treatment

    Diethylpyrocarbonate (DEPC) is used in the laboratory to inactivate the RNase enzymes from water and other laboratory utensils. It inactivates the RNases by the covalent modifications of the histidine residues. DEPC treated (and therefore RNase-free) water is used in handling of RNA to reduce the risk of RNA being degraded by RNases.

    Publish: 2011/4/19

  • Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)

    Human umbilical vein endothelial cells (HUVEC) are commonly used as a laboratory model system for the physiological and pharmacological investigations. This video describes how to derive HUVEC from the endothelium of veins of the umbilical cord.

    Publish: 2013/3/29

  • DNA Electrophoresis

    DNA electrophoresis is an analytical technique used to separate DNA fragments based on size, electrical charge and other physical properties. The DNA separates out into bands, with the distance from the electrode corresponding to length of the strand. The technique plays a role in identifying genes for diagnosing disease and for other forms of genetic research.

    Publish: 2010/10/25

  • DNA Extraction by Phenol/Chloroform

    This method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing phenol and chloroform, resulting in an upper aqueous phase and a lower organic phase (mainly chloroform). DNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

  • DNA Extraction from Mouse Tail (Manual)

    DNA extraction is a routine procedure to collect DNA for subsequent molecular analysis. This video shows you how to extract DNA from mouse tails. There are three basic steps: (i) break cells by lysis buffer, (ii) digest protein and remove contamination by proteinase, (iii) precipitating the DNA.

    Publish: 2010/8/30

  • DNA Gel Preparation

    This AbVideo demonstrates step by step of the agarose gel preparation for DNA electrophoresis. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.

    Publish: 2010/10/4

  • DNA Sequencing

    DNA sequencing is a technique to determine the order of the nucleotide bases-adenine, guanine, cytosine, and thymine. It is indispensable for basic biological research and discovery.

    Publish: 2010/1/14

  • Edwards Syndrome

    Edwards syndrome, also known as trisomy 18, is a genetic disorder resulted from the somatic aneuploidy with an extra chromosome 18. It is typically associated with significant infant mortality. Edwards syndrome is the second most common somatic aneuploidy after Down syndrome. Here we present a brief introduction to the common knowledge of Edwards syndrome.

    Publish: 2014/9/23

  • Electroporation

    Electroporation applies a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules to pass into the cell. It is usually used in molecular biology as a way of introducing some substance into a cell, such as molecular probes, drugs or pieces of coding DNA.

    Publish: 2010/11/22

  • Fluorescence In Situ Hybridization (FISH)

    FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. You can use FISH probes for the detection of gene amplification, loss and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library.

    Publish: 2011/5/5

  • Fluorescent Dye - Differential Gel Electrophoresis

    This episode of fluorescent dye discusses the theory behind differential gel electrophoresis (DGE) and the advantages of using Abnova’s DGE labeling kit. As an alternative application, 2D-PAGE electrophoresis represents a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. Abnova presents a series of DGE labeling kits for faster, easier and more accurate quantification in 2D-PAGE. Please visit the webpage "DGE Labeling Kit" for more information. Here is a brief introduction to why you should choose our products for your fluorescence labeling.

    Publish: 2014/8/19

  • Fluorescent Dye - Introduction

    As a novel product line, Abnova provides a complete selection of fluorescent labels covering the full working range of wavelengths, from 400nm to 850nm. The structures of our dyes are designed so as to provide great brightness and photo-stability, for high quality molecular imaging. Each fluorescent compound is available with many different reactive moieties, while retaining high photophysical features and different charge values (anionic, cationic, zwitterionic and neutral). Please visit the webpage "Fluorescent Dyes" for more information. Here we present a brief introduction to our fluorescence dye system.

    Publish: 2014/8/5

  • Fluorescent Dye - Protein Labeling

    After an introduction on Abnova’s fluorescent dye, this AbVideo shows you how easily it is to utilize Abnova’s kit to perform antibody labeling. As a novel product line, Abnova provides a complete selection of fluorescent labels covering the full working range of wavelengths, from 400nm to 850nm. The structures of our dyes are designed so as to provide great brightness and photo-stability, for high quality molecular imaging. As a more convenient and efficient way for fluorescent protein labeling, Abnova also provides our series of fluorescent dyes in kit format. Please visit the webpage "Fluorescent Dyes" for more information. Here is a brief introduction to why you should choose our products for your fluorescence labeling.

    Publish: 2014/8/12

  • Gateway® Recombination

    The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. In contrast, Gateway recombination cloning uses a one hour, 99%-efficient, reversible recombination reaction, without using restriction enzymes, ligase, and subcloning step. It is an extremely fast and efficient way to clone your gene.

    Publish: 2010/4/12

  • Gel Extraction

    Gel extraction is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. Three basic steps are involved: slicing the bands of interest on UV light exposure, isolating the DNA from those bands, and removing the accompanying salts and stain.

    Publish: 2010/5/24

  • Gene Intron-Exon

    The Reference Sequence (RefSeq) database is a non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa. RefSeq also reports the information about exon and intron boundaries and length.

    Publish: 2010/10/21

  • Gene ORF Finder

    ORF Finder identifies all possible ORFs in a DNA sequence by locating the standard and alternative stop and start codons. The deduced amino acid sequences can then be used to BLAST against GenBank.

    Publish: 2010/10/14

  • GST-tagged Protein Purification

    Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. We use GST-fusion protein to purify and detect proteins of interest. The GST-fusion protein can be purified from cells via its high affinity for glutathione.

    Publish: 2010/2/8

  • Insect Cell Culture

    The Sf9 cell line derived from pupal ovarian tissue of the Fall armyworm Spodoptera frugiperda is one of the most common cell lines used for BEVS (Baculovirus expression vector systems). This video shows you how to culture the Sf9 cell line.

    Publish: 2010/5/31

  • Invasion Assay

    Cell invasion assay is designed to accelerate the screening process for compounds that influence cell migration through extracellular matrices. Matrigel (BD), extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, resembles the extracellular matrices is used by cell biologists as a substrate in cell invasion assay.

    Publish: 2010/11/12

  • Isotope Labeling

    Isotopic labeling is a technique for labeling a substance with a stable or radioactive isotope. By measuring the radioactivity or the abundance of the isotope, we could made observations of the course through a biologic process. This video shows the procedure for using radioisotope phosphorus-32 to label DNA.

    Publish: 2011/7/1

  • Methylation-specific PCR

    Methylation-specific PCR (MSP) is a rapid and inexpensive method that can be used to determine the methylation status of DNA. It is a bisulfite conversion based PCR technique. In MSP, two primer pairs are designed for methylated and unmethylated DNA.

    Publish: 2010/8/5

  • Micro-Manipulator - Probe Preparation

    Precise physical interactions under microscopic level turn out to be reachable because of the aid from micromanipulator. In concert with hydraulic drive systems, it is principally the glass probe that makes the physical contact with target cell for purposes like single cell collection, injection, patch clamping, etc. Here we present you step by step of probe preparation including how to do glass capillary pulling and micropipette tip processing. Instruments adopted here are MF-900 microforge and PC-10 puller, both from NARISHIGE Group.

    Publish: 2014/4/15

  • Micro-Manipulator - Single Cell Collection

    With the aid of micromanipulator, precise physical interactions, like single cell collection, injection, patch clamping, etc. under microscopic level becomes achievable. In general, depending on the application, one or more micromanipulators can be docked on a microscope stage to operate in microscopes field of view. A typical method for single cell collection is displayed including steps of cell aspiration and discharge. And, MMO-203 Three-axis Oil Hydraulic Micromanipulator from NARISHIGE Group is adopted here.

    Publish: 2014/4/22

  • Micropipette Calibration

    Micropipettes are used to accurately measure and dispense small volumes of liquid. The capacity of a micropipette can range from less than 1μl to 1000μl (1ml). To ensure that obtained results using micropipette are reliable, it is necessary to calibrate micropipettes several times a year. This video shows a method for testing the accuracy of automatic micropipettes.

    Publish: 2010/7/19

  • Miller-Dieker Syndrome

    Miller-Dieker syndrome is an autosomal dominant congenital disorder characterized by lissencephaly (smooth brain), which is resulted from incomplete neuronal migration. The disorder arises from the deletion of part of the small arm of chromosome 17p (which includes both the LIS1 and 14-3-3 epsilon gene), leading to partial monosomy. Here we present a brief introduction to the common knowledge of Miller-Dieker syndrome.

    Publish: 2014/12/2

  • Mononuclear Cell Isolation from Whole Blood

    This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps.

    Publish: 2010/5/31

  • Mouse Genotyping

    Genotyping is the process of determining the genes (genotype) of an individual by examining its DNA with the use of biological assays (ex: PCR, DNA sequencing). Three steps to type the mouse genotype : (i) isolate the genomic DNA from mouse tails; (ii) PCR to amplify with specific primers; (iii) electrophoresis to determine the genotype.

    Publish: 2010/8/26

  • mRNA Extraction

    Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

    Publish: 2010/4/19

  • Mycoplasma Detection - Hoechst DNA Staining

    This AbVideo tells you how to detect Mycoplasma in vero cell samples through the “Hoechst DNA staining method”, since one of the most important cell culture procedures is to use microtest to identify whether mycoplasmal contaminant is present or not.

    Publish: 2011/8/8

  • Nuclear and Cytoplasmic Protein Extraction

    Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells.

    Publish: 2011/4/19

  • Oil Red O Staining for Adipogenesis

    Oil Red O is a lysochrome diazo dye used for demonstrating neutral triglycerides and lipids in tissue. This video shows the procedure of Adipogenesis staining with Oil Red O.

    Publish: 2013/4/8

  • Optical Fiber Cleavage

    This AbVideo demonstrates the operation of automated fiber cleavage to produce flat and angled cleaves on fibers ranging from 80 microns to 1.25 mm in diameter for following biological applications. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/3

  • Particle Blot for HBV Particle

    Hepatitis B viruses (HBV) are double-stranded DNA viruses that cause acute and chronic hepatitis. Furthermore, HBV infection is the leading cause of liver cancer. This video shows the procedure for using particle blot to analyze HBV Particle.

    Publish: 2011/6/17

  • Patau Syndrome

    Patau Syndrome, resulted from chromosome 13 trisomy, is regarded the least common but the most severe among those somatic aneuploidies. It is typically associated with significant infant mortality. Clinical features of Patau syndrome include delayed development with central nervous system anomalies, midline facial defects, and urogenital malformations. Medical management of children with Trisomy 13 should be planned on a case-by-case basis according to the individual circumstances of the patient. Here we present a brief introduction to the common knowledge of Patau Syndrome.

    Publish: 2014/10/1

  • PCR Cloning

    PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid.

    Publish: 2010/3/31

  • PCR Cloning (I) - Inserted Fragment Preparation

    The procedure of PCR cloning includes DNA preparation, digestion, ligation and transformation. This video shows the first part- insert PCR. Compared to other Taq-generated PCR inserts, Pfu DNA polymerase-generated PCR fragments will have fewer errors and is more commonly used for molecular cloning.

    Publish: 2010/3/22

  • PCR Cloning (II) - Digestion

    Digestion is the process of cutting DNA molecules with restriction endonucleases. These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA. In PCR cloning, insert and vector are cutted by the same restriction endonucleases and then join together in the next step, ligation.

    Publish: 2010/3/29

  • PCR Cloning (III) - Ligation

    DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3 hydroxyl ends of one nucleotide with the 5 phosphate end of another. In PCR cloning, insert DNA and vector digested by the same restriction enzyme can be ligated by DNA ligase.

    Publish: 2010/3/29

  • PCR Cloning (IV) - Transformation

    The purpose of transformation is to introduce a foreign plasmid into bacteria and the bacteria will replicate the foreign plasmid along with their own DNA. Bacteria which are able to uptake DNA after a heat shock are called "competent".

    Publish: 2010/3/29

  • PCR Cloning (V) - Colony Selection

    It can be used after a transformation to screen colonies for the plasmid. Primers designed for the insert sequence should be used when preparing the PCR reaction. Thus, any colonies which give rise to an amplification product are likely to contain the correct DNA sequence.

    Publish: 2010/3/29

  • PCR Mutagenesis

    PCR can be used to make changes to the nucleotide sequence of DNA. This is called PCR mutagenesis. Site-direct mutagenesis is one kind of PCR mutagenesis, which introduces a mutation at a specific location on the DNA strand.

    Publish: 2010/7/5

  • PCR Purification

    Following PCR, you need to get rid of excess short primers, dNTPs, enzymes, short-failed PCR products and salts. We use a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O to get clean PCR products for downstream applications.

    Publish: 2010/3/22

  • Plasmid DNA Extraction (CsCl Isolation)

    CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method.

    Publish: 2011/8/30

  • Plasmid DNA Extraction (Gigaprep)

    Gigaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 2.5~5 L of LB broth and the expected DNA yield is 7.5~10 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes megaprep, midiprep, and miniprep.

    Publish: 2010/5/24

  • Plasmid DNA Extraction (Megaprep)

    Megaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 500 ml~2.5 L of LB broth and the expected DNA yield is 1.5~2.5 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, midiprep, and miniprep.

    Publish: 2010/5/24

  • Plasmid DNA Extraction (Midiprep)

    Midiprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 15-25 ml of LB broth and the expected DNA yield is 100-350 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and miniprep.

    Publish: 2010/5/17

  • Plasmid DNA Extraction (Miniprep)

    Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and midiprep

    Publish: 2010/4/26

  • Plasmid DNA Extraction (Traditional Alkaline Lysis Method)

    Alkaline lysis is one of the most generally useful methods for isolating circular plasmid DNA from bacterial cells. This video demonstrates the procedures for alkaline lysis method.

    Publish: 2011/8/2

  • Polymerase Chain Reaction

    Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles.

    Publish: 2010/1/7

  • Propidium Iodide Staining

    Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule that can be used to stain DNA. PI is used as a DNA stain for both flow cytometry to evaluate cell viability or DNA content in cell cycle analysis and microscopy to visualize the nucleus and other DNA containing organelles. It can be used to differentiate necrotic, apoptotic and normal cells.

    Publish: 2010/12/10

  • Protein Quantification (BCA Assay)

    The bicinchoninic acid assay (BCA Assay) is a biochemical assay for determining the total level of protein in a solution, similar to Bradford protein assay. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

    Publish: 2010/5/24

  • Protein Quantification (Bradford Assay)

    Bradford assay is a rapid and accurate method to determine the concentration of protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mg/ml), the more protein present, the more Coomassie binds.

    Publish: 2010/3/15

  • Protein Structure

    "Entrez Structure", also known as Molecular Modeling DataBase (MMDB), is a database of experimentally determined structures obtained from the RCSB Protein Data Bank (PDB). It provides a wealth of information on the biological function, on mechanisms linked to the function, and on the evolutionary history of relationships between macromolecules.

    Publish: 2010/9/30

  • PubMed Literature Database

    PubMed is a free literature database which comprises more than 20 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

    Publish: 2010/10/25

  • Real-Time PCR

    Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample.

    Publish: 2010/4/19

  • Reverse Transcription PCR

    Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR.

    Publish: 2010/4/19

  • RNA Extraction by TRIzol®

    TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

  • RNA Extraction from Cell Culture

    Extracting RNA from cultured cells has traditionally been a highly repetitive method, and this AbVideo indicates the steps on how to do it. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2010/4/19

  • RNA Extraction from Tissue

    Difficulties of isolating intact total RNA from tissue samples vary with the physical and biochemical nature of tissue. This AbVideo demonstrates how we can extract total RNA from tissue. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2010/4/19

  • RNA Gel Preparation

    The quality of RNA preparation could be measured by electrophoresis on a denaturing agarose gel. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the formaldehyde agarose gel for the electrophoresis of RNA.

    Publish: 2011/1/6

  • Southern Blot

    The Southern blot is used to detect a specific DNA sequence in DNA samples. A general blotting procedure starts with the separation of digested DNA fragments. The DNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.

    Publish: 2011/2/16

  • Syringe Filter

    A syringe filter is a single-use filter cartridge. It can be used to remove particles from a sample. We’ll show you how to use a syringe filter.

    Publish: 2011/4/19

  • Trisomy X Syndrome

    Trisomy X Syndrome, resulted from sex chromosome X trisomy, is regarded the most common female aneuploidies. It is characterized with a broad spectrum of phenotypic variations. Although with different severity, most patients have normal lifespan as general public. Here we present a brief introduction to the common knowledge of Trisomy X Syndrome.

    Publish: 2014/10/9

  • Warsaw Breakage Syndrome

    Cohesinopathies are human developmental disorders caused by inherited defects in cellular components controlling the process of sister chromatid cohesion. This cohesion mechanism takes care of keeping the sister chromatids close together from the stage of DNA replication up until mitosis. Two syndromes were described so far in the group of cohesinopathes: Roberts syndrome and Cornelia de Lange syndrome. In Van der Lelij research, a third cohesinopathy is added to this group: The Warsaw Breakage syndrome, caused by mutations in DDX11/ChlR1.

    Publish: 2010/8/31

  • Western Blot - Ponceau S Staining

    Ponceau S is a rapid and reversible staining method for locating protein bands on Western blots producing reddish pink stained bands. The stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and can be completely removed from the protein bands by continued washing.

    Publish: 2010/11/26

  • Western Blot Membrane Stripping

    Stripping is used to remove primary and secondary antibodies from a western blot membrane to allow the incubation of new antibodies. It is useful to save samples, materials, and time when you want to investigate more than one protein on the same blot.

    Publish: 2010/12/17

  • Wolf-Hirschhorn Syndrome

    Resulted from the micro-deletion of the Wolf-Hirschhorn syndrome critical region (WHSC1 and WHSC2) located on the short arm of chromosome 4 (4p16.3), Wolf-Hirschhorn syndrome is a rare congenital disorder with estimated frequency of 1:50000 births. Here we present a brief introduction to the common knowledge of Wolf-Hirschhorn syndromee.

    Publish: 2014/12/30

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