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  • Covalently Closed Circular DNA (cccDNA) Isolation

    cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.

    Publish: 2011/6/29

  • CytoQuest™ CR CTC Automated Leucosep Preparation

    An automated system performing PBMC extraction is shown in this AbVideo. After Leucosep preparation, cells can either be banked or followed by downstream CTC enumeration using CytoQuest™ CR. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/1/13

  • CytoQuest™ CR CTC Capture

    CytoQuest™ CR CTC Capture AbVideo focuses on system operation including processes on parameters and settings, system priming, buffer filling, sample loading and capturing as well as cell fixation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/2/3

  • CytoQuest™ CR CTC Capture and CTC FISH - Dos and Don`ts

    The AbVideo on CytoQuest™ CR Dos and Don’ts part 3 points out few precautions one should pay attentions to when performing capture antibody immobilization, CTC capture and CTC FISH slide preparation.

    Publish: 2015/3/24

  • CytoQuest™ CR CTC Capture Antibody Immobilization

    This AbVideo demonstrates step by step protocol of antibody immobilization on CytoChipNano before CTC capture. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/30

  • CytoQuest™ CR CTC FISH

    For the convenience of researchers, CytoChipNano can be used in fluorescence in situ hybridization (FISH) after immunostaining. Steps on detaching micromixer and applying FISH probes are presented in this AbVideo. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/1/20

  • CytoQuest™ CR CTC Immunostaining

    A thorough description on how to perform immunostaining directly on CytoChipNano after CTC capture is performed by CytoQuest™ CR. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/1/26

  • CytoQuest™ CR CTC Leucosep Preparation

    Prior to CTC enumeration by CytoQuest™ CR, blood sample pre-treatment involving cell separation and PBMC extraction from whole blood are necessary. This AbVideo shows detailed Leucosep preparation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/31

  • CytoQuest™ CR CTC Post-Leucosep Freezing and Banking

    This episode of CytoQuest™ CR CTC AbVideo provides procedures on cell freezing and banking after Leucosep preparation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/30

  • CytoQuest™ CR System Maintenance

    This AbVideo provides instructions on maintaining CytoQuest™ CR to avoid contaminations and ensure prolong usage. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/30

  • Mononuclear Cell Isolation from Whole Blood

    This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps.

    Publish: 2010/5/31

  • Mycoplasma Detection Broth Preparation

    Mycoplasma detection broth (without crystal violet) is used for the isolation and cultivation of Mycoplasma for research studies. This video shows you how to prepare the Mycoplasma broth.

    Publish: 2011/12/2

  • Plasmid DNA Extraction (CsCl Isolation)

    CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method.

    Publish: 2011/8/30

  • RNA Extraction by TRIzol®

    TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

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