AbVideo™ Browser

  • Where to buy
  • Choose your location

AbVideo™

   
  • 2D Gel Electrophoresis (1) Protein Extraction

    Pure protein is the preliminary requirement of performing successful 2D gel electrophoresis. Our scientist showed you how to extract your proteins of interest by using a protein extraction kit followed by isopropanol precipitation and acetone wash steps. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/25

  • 2D Gel Electrophoresis (10) Visible Staining

    In addition to the fluorescent stain, the result of the 2D gel electrophoresis can also be visualized with visible stain. This video demonstrates the step-by-step procedure using VisPROTM to detect 2D gel result on the Gel Lighting Plate. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/4/8

  • 2D Gel Electrophoresis (11) Mass Sample Preparation

    Mass Sample Preparation AbVideo focuses on the workflow of mass spectrometry before samples analysis. The entire process includes protein bands excision, organic solvent wash, in-gel digestion and peptide extraction. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/5/17

  • 2D Gel Electrophoresis (12) Mass Analysis

    This video shows how to use MALDI-TOF system to present mass analysis. The mass spectrometry data can be blasted with online database to identify proteins. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/5/17

  • 2D Gel Electrophoresis (2) Protein Quantitation

    Extracted proteins can be resuspended in rehydration buffer and quantified by using Bradford assay. This AbVideo introduces the detailed procedure of measuring the protein concentration in a solution. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/25

  • 2D Gel Electrophoresis (3) Sample Preparation for the First Dimension

    Learn how IEF Optimizer can be used for measuring sale content of the protein samples and calculating the suggested total voltage hour for IEF process with online program. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/25

  • 2D Gel Electrophoresis (4) The First Dimension

    Step-by-step protocol of setting the 1st dimension experiment of 2D Gel Electrophoresis is introduced in this video. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/25

  • 2D Gel Electrophoresis (5) Gel Casting

    This Abvideo demonstrates the preparation and storage procedure of gel casting. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/25

  • 2D Gel Electrophoresis (6) Reduction and Alkylation

    The reduction and alkylation step is required prior to any electrophoretic step to reduce the number of false spots. Here we show you the standard process to perform this step. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/3/31

  • 2D Gel Electrophoresis (7) The Second Dimension

    A thorough demonstration on how to perform the 2nd dimension of 2D gel electrophoresis is provided in this video. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/4/8

  • 2D Gel Electrophoresis (8) Fluorescent Staining

    SyproRuby stain is a sensitive and convenient fluorescent stain for the detection of proteins separated by gel electrophoresis. This video demonstrates the standard procedure of fluorescent staining that includes overnight staining process and the following wash steps. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/4/8

  • 2D Gel Electrophoresis (9) Image Capture - Laser Scanner

    Following the gel staining step, image results of a 2D gel can be captured by using a TyphoonTM Laser Scanner to capture is shown in this AbVideo. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/4/8

  • 2D Gel Electrophoresis (9) Image Capture - UV Light Box

    Besides using the laser scanner to capture the 2D gel electrophoresis image, researchers can also use a UV light box to visualize separated proteins on their 2D gels. Learn how to capture the image in this tutorial. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).

    Publish: 2011/4/8

  • Adenoviral Vector Production

    This video demonstrates how to produce adenoviral vector after adenoviruses infection. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.

    Publish: 2011/6/17

  • Adenovirus Infection

    This video shows you how to infect culture cells with adenoviruses. It is an important step before the production of adenoviral vector. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.

    Publish: 2011/5/31

  • Aliquoting and Preparation of Serum before Usage

    This is recommended to creat aliquots of serum when only small volumes are used at a time. This video shows you how to thaw the serum, aliquot into the 50ml tubes and refreeze.

    Publish: 2011/5/31

  • Alizarin Red S Staining for Osteogenesis

    Alizarin Red is used to identify calcium deposits in tissue sections. Calcium forms an Alizarin Red S-calcium complex in a chelation process. This video describes the procedure of Alizarin Red S Staining for osteogenesis.

    Publish: 2013/4/8

  • Angelman Syndrome

    Angelman Syndrome is characterized by severe developmental delay, speech impairment, gait ataxia and/or tremulousness of thelimbs, and a unique behavioral phenotype that includes happy demeanor and excessive laughter. In principle, its resulted from the deregulation of UBE3A gene located on chromosome 15. Here we present a brief introduction to the common knowledge of Angelman Syndrome.

    Publish: 2014/11/4

  • Antibody Array for Protein Expression Profiling

    Abnova offers a variety of arrays for protein expression profiling. These arrays are designed for researchers to study highly relevant proteins in the specific research fields like Angiogenesis, Apoptosis, Cancer Marker, Cell Cycle, Cytokine, Hematopoiesis, Hormone, Signal Transduction and Stem Cell.

    Publish: 2009/11/10

  • Antibody Conjugation - Biotinylation

    Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. A biotin/streptavidin (avidin) system offers low background, enhanced sensitivity and the ability to detect minute amounts of antigens.

    Publish: 2010/4/26

  • Antibody Conjugation - FITC

    The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability.

    Publish: 2010/4/26

  • Antibody Pair for Proximity Ligation Assay

    The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation) in tissue and cell samples prepared for microscopy. Each detected signal is visualized as an individual fluorescent dot, these signals can be quantified (counted) and assigned to a specific subcellular location based on microscopy images.

    Publish: 2009/11/10

  • Antibody Purification (Affinity)

    Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix.

    Publish: 2010/4/26

  • Antibody Purification (Protein A Column)

    Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.

    Publish: 2010/2/8

  • Anti-PEG Antibody Pair

    Polyethylene Glycol (PEG) is a long chain polymer that has been approved by the Food and Drug Administration for human intravenous, oral and dermal applications. Covalent attachment of PEG (PEGylation) to proteins can reduce their immunogenicity, minimize proteolytic cleavage and increase their serum half-life. PEG has also been attached to small molecules and liposomes for more selective delivery. PEG-modification of superparamagnetic iron oxide and quantum dots can improve their biocompatibility and reduce non-specific uptake. This video shows the procedure of sandwich ELISA assay for anti-PEG antibody pair.

    Publish: 2011/6/29

  • Atomic Force Microscopy(AFM) Basic Tutorial

    Atomic force microscopy (AFM) is a very high-resolution type of scanning probe microscopy, with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit. The AFM is one of the foremost tools for imaging, measuring, and manipulating matter at the nanoscale. This video shows the AFM basic tutorial.

    Publish: 2014/3/13

  • Autoclave Quality Control Test - Biological Indicator

    Besides the temperature logger, self-contained biological indicators that contain G. stearothermophilus spores can also be used for autoclave quality control test. Learn how to use the biological indicator in this AbVideo. There are also physical and chemical indicators that can be used to ensure decontamination effectiveness of autoclaves.

    Publish: 2012/1/12

  • Autoclave Quality Control Test - Temperature Logger

    Stainless steel loggers are commonly used for monitoring temperature of autoclave. Check out how to use it to maintain the maximum performance of your autoclave machine. There are physical, chemical, and biological indicators that can be used to ensure decontamination effectiveness of autoclaves.

    Publish: 2012/1/12

  • Bacterial/Fungal Contaminant Detection

    It s very important to test cell lines and cell culture media to determine if microbial contamination is present. The culture media used for the tests are tryptic soy broth (TSB) for the detection of aerobic and anaerobic bacteria, and fluid thioglycollate medium (FTM) for the detection of erobes, facultative anaerobes and fungi.

    Publish: 2011/8/8

  • BD Matrigel™ Aliquoting

    This AbVideo shows you how to create aliquots of BD Matrigel when only small volumes are used at a time. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media.

    Publish: 2011/12/2

  • BD Matrigel™ Coating

    BD Matrigel Coating plays a basic but crucial role for BD Matrigel matrix, and this video presents you the steps of its procedure. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media.

    Publish: 2011/12/2

  • Bioburden Test of BSC

    Biological Safety Cabinets (BSC) is an enclosed, ventilated laboratory workspace which provides the most effective primary containment for working with infectious agents. The BSC is tested for bioburden to ensure the adequate protection.

    Publish: 2011/8/2

  • BioSpectrum® Imaging System

    The BioSpectrum® Imaging System is designed to automate the research with one-touch, pre-set or user-defined controls for accurate, repeatable imaging and analysis for chemiluminescent, bioluminescent, fluorescent, colorimetric imaging. The BioSpectrum® is faster than film for western blot imaging.

    Publish: 2010/9/13

  • BLAST - Multiple Alignment

    Multiple sequence alignment is an extension of pairwise alignment to incorporate more than two sequences at a time. It is often used to identify conserved sequence regions which are assumed to be evolutionarily related. By construct phylogenetic trees, it aids in establishing evolutionary relationships.

    Publish: 2010/7/26

  • BLAST - Nucleotide

    The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

    Publish: 2010/6/14

  • BLAST - PCR Primer Design

    Primer-BLAST was developed to help users make primers that are specific to the input PCR template. It uses Primer3 to design PCR primers and then submits them to BLAST search against user-selected database. The blast results are then automatically analyzed to avoid primer pairs that can cause amplification of targets other than the input template.

    Publish: 2010/7/29

  • BLAST - Protein

    BLAST-Protein (blastp) is used for both identifying a query amino acid sequence and for finding similar sequences in protein databases. Like other BLAST programs, blastp is designed to find local regions of similarity. When sequence similarity spans the whole sequence, blastp will also report a global alignment, which is the preferred result for protein identification purposes.

    Publish: 2010/7/29

  • Blood Smear

    A blood smear gives information about the number and shape of blood cells. To perform a blood smear, two very clean slides are required. Drop a small drop of blood on one of the slides. Pull blood forward across slide.

    Publish: 2010/8/30

  • Blot Imaging with X-Ray Film

    An X-ray film can detect both the radioactive and chemiluminescent signals from Western, Northern or Southern blot. It is placed against the blot membrane in a light-proof cassette. After the exposure for an appropriate time period, the film is developed by an auto-processor in a darkroom.

    Publish: 2011/2/16

  • Calcium Phosphate Transfection

    Calcium phosphate transfection method is a simple, time efficient and relatively inexpensive way to introduce DNA into cells in stable cell lines such as HEK293T, CHO and BHK cells. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.

    Publish: 2010/7/5

  • Cationic Polymer Transfection

    jetPEI™, a polyethylenimine (PEI) derivative, is a cationic polymer transfection reagent that binds to DNA by electrostatic forces. The cationic polymer/DNA complex enters the cell by endocytosis, where the DNA is then released into the cytoplasm.

    Publish: 2011/2/11

  • Cell Counting

    Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.

    Publish: 2011/3/11

  • Cell Counting - Cellometer®

    The Nexcelom Bioscience Cellometer® is a simple to use, automated cell counter designed to improve accuracy and repeatability. You just pipette 20μL of sample, insert the slide and click count. Cell Concentration, cell size and viability are displayed in less than 30 seconds.

    Publish: 2010/10/11

  • Cell Counting (Attached Cells)

    This episode of the cell counting series demonstrates the handling of attached cell before counting. Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.

    Publish: 2010/3/1

  • Cell Counting (Cell Suspension)

    This AbVideo introduces the protocol of preparing suspension cell for counting. Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.

    Publish: 2010/3/1

  • Cell Fixation

    The cells must be fixed and permeabilized to ensure free access of the antibody to its antigen. Fixation methods fall generally into two classes: organic solvents and cross-linking reagents. This video uses formaldehyde as a cross-linking reagent to fix cells.

    Publish: 2010/8/30

  • Cell Freezing Equipment - CoolCell®

    The "CoolCell" is an alcohol-free freezing container which provides the ideal freezing rate close to minus 1°C/minute required for successful cryopreservation of cells. This video demonstrates you the steps of how to use it.

    Publish: 2011/5/31

  • Cell Freezing Equipment - Mechanical Freezer

    How to transport cryovials from 4°C, -20°C, -80°C mechanical freezers to liquid nitrogen tank while maintaining optimal cell health is presented in this video. Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. The cryopreservation agent and condition can depend on cell type.

    Publish: 2011/5/27

  • Cell Freezing Equipment - Mr. Frosty™

    This AbVideo shows the procedure for using "Mr. Frosty". "Mr. Frosty" is a freezing container filled with isopropyl alcohol at room temperature. It offers the ideal cooling rate close to minus 1°C/minute required for effective cryopreservation of cells.

    Publish: 2011/5/27

  • Cell Freezing Equipment - Programmable Freezer

    This video displays the procedure for cell freezing with programmable freezer. By using programmable freezer, you could control and ensure the freezing rates are exactly as required. It is the most reliable and reproducible way to freeze cells.

    Publish: 2011/5/27

  • Cell Freezing Equipment - Styrofoam Box

    The Styrofoam box slows the rate of freezing, so that ice crystals won’t form too fast and destroy the cells. This AbVideo provides instructions on how to use Styrofoam box for cell freezing and shows the procedure for you.

    Publish: 2011/5/31

  • Cell Lysate Preparation (Denatured)

    Denaturation is a process in which proteins or nucleic acids lose their native structure due to external stress or compound. This AbVideo shows the protocol involves three steps for denatured lysate preparation. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, denatured lysate in 1x Sample Buffer.

    Publish: 2010/6/7

  • Cell Lysate Preparation (Native)

    Here we display you the protocol on how to prepare cell lysate. This includes 3 steps: First, cells are harvested. Then, cells are lysed in lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.

    Publish: 2010/6/7

  • Cell Nuclear Protein Extraction

    Nuclear protein fractionation is proposed as a method to be carried out according to the solubility of proteins in buffers of increasing ionic strength. This protocol indicates the procedure for the preparation of nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer.

    Publish: 2010/5/31

  • Cell Proliferation Assay (MTS)

    CellTiter 96R AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound (MTS). The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at 490nm.

    Publish: 2011/3/21

  • Cell Proliferation Assay (WST-1)

    The Cell Proliferation Reagent WST-1 is a clear, slightly red, ready-to-use solution, containing WST-1, a tetrazolium salt. By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of 440 nm.

    Publish: 2011/3/21

  • Cell Thawing

    The thawing procedures for regular (non-DMSO-sensitive) cells are performed. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells.

    Publish: 2011/3/11

  • Cell Thawing for DMSO-Sensitive Cells

    This AbVideo describes how to thaw DMSO-sensitive cells. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells.

    Publish: 2011/3/11

  • Chicken Antibody Extraction

    This episode on chicken antibody describes the antibody extraction process. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to Chicken Antibody Extraction.

    Publish: 2014/7/29

  • Chicken Antibody Introduction

    First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than an alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, for it is much more hygienic and cost efficient as well the manufacturing process is more humane. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here is a brief introduction for your first acquaintance of chicken antibody.

    Publish: 2014/7/1

  • Chicken Immune System

    This AbVideo provides in-depth knowledge on the immune system in chickens. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to the chicken immune system.

    Publish: 2014/7/15

  • Chicken Immunization

    Immunization can be a tricky process. This episode on the chicken antibody talks about the variables important for chicken antibody production. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to the immunization of the chicken.

    Publish: 2014/7/22

  • Chicken Myocyte Primary Culture

    Primary culture of chick embryo myocyte culture can be used for the detection and assessment of drug responses on cardiomyocyte.

    Publish: 2014/3/25

  • Clonogenic Assay

    Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate.

    Publish: 2010/9/30

  • CO2 Indicator

    The Fyrite gas analyzer is the most widely used instrument for measuring carbon dioxide (CO2) levels in incubators. It uses the Orsat method of volumetric analysis involving chemical (potassium hydroxide) absorption of CO2 gas.

    Publish: 2011/2/11

  • Coated Plate Preparation

    Plate surface that is coated with poly-D-lysine possesses a uniform net positive charge. This uniform net positive charge is preferred by certain cell types and can subsequently enhance cell attachment, growth and differentiation.

    Publish: 2011/4/19

  • Competent Cell Preparation

    Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. Because DNA is hydrophilic, it will not normally pass through membrane. In order to make these cells readily incorporate foreign DNA, they must first be made "competent" to take up foreign DNA.

    Publish: 2010/7/5

  • Competitive ELISA

    In competitive ELISA, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well. The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.

    Publish: 2010/5/3

  • Covalently Closed Circular DNA (cccDNA) Isolation

    cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.

    Publish: 2011/6/29

  • Cre-Lox Recombination

    Cre-Lox recombination is a special type of site-specific recombination. The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. It is often used in the generation of knockout and conditional knockout animals.

    Publish: 2010/4/12

  • CytoQuest™ CR CTC Automated Leucosep Preparation

    An automated system performing PBMC extraction is shown in this AbVideo. After Leucosep preparation, cells can either be banked or followed by downstream CTC enumeration using CytoQuest™ CR. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/1/13

  • CytoQuest™ CR CTC Capture

    CytoQuest™ CR CTC Capture AbVideo focuses on system operation including processes on parameters and settings, system priming, buffer filling, sample loading and capturing as well as cell fixation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/2/3

  • CytoQuest™ CR CTC Capture and CTC FISH - Dos and Don`ts

    The AbVideo on CytoQuest™ CR Dos and Don’ts part 3 points out few precautions one should pay attentions to when performing capture antibody immobilization, CTC capture and CTC FISH slide preparation.

    Publish: 2015/3/24

  • CytoQuest™ CR CTC Capture Antibody Immobilization

    This AbVideo demonstrates step by step protocol of antibody immobilization on CytoChipNano before CTC capture. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/30

  • CytoQuest™ CR CTC FISH

    For the convenience of researchers, CytoChipNano can be used in fluorescence in situ hybridization (FISH) after immunostaining. Steps on detaching micromixer and applying FISH probes are presented in this AbVideo. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/4/14

  • CytoQuest™ CR CTC Immunostaining

    A thorough description on how to perform immunostaining directly on CytoChipNano after CTC capture is performed by CytoQuest™ CR. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2015/1/26

  • CytoQuest™ CR CTC Leucosep Preparation

    Prior to CTC enumeration by CytoQuest™ CR, blood sample pre-treatment involving cell separation and PBMC extraction from whole blood are necessary. This AbVideo shows detailed Leucosep preparation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/31

  • CytoQuest™ CR CTC Post-Leucosep Freezing and Banking

    This episode of CytoQuest™ CR CTC AbVideo provides procedures on cell freezing and banking after Leucosep preparation. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/30

  • CytoQuest™ CR Plugs Assembly and CytoChipNano Placement - Dos and Don`ts

    The AbVideo on CytoQuest™ CR Dos and Don’ts part 2 concentrates on the proper handling of CytoChipNano and Coupler including the insertion and removal of outlet and inlet plugs into and from the Coupler, and the placement of CytoChipNano in the Chip Receptor.

    Publish: 2015/3/17

  • CytoQuest™ CR SCx™ Spiral Chamber Assembly - Dos and Don`ts

    The AbVideo on CytoQuest™ CR Dos and Don`ts part 1 focuses on the proper procedures of the SCx™ Spiral Chamber Assembling including putting all the necessary parts together for the SCx™ Spiral Chamber, providing detail precautions on how to dock the SCx™ Spiral Chamber onto the Chamber Port of the Coupler and insert the SCx™ Spiral Chamber end tubing into the sample vial.

    Publish: 2015/3/13

  • CytoQuest™ CR System Maintenance

    This AbVideo provides instructions on maintaining CytoQuest™ CR to avoid contaminations and ensure prolong usage. Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

    Publish: 2014/10/30

  • Cytoscape

    Cytoscape is an open source bioinformatics software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data.

    Publish: 2011/1/28

  • DEPC Treatment

    Diethylpyrocarbonate (DEPC) is used in the laboratory to inactivate the RNase enzymes from water and other laboratory utensils. It inactivates the RNases by the covalent modifications of the histidine residues. DEPC treated (and therefore RNase-free) water is used in handling of RNA to reduce the risk of RNA being degraded by RNases.

    Publish: 2011/4/19

  • Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)

    Human umbilical vein endothelial cells (HUVEC) are commonly used as a laboratory model system for the physiological and pharmacological investigations. This video describes how to derive HUVEC from the endothelium of veins of the umbilical cord.

    Publish: 2013/3/29

  • DNA Electrophoresis

    DNA electrophoresis is an analytical technique used to separate DNA fragments based on size, electrical charge and other physical properties. The DNA separates out into bands, with the distance from the electrode corresponding to length of the strand. The technique plays a role in identifying genes for diagnosing disease and for other forms of genetic research.

    Publish: 2010/10/25

  • DNA Extraction by Phenol/Chloroform

    This method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing phenol and chloroform, resulting in an upper aqueous phase and a lower organic phase (mainly chloroform). DNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

  • DNA Extraction from Mouse Tail (Manual)

    DNA extraction is a routine procedure to collect DNA for subsequent molecular analysis. This video shows you how to extract DNA from mouse tails. There are three basic steps: (i) break cells by lysis buffer, (ii) digest protein and remove contamination by proteinase, (iii) precipitating the DNA.

    Publish: 2010/8/30

  • DNA Gel Preparation

    This AbVideo demonstrates step by step of the agarose gel preparation for DNA electrophoresis. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.

    Publish: 2010/10/4

  • DNA Sequencing

    DNA sequencing is a technique to determine the order of the nucleotide bases-adenine, guanine, cytosine, and thymine. It is indispensable for basic biological research and discovery.

    Publish: 2010/1/14

  • Dot Blot

    Dot blot is a technique can be used as a semiqualitative method for rapid screening of a large number of samples. It is for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane.

    Publish: 2010/2/12

  • EHCO - Encyclopedia of Hepatocellular Carcinoma Genes Online

    EHCO (Encyclopedia of Hepatocellular Carcinoma genes Online) is an integrative platform to systematically collect, organize and compare the pileup of unsorted HCC-related studies by using natural language processing and softbots.

    Publish: 2011/1/14

  • Electrical Safety Test

    Electrical safety testing is pivotal for electrical products to conform to safety standards promulgated by safety and standard agencies such as UL, CE, VDE, CSA, BSI, CCC, etc. Here we present a demonstrative operation of electrical safety compliance analyzer, ESA-140, EXTECH Electronics Co., Ltd. The topics tested here include dielectric voltage-withstand test and earth bond test. The guidelines of these tests are majorly described in IEC 60335, IEC 61010 and many other national and international standards.

    Publish: 2014/5/27

  • ELISA Substrate Kit - Introduction

    ELISA Substrate Kit is a series of extremely sensitive chemiluminescent substrate kit for detecting horseradish peroxidase (HRP) conjugates in ELISA. Its very intense signal output at 425 nm enables detection of low femtogram amounts of antigen. Here is a brief introduction to why you should choose our products for your ELISA assay.

    Publish: 2014/9/2

  • Embryoid Body Formation - Ultra-Low Attachment Dishes

    Embryoid Bodies (EB) Formation is an in vitro method to evaluate the pluripotency of the cells. EBs are formed in ultra low attach dishes that promote the aggregation of cells.

    Publish: 2012/6/7

  • Emulsification (CFA and IFA)

    Emulsification is a process to mix adjuvants and immunogen. Adjuvants are mixed and injected with an antigen to prevent catabolism and help increase the immune response by localizing the antigen for an extended time and attracting the appropriate cells (T cells, B cells and APC) to interact with it.

    Publish: 2010/8/26

  • EtBr Handling

    Ethidium Bromide (EtBr) is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis. It is a potent mutagen, so its hazardous properties require special safe handling and disposal procedures.

    Publish: 2011/4/19

  • FASTA Formatted Sequence Record

    FASTA format is a text-based format for representing either nucleotide sequences or peptide sequences, in which base pairs or amino acids are represented using single-letter codes. The simplicity of FASTA format makes it easy to manipulate and parse sequences using text-processing tools (e.g. BLAST).

    Publish: 2010/12/13

  • FastLink R-PE Labeling Kit

    The FastLink R-PE Labeling Kit allows PE conjugations to set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing PE. By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, FastLink technology can be used to label small quantities of protein for FACS analysis with 100% recovery. This video shows the procedure for setting up conjugation reactions.

    Publish: 2011/6/29

  • Flow Cytometry - Cell Cycle

    This AbVideo focuses on the flow cytometry analysis for a population of cell distribution at different cell cycle stages. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus.

    Publish: 2011/8/2

  • Flow Cytometry - Double Staining

    Step by step of double staining for flow cytometry analysis of cells is presented. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus.

    Publish: 2011/8/2

  • Flow Cytometry - Single Staining

    This AbVideo exemplifies the procedure of single staining for flow cytometry analysis of cells. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus.

    Publish: 2011/8/2

  • Fluid Thioglycollate Medium Preparation

    Fluid Thioglycollate Medium (FTM) is a multi-purpose enriched differentiating media designed to test the aerotolerance of a wide range of bacteria. This video presents the process for preparation of FTM.

    Publish: 2011/8/2

  • Fluorescence In Situ Hybridization (FISH)

    FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. You can use FISH probes for the detection of gene amplification, loss and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library.

    Publish: 2011/5/5

  • Fluorescent Dye - Differential Gel Electrophoresis

    This episode of fluorescent dye discusses the theory behind differential gel electrophoresis (DGE) and the advantages of using Abnova’s DGE labeling kit. As an alternative application, 2D-PAGE electrophoresis represents a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. Abnova presents a series of DGE labeling kits for faster, easier and more accurate quantification in 2D-PAGE. Please visit the webpage "DGE Labeling Kit" for more information. Here is a brief introduction to why you should choose our products for your fluorescence labeling.

    Publish: 2014/8/19

  • Fluorescent Dye - Introduction

    As a novel product line, Abnova provides a complete selection of fluorescent labels covering the full working range of wavelengths, from 400nm to 850nm. The structures of our dyes are designed so as to provide great brightness and photo-stability, for high quality molecular imaging. Each fluorescent compound is available with many different reactive moieties, while retaining high photophysical features and different charge values (anionic, cationic, zwitterionic and neutral). Please visit the webpage "Fluorescent Dyes" for more information. Here we present a brief introduction to our fluorescence dye system.

    Publish: 2014/8/5

  • Fluorescent Dye - Protein Labeling

    After an introduction on Abnova’s fluorescent dye, this AbVideo shows you how easily it is to utilize Abnova’s kit to perform antibody labeling. As a novel product line, Abnova provides a complete selection of fluorescent labels covering the full working range of wavelengths, from 400nm to 850nm. The structures of our dyes are designed so as to provide great brightness and photo-stability, for high quality molecular imaging. As a more convenient and efficient way for fluorescent protein labeling, Abnova also provides our series of fluorescent dyes in kit format. Please visit the webpage "Fluorescent Dyes" for more information. Here is a brief introduction to why you should choose our products for your fluorescence labeling.

    Publish: 2014/8/12

  • Freezing of Adherent Cells

    This AbVideo illustrates the process of adherent cells freezing. Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. The cryopreservation agent and condition can depend on cell type.

    Publish: 2011/3/11

  • FSH ELISA Kit

    FSH ELISA Kit is a solid-phase enzyme immunoassay for the quantitative detection of FSH. This video shows the procedures of the kit.

    Publish: 2014/4/8

  • Gelatin Preparation and Coating

    Gelatin solution is used to coat 6-well plates for culture of Mouse Embryonic Fibroblasts (MEFs). MEFs may be used as feeder cell layer to support the survival and growth of human embryonic stem cells (hESCs). This video shows the steps for preparation of gelatin solution and gelatin-coated plates.

    Publish: 2011/8/2

  • Gene Conserved Domains

    Conserved domains database (CDD) is a protein annotation resource that consists of a collection of well-annotated multiple sequence alignment models for ancient domains and full-length proteins. These are available as position-specific score matrices (PSSMs) for fast identification of conserved domains in protein sequences via RPS-BLAST (Reverse Position-Specific).

    Publish: 2010/7/29

  • Gene Homology

    HomoloGene database identifies homologs among the annotated genes of more than a dozen completely sequenced eukaryotic genomes using an automated procedure. It can find gene homologs (paralogs, orthologs, homologs) based on protein sequence similarity and access pre-computed multiple alignments of homologous proteins.

    Publish: 2010/8/19

  • Gene Intron-Exon

    The Reference Sequence (RefSeq) database is a non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa. RefSeq also reports the information about exon and intron boundaries and length.

    Publish: 2010/10/21

  • Gene Map Viewer

    Map Viewer allows you to view and search complete genome of an organism, display chromosome maps, and zoom into progressively greater levels of detail, down to the sequence data for a region of interest.

    Publish: 2010/8/19

  • Gene ORF Finder

    ORF Finder identifies all possible ORFs in a DNA sequence by locating the standard and alternative stop and start codons. The deduced amino acid sequences can then be used to BLAST against GenBank.

    Publish: 2010/10/14

  • Gene Pathway

    Gene pathway represents molecular pathways for metabolism, genetic information processing, environmental information processing, other cellular processes, human diseases, and drug development.

    Publish: 2010/8/12

  • Gene SNP

    dbSNP resource serves both as a repository for genomic variation data (including single nucleotide polymorphisms, microsatellites and small insertion/deletion mutations) and as a computational analysis resource. dbSNP data will provide the researcher with extensive data and information about variations, evolution, disease and more.

    Publish: 2010/8/12

  • GeneCards®

    GeneCards® is an integrated database of human genes that includes automatically-mined genomic, proteomic and transcriptomic information, as well as orthologies, disease relationships, SNPs, gene expression, gene function, and service links for ordering assays and antibodies.

    Publish: 2011/1/28

  • GEO DataSets

    The Gene Expression Omnibus (GEO) is a public repository that stores original submitter-supplied curated gene expression DataSets. This video shows you how to enter search terms to locate experiments of interest and interpret GEO DataSets results pages.

    Publish: 2011/11/2

  • GST-tagged Protein Purification

    Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. We use GST-fusion protein to purify and detect proteins of interest. The GST-fusion protein can be purified from cells via its high affinity for glutathione.

    Publish: 2010/2/8

  • H&E Staining

    H&E stain is a popular staining method in histology. Its a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you will usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink).

    Publish: 2010/5/17

  • Handling Adherent Cells upon Receipt

    Handling of adhering cell is the key point before culturing. This video shows you how to disinfect the flask well, confirm the condition of the cells under microscopy, and re-attach the cell that may have detached during transportation.

    Publish: 2011/5/27

  • Handling Suspension Cells upon Receipt

    Handling of suspending cell is the key point before culturing. This video shows you how to disinfect the tube well, transfer the cell suspension to a flask or dish, and make sure the condition of the cells under microscopy.

    Publish: 2011/5/27

  • HCG ELISA Kit

    HCG ELISA Kit is a solid-phase enzyme immunoassay for the quantitative detection of hCG. This video shows the procedures of the kit.

    Publish: 2014/4/29

  • Hematology Test

    As a mature diagnostic tool, regular blood test is widely adopted to evaluate anemia, leukemia, reaction to inflammation and infections, etc. With automated hematology analyzer, the test result of CBC with DIFF (Complete Blood Count with Differential) can easily be obtained. The test collects information including the number and types of different types of blood cells, the variation in the size of red blood cells, hematocrit, hemoglobin value, platelet count, mean corpuscular hemoglobin, and the average size of red blood cells. Here we demonstrate a simple operation of hematology test for following clinical diagnosis.

    Publish: 2014/6/17

  • hESCs Cryopreservation

    Human embryonic stem cells (hESCs) are pluripotent cells able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. This video describes how to prepare cryopreserved hESCs for long-term storage.

    Publish: 2012/6/7

  • Human Protein Reference Database

    The Human Protein Reference Database represents a centralized platform to visually depict and integrate information pertaining to domain architecture, post-translational modifications, interaction networks and disease association for each protein in the human proteome.

    Publish: 2011/1/28

  • IHC Kit - RealBlue Peroxidase Substrate Kit

    RealBlue Peroxidase Substrate kit is 50-100 times more sensitive than DAB and is non-carcinogenic. Strongly recommended for the detection of least-abundant markers. RealBlue forms a blue chromogenic product, providing excellent contrast with DAB and other substrates for multiple labeling experiments

    Publish: 2010/12/10

  • Immunofluorescence

    Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope.

    Publish: 2009/12/13

  • Immunoprecipitation

    Immunoprecipitation (IP) is the technique of precipitating an protein antigen out of solution using an antibody specific to that antigen. This antibody is immobilized on a solid-phase substrate such as protein A/G agarose beads. The beads are then added to the protein mixture and the proteins that are targeted by the antibodies are captured onto the beads.

    Publish: 2011/4/19

  • Indirect ELISA

    The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies.

    Publish: 2010/4/26

  • Insect Cell Culture

    The Sf9 cell line derived from pupal ovarian tissue of the Fall armyworm Spodoptera frugiperda is one of the most common cell lines used for BEVS (Baculovirus expression vector systems). This video shows you how to culture the Sf9 cell line.

    Publish: 2010/5/31

  • Integra CL350 Cell Cultivation

    The CELLine bioreactor is a disposable, two-compartment cultivation device suitable for many cell culture applications. There are two sizes of the CELLine, CL350 and CL 1000. We use CL350 to produce monoclonal antibodies on a laboratory scale.

    Publish: 2010/6/30

  • Invasion Assay

    Cell invasion assay is designed to accelerate the screening process for compounds that influence cell migration through extracellular matrices. Matrigel (BD), extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, resembles the extracellular matrices is used by cell biologists as a substrate in cell invasion assay.

    Publish: 2010/11/12

  • Isotope Labeling

    Isotopic labeling is a technique for labeling a substance with a stable or radioactive isotope. By measuring the radioactivity or the abundance of the isotope, we could made observations of the course through a biologic process. This video shows the procedure for using radioisotope phosphorus-32 to label DNA.

    Publish: 2011/7/1

  • LB Medium Preparation

    LB broth is used for maintaining and cultivating recombinant strains of Escherichia coli. The ingredients of LB broth are tryptone, yeast extract and Sodium Chloride. We show you how to prepare the LB medium.

    Publish: 2010/7/22

  • LB Plate Preparation

    An agar plate is a Petri dish that contains a growth medium (typically agar plus nutrients) used to culture microorganisms. We show you how to prepare the LB agar plate.

    Publish: 2010/7/22

  • Lentiviral Infection

    Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector.

    Publish: 2011/7/1

  • Lentiviral Production

    Lentiviruses are able to deliver genes into almost any mammalian cell type and have the ability to replicate in non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. This video presents the process of lentiviral production in 6cm plate.

    Publish: 2011/3/21

  • Lentivirus RIU Test

    This video presents the protocol for relative titer method for lentivirus. Lentivirus titers are estimated as relative infectious units (RIU), according to the curve of cell viability versus viral dose.

    Publish: 2011/8/2

  • LEP (Human) Clinical Range ELISA Kit

    LEP (Human) Clinical Range ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human leptin in serum, plasma and tissue culture medium. This video shows the procedures of the kit.

    Publish: 2011/9/26

  • Luciferase Assay

    Firefly luciferase is widely used as a reporter to study gene expression and other cellular events. The Luciferase Assay is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Through the assay, light is produced by converting the chemical energy of luciferin oxidation and can be measured by a luminometer.

    Publish: 2011/3/21

  • Lyophilization

    Biological materials often are dried to stabilize them for storage or distribution. Lyophilization also called freeze-drying, is one method of drying biological materials that minimizes damage to its internal structure.

    Publish: 2010/6/30

  • MaxBead Carboxyl Labeling Kit Operation

    MaxBead Carboxyl Labeling Kit provides whole set of materials for researchers to bind the desired ligand onto magnetic beads conveniently. If only the ligand contain primary amine groups such as antibody, protein, or peptide, it can be conjugated to the magnetic beads easily.

    Publish: 2014/5/13

  • MaxBead Protein A/G Operation

    MaxBead Protein A/G is designed as a rapid and simple tool for immunoprecipitation, purification/depletion assays, and other magnetic separation applications. Antibody can easily bind to the magnetic beads due to its high affinity with protein A/G.

    Publish: 2014/5/20

  • Methylation-specific PCR

    Methylation-specific PCR (MSP) is a rapid and inexpensive method that can be used to determine the methylation status of DNA. It is a bisulfite conversion based PCR technique. In MSP, two primer pairs are designed for methylated and unmethylated DNA.

    Publish: 2010/8/5

  • Micro-Manipulator - Probe Preparation

    Precise physical interactions under microscopic level turn out to be reachable because of the aid from micromanipulator. In concert with hydraulic drive systems, it is principally the glass probe that makes the physical contact with target cell for purposes like single cell collection, injection, patch clamping, etc. Here we present you step by step of probe preparation including how to do glass capillary pulling and micropipette tip processing. Instruments adopted here are MF-900 microforge and PC-10 puller, both from NARISHIGE Group.

    Publish: 2014/4/15

  • Micro-Manipulator - Single Cell Collection

    With the aid of micromanipulator, precise physical interactions, like single cell collection, injection, patch clamping, etc. under microscopic level becomes achievable. In general, depending on the application, one or more micromanipulators can be docked on a microscope stage to operate in microscopes field of view. A typical method for single cell collection is displayed including steps of cell aspiration and discharge. And, MMO-203 Three-axis Oil Hydraulic Micromanipulator from NARISHIGE Group is adopted here.

    Publish: 2014/4/22

  • Micropipette Calibration

    Micropipettes are used to accurately measure and dispense small volumes of liquid. The capacity of a micropipette can range from less than 1μl to 1000μl (1ml). To ensure that obtained results using micropipette are reliable, it is necessary to calibrate micropipettes several times a year. This video shows a method for testing the accuracy of automatic micropipettes.

    Publish: 2010/7/19

  • Micropipette Guideline

    Pipetman is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation. This video provides some pipetting guidelines and precautions.

    Publish: 2010/10/21

  • Microwestern Arrays - Lecture Series 1

    Lecture series 1 compares different array technologies and reveals their limitations and current challenges which brings out the importance of microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 2

    Lecture series 2 explicitly explains the step-by-step guide of performing microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 3

    Lecture series 3 demonstrates Dr. Chuu’s microwestern array experiments in details and also explains his results using different analyses. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 4

    Lecture series 4 points out the future direction and potential application using microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Migration Assay

    The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted.

    Publish: 2010/11/4

  • Mononuclear Cell Isolation from Whole Blood

    This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps.

    Publish: 2010/5/31

  • mRNA Extraction

    Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

    Publish: 2010/4/19

  • Mycoplasma Assay

    Mycoplasma is highly infectious and cross-contamination commonly occurs when new cells are introduced into laboratories. We use PCR-based method for detection of Mycoplasma weekly. It is a highly sensitive, specific and rapid method to detect mycoplasma contamination in cell cultures.

    Publish: 2010/3/22

  • Mycoplasma Detection - Direct Method

    “Direct method” for detecting Mycoplasma in the cell samples is provided. Using microtest to determine if mycoplasmal contaminant is present stands one of the most significant cell culture procedures.

    Publish: 2011/8/8

  • Mycoplasma Detection - Hoechst DNA Staining

    This AbVideo tells you how to detect Mycoplasma in vero cell samples through the “Hoechst DNA staining method”, since one of the most important cell culture procedures is to use microtest to identify whether mycoplasmal contaminant is present or not.

    Publish: 2011/8/8

  • Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)

    Mycoplasma is a common and serious contamination of cell cultures. Mycoplasma contamination can cause the alteration of the phenotypic cell characteristics. PCR method allows for fast and reliable identification of mycoplasma contamination in cell cultures.

    Publish: 2013/4/8

  • Mycoplasma Detection Agar Preparation

    Mycoplasma detection agar (without crystal violet) is used with enrichments for the identification and cultivation of Mycoplasma. This video demonstrates the steps to prepare Mycoplasma agar.

    Publish: 2011/12/2

  • Mycoplasma Detection Broth Preparation

    Mycoplasma detection broth (without crystal violet) is used for the isolation and cultivation of Mycoplasma for research studies. This video shows you how to prepare the Mycoplasma broth.

    Publish: 2011/12/2

  • Nuclear and Cytoplasmic Protein Extraction

    Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells.

    Publish: 2011/4/19

  • Oil Red O Staining for Adipogenesis

    Oil Red O is a lysochrome diazo dye used for demonstrating neutral triglycerides and lipids in tissue. This video shows the procedure of Adipogenesis staining with Oil Red O.

    Publish: 2013/4/8

  • OMIM Database

    OMIM (Online Mendelian Inheritance in Man) is a catalog of human genes and genetic disorders, with links to literature references, sequence records, maps, and related databases. It is based on the book, Mendelian Inheritance in Man. The online version is updated daily.

    Publish: 2010/10/25

  • Optical Fiber Cleavage

    This AbVideo demonstrates the operation of automated fiber cleavage to produce flat and angled cleaves on fibers ranging from 80 microns to 1.25 mm in diameter for following biological applications. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/3

  • Optical Fiber End-Face Analysis

    This AbVideo demonstrates the operation of automated interferometric inspection system specifically designed for checking the surface quality and flatness of cleaved, polished or lensed fibers. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/10

  • Particle Blot for HBV Particle

    Hepatitis B viruses (HBV) are double-stranded DNA viruses that cause acute and chronic hepatitis. Furthermore, HBV infection is the leading cause of liver cancer. This video shows the procedure for using particle blot to analyze HBV Particle.

    Publish: 2011/6/17

  • Patau Syndrome

    Patau Syndrome, resulted from chromosome 13 trisomy, is regarded the least common but the most severe among those somatic aneuploidies. It is typically associated with significant infant mortality. Clinical features of Patau syndrome include delayed development with central nervous system anomalies, midline facial defects, and urogenital malformations. Medical management of children with Trisomy 13 should be planned on a case-by-case basis according to the individual circumstances of the patient. Here we present a brief introduction to the common knowledge of Patau Syndrome.

    Publish: 2014/10/1

  • PCR Cloning

    PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid.

    Publish: 2010/3/31

  • PCR Cloning (I) - Inserted Fragment Preparation

    The procedure of PCR cloning includes DNA preparation, digestion, ligation and transformation. This video shows the first part- insert PCR. Compared to other Taq-generated PCR inserts, Pfu DNA polymerase-generated PCR fragments will have fewer errors and is more commonly used for molecular cloning.

    Publish: 2010/3/22

  • PCR Cloning (II) - Digestion

    Digestion is the process of cutting DNA molecules with restriction endonucleases. These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA. In PCR cloning, insert and vector are cutted by the same restriction endonucleases and then join together in the next step, ligation.

    Publish: 2010/3/29

  • PCR Cloning (IV) - Transformation

    The purpose of transformation is to introduce a foreign plasmid into bacteria and the bacteria will replicate the foreign plasmid along with their own DNA. Bacteria which are able to uptake DNA after a heat shock are called "competent".

    Publish: 2010/3/29

  • PCR Cloning (V) - Colony Selection

    It can be used after a transformation to screen colonies for the plasmid. Primers designed for the insert sequence should be used when preparing the PCR reaction. Thus, any colonies which give rise to an amplification product are likely to contain the correct DNA sequence.

    Publish: 2010/3/29

  • PCR Purification

    Following PCR, you need to get rid of excess short primers, dNTPs, enzymes, short-failed PCR products and salts. We use a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O to get clean PCR products for downstream applications.

    Publish: 2010/3/22

  • pH-meter Calibration

    pH meter is an electronic instrument used to measure the pH of a liquid. For very precise work the pH meter should be calibrated before each measurement. Calibration is performed with three standard buffer solutions (pH4.0, pH7.0, pH10.0) that span the range of pH values to be measured.

    Publish: 2010/7/19

  • Plaque Assay for Influenza Virus

    The plaque assay is a widely used approach for purifying a clonal population of virus and determining the viral titers (the lowest concentration of virus that results in infection). This video shows the procedure of plaque assay for influenza virus.

    Publish: 2013/4/8

  • Plasmid DNA Extraction (CsCl Isolation)

    CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method.

    Publish: 2011/8/30

  • Plasmid DNA Extraction (Gigaprep)

    Gigaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 2.5~5 L of LB broth and the expected DNA yield is 7.5~10 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes megaprep, midiprep, and miniprep.

    Publish: 2010/5/24

  • Plasmid DNA Extraction (Megaprep)

    Megaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 500 ml~2.5 L of LB broth and the expected DNA yield is 1.5~2.5 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, midiprep, and miniprep.

    Publish: 2010/5/24

  • Plasmid DNA Extraction (Midiprep)

    Midiprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 15-25 ml of LB broth and the expected DNA yield is 100-350 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and miniprep.

    Publish: 2010/5/17

  • Plasmid DNA Extraction (Miniprep)

    Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and midiprep

    Publish: 2010/4/26

  • Plasmid DNA Extraction (Traditional Alkaline Lysis Method)

    Alkaline lysis is one of the most generally useful methods for isolating circular plasmid DNA from bacterial cells. This video demonstrates the procedures for alkaline lysis method.

    Publish: 2011/8/2

  • POINeT - Protein Interactome Datasets

    Protein-protein interactions (PPIs) are critical to every aspect of biological processes. Even though a number of software tools are available to facilitate PPI network analysis, an integrated tool is crucial to alleviate the burden on querying across multiple web servers and software tools. POINeT, an integrated web service, have been constructed to simplify the process of PPI searching, analysis, and visualization.

    Publish: 2011/1/6

  • Polymerase Chain Reaction

    Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles.

    Publish: 2010/1/7

  • Pork (Pig) ELISA Kit

    Pork (Pig) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative detection of pork in food. This video shows the procedures of the kit.

    Publish: 2014/5/6

  • Prenatal Tests for Genetic Disorders

    To identify birth defects such as Down syndrome, chromosome abnormalities, and other genetic diseases, prenatal test is now widely accepted as a routine for pregnant women. Common tests including amniocentesis, ultrasonography, etc. can be basically divided into invasive or non-invasive methods according to the way of their appliance. An invasive method involves probes or needles being inserted into the uterus, and , on the contrary, non-invasive techniques adopt ultrasonography and maternal serum screens to gain the information of the fetus. Here we present a brief introduction to the most common kinds of prenatal tests along with some basic knowledge of genetic consultation.

    Publish: 2014/9/9

  • Propidium Iodide Staining

    Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule that can be used to stain DNA. PI is used as a DNA stain for both flow cytometry to evaluate cell viability or DNA content in cell cycle analysis and microscopy to visualize the nucleus and other DNA containing organelles. It can be used to differentiate necrotic, apoptotic and normal cells.

    Publish: 2010/12/10

  • Protein Quantification (BCA Assay)

    The bicinchoninic acid assay (BCA Assay) is a biochemical assay for determining the total level of protein in a solution, similar to Bradford protein assay. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

    Publish: 2010/5/24

  • Protein Quantification (Bradford Assay)

    Bradford assay is a rapid and accurate method to determine the concentration of protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mg/ml), the more protein present, the more Coomassie binds.

    Publish: 2010/3/15

  • Protein Quantification (CBR)

    Coomassie Blue R (CBR) is sensitive for protein detection in PAGE gels, and protein can be directly quantitated by colorimetric. Coomassie Blue dye binds to peptides in an acidic medium. The color change of this assay is measured at 595 or 600 nm. We calculate the protein concentration using the formula created by standards. This AbVideo displays the procedure of protein quantification by using CBR.

    Publish: 2010/2/8

  • Protein Structure

    "Entrez Structure", also known as Molecular Modeling DataBase (MMDB), is a database of experimentally determined structures obtained from the RCSB Protein Data Bank (PDB). It provides a wealth of information on the biological function, on mechanisms linked to the function, and on the evolutionary history of relationships between macromolecules.

    Publish: 2010/9/30

  • Protemist® DT - Automated Wheat Germ Protein Synthesizer

    Automated protein synthesizer is an expert system in transcription, translation, and purification of recombinant protein. It is based on the eukaryotic translation apparatus of wheat germ. This high throughput system produces recombinant proteins in vitro. The generated proteins display foldings and biological functions similar to the mammalian counterparts.

    Publish: 2010/1/18

  • Protemist® DT II and XE - Demonstration

    The demonstration for Protemist® DT II - High Throughput Protein Expression and Protemist® XE - Large-Scale Protein Expression.

    Publish: 2010/9/24

  • Protemist® XE - Large-Scale Protein Expression

    Protemist® XE is a fully-automated desktop protein synthesizer for large scale protein production using the wheat germ cell-free expression system. It is ideally suited for producing 10 mg to over 500 mg of protein for structural analysis, functional analysis, and assay development.

    Publish: 2010/8/10

  • PubMed Literature Database

    PubMed is a free literature database which comprises more than 20 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

    Publish: 2010/10/25

  • Radioactive Signal Detection

    FUJIFILM FLA-2000 is a multiple imaging application suitable for fluorescence and radioisotopic measurement. Laser scanning with the FLA-2000, instead of using conventional photography, permits analysis and digital storage. With FUJIFILM Imaging Plate (IP), a technology which is 100 times more sensitive to radiation than X-ray film, exposure times are reduced to 1/10 - 1/20 of film and amount of radioisotope is reduced.

    Publish: 2011/3/21

  • Raw Cell Transmigration

    The ability of cell migration is commonly used for the evaluation of cell invasion or wound repair. Using boyden chamber is convenient for the assessment of cells responses toward genetic manipulation or chemo attraction treatment.

    Publish: 2014/3/17

  • RNA Electrophoresis

    RNA electrophoresis is an analytical technique used to separate RNA fragments. RNA has the tendency to form both secondary and tertiary structures that can impede its separation. So a denaturing condition is performed in formaldehyde and MOPS buffer system. RNA under this condition is fully denatured and migrates according to its molecular weight.

    Publish: 2011/2/16

  • RNA Extraction by TRIzol®

    TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

  • RNA Extraction from Cell Culture

    Extracting RNA from cultured cells has traditionally been a highly repetitive method, and this AbVideo indicates the steps on how to do it. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2010/4/19

  • RNA Extraction from Tissue

    Difficulties of isolating intact total RNA from tissue samples vary with the physical and biochemical nature of tissue. This AbVideo demonstrates how we can extract total RNA from tissue. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2010/4/19

  • RNA Gel Preparation

    The quality of RNA preparation could be measured by electrophoresis on a denaturing agarose gel. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the formaldehyde agarose gel for the electrophoresis of RNA.

    Publish: 2011/1/6

  • Rotary Microtome Section

    Rotary microtome is an instrument used to cut the blocks of tissue embedded in paraffin into micro fine slices (known as sections). Microtome is an important device in microscopy preparation, allowing for the preparation of samples for observation under transmitted light or electron radiation.

    Publish: 2010/6/21

  • Safranin Staining for Chondrogenesis

    Safranin is a biological stain used for the detection of cartilage, mucin and mast cell granules. This video shows how to stain chondrogenesis with safranin.

    Publish: 2013/4/8

  • Sandwich ELISA

    The Sandwich ELISA measures the amount of analyte between capture antibody and detection antibody. The analyte needs to have two different epitope sites available for antibody binding.

    Publish: 2010/2/12

  • Sandwich ELISA - Isotype Detection

    Sandwich ELISA is performed to determine the amount and serological class of antibodies made by an immunized animal or present in the serum of patients. The anti-immunoglobulin antibodies used have high specificity and sensitivity.

    Publish: 2010/6/30

  • Start-up and Clean-up Procedures for Flow Cytometer

    There are several steps after turn on and before turn off the flow cytometer. These steps are important to ensure the accuracy and reliability of results and consistent instrument functioning.

    Publish: 2011/8/2

  • Stem Cell Embryoid Body Formation

    This video shows a novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel. [Reference: Biomacromolecules. 2007 Sep;8(9):2746-52]

    Publish: 2010/10/21

  • Subculture of Adherent Cells

    Growing cell cultures requires subculturing (or passaging) every few days to avoid overcrowding and to increase the number of cell samples for research use. This video teaches you how to subculture adherent cells.

    Publish: 2011/2/25

  • Subculture of Suspension Cells

    This AbVideo demonstrates how to subculture the suspension cells since passaging the cells is a crucial step for growing cell cultures to avoid overcrowding. Another good reason for subculturing is to gain more cell samples for research use.

    Publish: 2011/2/25

  • Terrific Broth (TB) Preparation

    Terrific Broth (TB) is an enriched medium used with glycerol in cultivating Escherichia coli. TB allows the bacteria to produce a greater number of recombinant proteins and plasmid yield. This video shows you how to prepare TB.

    Publish: 2011/7/1

  • Thawing Inactivated Feeder Cells (MEFs)

    Mouse embryonic fibroblasts (MEFs) have been used as feeder cell layers for the culture of Human Embryonic Stem (hES) cells. MEFs could promote optimum growth and prevent differentiation of hESCs. This video describes how to thaw MEF cells and seed in plate for usage.

    Publish: 2011/12/2

  • The Advantages of Chicken Antibody

    The advantages on using chicken antibody are well discussed in this AbVideo. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to the advatages of chicken antibody.

    Publish: 2014/7/8

  • Tissue Grinder

    The tissue grinder provides rapid and efficient disruption of up to 12 biological samples, including animal and human tissues, plant tissues, bacteria, and yeast. Disruption and homogenization are achieved through the beating and grinding effect of beads on the sample material as they are shaken together in 2 ml sample tubes.

    Publish: 2010/11/26

  • Tissue Lysate Preparation (Native)

    Tissue lysate preparation is explained in this protocol which exemplifies the procedure with three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.

    Publish: 2010/6/7

  • Tissue Microarray

    Tissue Microarray is a powerful new technology for high throughput analysis of protein expression in a large number of tissue samples. Hundreds of tissue cores are arranged on a single slide, and then analyzed by immunohistochemistry staining. We offer a large collection of immmunohistochmistry (IHC) validated antibodies for the tissue microarray platform.

    Publish: 2009/12/8

  • Total IgG (Human) ELISA Kit

    Total IgG (Human) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of total human IgG in human serum and plasma. This video shows the procedures of the kit.

    Publish: 2014/4/1

  • Urinalysis

    Urinalysis is one of the most common methods in medical diagnosis. It includes an array of tests performed with urine, such as the concentration of ions, trace metals, protein and other molecules. In principle, the test result of test strips can be read as color changes. With automated urine analyzer, artifacts of data judgment can be further avoided. Here is a brief demonstration of regular urine test using urine test strip in concert with automated analyzer.

    Publish: 2014/6/24

  • UV-Vis Spectrophotometer - Nanodrop®

    The Thermo Scientific Nanodrop® is a full-spectrum spectrophotometer that measures 1 ul samples with high accuracy and reproducibility. It utilizes a sample retention technology that eliminates the need for cumbersome cuvettes and allows for clean up in seconds.

    Publish: 2010/10/11

  • Volume Estimation in Pipette Tips

    It is important to get a feel for the appearance of volume levels of pipette tips. We show you how to recognize volumes in pipette tips .It may help you catch a mistake before it happens.

    Publish: 2010/8/5

  • Website Tutorial - Product Comparison

    This tutorial teaches you how to compare products on Abnova website. You can choose at least 2 products (up to 10) to compare. It shows specifications of each product. Then choose the best product for your research.

    Publish: 2010/9/23

  • Western Blot - Ponceau S Staining

    Ponceau S is a rapid and reversible staining method for locating protein bands on Western blots producing reddish pink stained bands. The stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and can be completely removed from the protein bands by continued washing.

    Publish: 2010/11/26

  • Western Blot - SNAP id™ System

    SNAP i.d.™ System is a fast and convenient method for the detection of immunoreactive proteins on western blots. The amount of time required for immunodetection is greatly reduced in 30 minutes with this unique vacuum-driven system.

    Publish: 2010/9/27

  • Western Blot Substrate Kit - Introduction

    ( http://www.abnova.com ) - WB substrate kit detection reagents are non-isotopic, luminol-based chemiluminescence substrate, designed for the chemiluminescent detection of immobilized proteins and immobilized nucleic acids conjugated with horseradish peroxidase (HRP). The peroxidase-catalyzed oxidation of luminol produces a weak flash of light at 425 nm. The incorporation of an electron transfer mediator into the buffer forces the flash signal into a glow and greatly improves the analytical characteristics of the reaction in terms of increased signal intensity and duration. Recent works have shown that, by addition of a suitable acylation catalyst, a further large increase in light output is observed. Here is a brief introduction to why you should choose our products for your fluorescence blotting. More videos at Abnova http://www.abnova.com

    Publish: 2014/8/26

  • Wheat Germ Cell-Free Protein Synthesis - Lecture Series 1

    Lecture series 1 provides an overview on the cell-free protein expression system including the advantages and the procedures involved. Dr. Masaki Madono introduced wheat germ cell-free protein synthesis system. This system is pioneered by Professor Yaeta Endo of Ehime University, Japan. This platform is based on the eukaryotic translation apparatus of wheat germ, which has significant advantages over other commonly used protein expression systems.

    Publish: 2010/9/24

  • Wheat Germ Cell-Free Protein Synthesis - Lecture Series 2

    Lecture series 2 discusses particularly on the bilayer translation, protein synthesis and folding mechanism. Dr. Masaki Madono introduced wheat germ cell-free protein synthesis system. This system is pioneered by Professor Yaeta Endo of Ehime University, Japan. This platform is based on the eukaryotic translation apparatus of wheat germ, which has significant advantages over other commonly used protein expression systems.

    Publish: 2010/9/24

  • Wheat Germ Cell-Free Protein Synthesis - Lecture Series 3

    Lecture series 3 focuses on the robotic protein synthesizers commonly used. Dr. Masaki Madono introduced wheat germ cell-free protein synthesis system. This system is pioneered by Professor Yaeta Endo of Ehime University, Japan. This platform is based on the eukaryotic translation apparatus of wheat germ, which has significant advantages over other commonly used protein expression systems.

    Publish: 2010/9/24

  • Wheat Germ Cell-Free Protein Synthesis - Lecture Series 4

    Lecture series 4 shows the reagents, kits and products that are essential for protein synthesis. Dr. Masaki Madono introduced wheat germ cell-free protein synthesis system. This system is pioneered by Professor Yaeta Endo of Ehime University, Japan. This platform is based on the eukaryotic translation apparatus of wheat germ, which has significant advantages over other commonly used protein expression systems.

    Publish: 2010/9/24

  • Wheat Germ Cell-Free Protein Synthesis - Lecture Series 5

    Lecture series 5 explicitly presents the applications can be provided by the protein synthesis system. Dr. Masaki Madono introduced wheat germ cell-free protein synthesis system. This system is pioneered by Professor Yaeta Endo of Ehime University, Japan. This platform is based on the eukaryotic translation apparatus of wheat germ, which has significant advantages over other commonly used protein expression systems.

    Publish: 2010/9/24

  • Wright and Giemsa Staining

    Wright and Giemsa stains are used to stain peripheral blood and bone marrow smears. The components are oxidized eosin Y, methylene blue, and azure B. They stain the cytoplasm of cells an orange to pink color and nucleus a blue to purple. Wright and Giemsa stains are used to study blood cell morphology.

    Publish: 2010/12/31

  • XYY Syndrome

    XYY Syndrome is a sex chromosomal anueploidy caused by an Y chromosome in male individuals and thus resulted 47,XXY karyotype. In general XXY syndrome is regarded asymptomatic with certain cases reported to be at greater risk for behavioral problems, mild learning disability, delayed speech and language development, along with tall stature. Here we present a brief introduction to the common knowledge of XXY Syndrome.

    Publish: 2014/10/21

  • RSS
  • YouTube
  • Linkedin
  • Facebook