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AbVideo™ - Western Blot

   
  • Western Blot

    Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein.

    Publish: 2010/1/10

  • Western Blot Washer

    There are several wash steps involved in western blot protocol. Abnova designed a western blot washer machine which can change the washing buffer automatically and shake by programs we setup.

    Publish: 2010/5/24

  • Western Blot Membrane Stripping

    Stripping is used to remove primary and secondary antibodies from a western blot membrane to allow the incubation of new antibodies. It is useful to save samples, materials, and time when you want to investigate more than one protein on the same blot.

    Publish: 2010/12/17

  • Western Blot - Ponceau S Staining

    Ponceau S is a rapid and reversible staining method for locating protein bands on Western blots producing reddish pink stained bands. The stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and can be completely removed from the protein bands by continued washing.

    Publish: 2010/11/26

  • Microwestern Arrays - Lecture Series 3

    Lecture series 3 demonstrates Dr. Chuu’s microwestern array experiments in details and also explains his results using different analyses. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 4

    Lecture series 4 points out the future direction and potential application using microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 1

    Lecture series 1 compares different array technologies and reveals their limitations and current challenges which brings out the importance of microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 2

    Lecture series 2 explicitly explains the step-by-step guide of performing microwestern arrays. Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Western Blot Substrate Kit - Introduction

    ( http://www.abnova.com ) - WB substrate kit detection reagents are non-isotopic, luminol-based chemiluminescence substrate, designed for the chemiluminescent detection of immobilized proteins and immobilized nucleic acids conjugated with horseradish peroxidase (HRP). The peroxidase-catalyzed oxidation of luminol produces a weak flash of light at 425 nm. The incorporation of an electron transfer mediator into the buffer forces the flash signal into a glow and greatly improves the analytical characteristics of the reaction in terms of increased signal intensity and duration. Recent works have shown that, by addition of a suitable acylation catalyst, a further large increase in light output is observed. Here is a brief introduction to why you should choose our products for your fluorescence blotting. More videos at Abnova http://www.abnova.com

    Publish: 2014/8/26

  • Western Blot - SNAP id™ System

    SNAP i.d.™ System is a fast and convenient method for the detection of immunoreactive proteins on western blots. The amount of time required for immunodetection is greatly reduced in 30 minutes with this unique vacuum-driven system.

    Publish: 2010/9/27

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