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AbVideo™ - Tissue Culture

   
  • Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)

    Human umbilical vein endothelial cells (HUVEC) are commonly used as a laboratory model system for the physiological and pharmacological investigations. This video describes how to derive HUVEC from the endothelium of veins of the umbilical cord.

    Publish: 2013/3/29

  • Mycoplasma Detection - Direct Method

    “Direct method” for detecting Mycoplasma in the cell samples is provided. Using microtest to determine if mycoplasmal contaminant is present stands one of the most significant cell culture procedures.

    Publish: 2011/8/8

  • hESCs Cryopreservation

    Human embryonic stem cells (hESCs) are pluripotent cells able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. This video describes how to prepare cryopreserved hESCs for long-term storage.

    Publish: 2012/6/7

  • Mycoplasma Assay

    Mycoplasma is highly infectious and cross-contamination commonly occurs when new cells are introduced into laboratories. We use PCR-based method for detection of Mycoplasma weekly. It is a highly sensitive, specific and rapid method to detect mycoplasma contamination in cell cultures.

    Publish: 2010/3/22

  • Subculture of hESCs with Collagenase IV

    This video shows how to subculture hESCs by collagenase IV. hESCs subculture is used to maintain master stocks of undifferentiated hESCs and minimize cell damage.

    Publish: 2012/6/7

  • Mycoplasma Detection - Hoechst DNA Staining

    This AbVideo tells you how to detect Mycoplasma in vero cell samples through the “Hoechst DNA staining method”, since one of the most important cell culture procedures is to use microtest to identify whether mycoplasmal contaminant is present or not.

    Publish: 2011/8/8

  • Coated Plate Preparation

    Plate surface that is coated with poly-D-lysine possesses a uniform net positive charge. This uniform net positive charge is preferred by certain cell types and can subsequently enhance cell attachment, growth and differentiation.

    Publish: 2011/4/19

  • Electroporation

    Electroporation applies a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules to pass into the cell. It is usually used in molecular biology as a way of introducing some substance into a cell, such as molecular probes, drugs or pieces of coding DNA.

    Publish: 2010/11/22

  • Aliquoting and Preparation of Serum before Usage

    This is recommended to creat aliquots of serum when only small volumes are used at a time. This video shows you how to thaw the serum, aliquot into the 50ml tubes and refreeze.

    Publish: 2011/5/31

  • Integra CL350 Cell Cultivation

    The CELLine bioreactor is a disposable, two-compartment cultivation device suitable for many cell culture applications. There are two sizes of the CELLine, CL350 and CL 1000. We use CL350 to produce monoclonal antibodies on a laboratory scale.

    Publish: 2010/6/30

  • Cell Counting (Attached Cells)

    This episode of the cell counting series demonstrates the handling of attached cell before counting. Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.

    Publish: 2010/3/1

  • Bacteria/Fungus Detection

    The detection of bacteria and fungus is used as part of a routine and regular quality control screening procedure in cell culture. These contaminations are generally visible to the naked eye and detected by a sudden increase in turbidity and color change of the culture medium as the result of a change in pH.

    Publish: 2010/3/22

  • Cell Freezing Equipment - Styrofoam Box

    The Styrofoam box slows the rate of freezing, so that ice crystals won’t form too fast and destroy the cells. This AbVideo provides instructions on how to use Styrofoam box for cell freezing and shows the procedure for you.

    Publish: 2011/5/31

  • Cell Freezing Equipment - CoolCell®

    The "CoolCell" is an alcohol-free freezing container which provides the ideal freezing rate close to minus 1°C/minute required for successful cryopreservation of cells. This video demonstrates you the steps of how to use it.

    Publish: 2011/5/31

  • Gelatin Preparation and Coating

    Gelatin solution is used to coat 6-well plates for culture of Mouse Embryonic Fibroblasts (MEFs). MEFs may be used as feeder cell layer to support the survival and growth of human embryonic stem cells (hESCs). This video shows the steps for preparation of gelatin solution and gelatin-coated plates.

    Publish: 2011/8/2

  • Freezing of Adherent Cells

    This AbVideo illustrates the process of adherent cells freezing. Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. The cryopreservation agent and condition can depend on cell type.

    Publish: 2011/3/11

  • Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)

    Mycoplasma is a common and serious contamination of cell cultures. Mycoplasma contamination can cause the alteration of the phenotypic cell characteristics. PCR method allows for fast and reliable identification of mycoplasma contamination in cell cultures.

    Publish: 2013/4/8

  • BD Matrigel™ Coating

    BD Matrigel Coating plays a basic but crucial role for BD Matrigel matrix, and this video presents you the steps of its procedure. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media.

    Publish: 2011/12/2

  • Cell Culture Medium Preparation

    This video demonstrates the steps to prepare cell culture medium from media powder.

    Publish: 2011/4/19

  • Stem Cell Embryoid Body Formation

    This video shows a novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel. [Reference: Biomacromolecules. 2007 Sep;8(9):2746-52]

    Publish: 2010/10/21

  • Mycoplasma Detection Agar Preparation

    Mycoplasma detection agar (without crystal violet) is used with enrichments for the identification and cultivation of Mycoplasma. This video demonstrates the steps to prepare Mycoplasma agar.

    Publish: 2011/12/2

  • BD Matrigel™ Aliquoting

    This AbVideo shows you how to create aliquots of BD Matrigel when only small volumes are used at a time. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media.

    Publish: 2011/12/2

  • Cationic Polymer Transfection

    jetPEI™, a polyethylenimine (PEI) derivative, is a cationic polymer transfection reagent that binds to DNA by electrostatic forces. The cationic polymer/DNA complex enters the cell by endocytosis, where the DNA is then released into the cytoplasm.

    Publish: 2011/2/11

  • Handling Adherent Cells upon Receipt

    Handling of adhering cell is the key point before culturing. This video shows you how to disinfect the flask well, confirm the condition of the cells under microscopy, and re-attach the cell that may have detached during transportation.

    Publish: 2011/5/27

  • Cell Culture (Suspension Cell)

    Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture suspension cells from cell thawing, cell maintenance, to cell freezing.

    Publish: 2017/5/26

  • Handling Suspension Cells upon Receipt

    Handling of suspending cell is the key point before culturing. This video shows you how to disinfect the tube well, transfer the cell suspension to a flask or dish, and make sure the condition of the cells under microscopy.

    Publish: 2011/5/27

  • Calcium Phosphate Transfection

    Calcium phosphate transfection method is a simple, time efficient and relatively inexpensive way to introduce DNA into cells in stable cell lines such as HEK293T, CHO and BHK cells. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.

    Publish: 2010/7/5

  • Subculture of Suspension Cells

    This AbVideo demonstrates how to subculture the suspension cells since passaging the cells is a crucial step for growing cell cultures to avoid overcrowding. Another good reason for subculturing is to gain more cell samples for research use.

    Publish: 2011/2/25

  • RNAi Transfection

    RNA interference is a gene-silencing technique used in studying the absence of normal gene action. To better understand its function and role in disease, you can transfect siRNA into cell line to turn gene expression off or knock it down.

    Publish: 2010/2/12

  • Chicken Myocyte Primary Culture

    Primary culture of chick embryo myocyte culture can be used for the detection and assessment of drug responses on cardiomyocyte.

    Publish: 2014/3/25

  • Cell Thawing

    The thawing procedures for regular (non-DMSO-sensitive) cells are performed. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells.

    Publish: 2011/3/11

  • Cell Freezing Equipment - Mr. Frosty™

    This AbVideo shows the procedure for using "Mr. Frosty". "Mr. Frosty" is a freezing container filled with isopropyl alcohol at room temperature. It offers the ideal cooling rate close to minus 1°C/minute required for effective cryopreservation of cells.

    Publish: 2011/5/27

  • Embryoid Body Formation - Ultra-Low Attachment Dishes

    Embryoid Bodies (EB) Formation is an in vitro method to evaluate the pluripotency of the cells. EBs are formed in ultra low attach dishes that promote the aggregation of cells.

    Publish: 2012/6/7

  • Cell Freezing Equipment - Mechanical Freezer

    How to transport cryovials from 4°C, -20°C, -80°C mechanical freezers to liquid nitrogen tank while maintaining optimal cell health is presented in this video. Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. The cryopreservation agent and condition can depend on cell type.

    Publish: 2011/5/27

  • Insect Cell Culture

    The Sf9 cell line derived from pupal ovarian tissue of the Fall armyworm Spodoptera frugiperda is one of the most common cell lines used for BEVS (Baculovirus expression vector systems). This video shows you how to culture the Sf9 cell line.

    Publish: 2010/5/31

  • Cell Freezing Equipment - Programmable Freezer

    This video displays the procedure for cell freezing with programmable freezer. By using programmable freezer, you could control and ensure the freezing rates are exactly as required. It is the most reliable and reproducible way to freeze cells.

    Publish: 2011/5/27

  • Cell Culture (Attached Cells)

    Cells are cultured under précised conditions; A complete protocol on how to thaw, maintain and freeze attached cells is shown in this AbVideo.

    Publish: 2017/4/28

  • Subculture of hESCs with Mechanical Dissection

    This video demonstrates how to subculture hESCs by mechanical dissection. Typically, hESCs subculture is used to maintain master stocks of undifferentiated hESCs and minimize cell damage.

    Publish: 2012/5/3

  • Cell Thawing for DMSO-Sensitive Cells

    This AbVideo describes how to thaw DMSO-sensitive cells. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells.

    Publish: 2011/3/11

  • Bacterial/Fungal Contaminant Detection

    It s very important to test cell lines and cell culture media to determine if microbial contamination is present. The culture media used for the tests are tryptic soy broth (TSB) for the detection of aerobic and anaerobic bacteria, and fluid thioglycollate medium (FTM) for the detection of erobes, facultative anaerobes and fungi.

    Publish: 2011/8/8

  • Thawing Inactivated Feeder Cells (MEFs)

    Mouse embryonic fibroblasts (MEFs) have been used as feeder cell layers for the culture of Human Embryonic Stem (hES) cells. MEFs could promote optimum growth and prevent differentiation of hESCs. This video describes how to thaw MEF cells and seed in plate for usage.

    Publish: 2011/12/2

  • Mycoplasma Detection Broth Preparation

    Mycoplasma detection broth (without crystal violet) is used for the isolation and cultivation of Mycoplasma for research studies. This video shows you how to prepare the Mycoplasma broth.

    Publish: 2011/12/2

  • Subculture of Adherent Cells

    Growing cell cultures requires subculturing (or passaging) every few days to avoid overcrowding and to increase the number of cell samples for research use. This video teaches you how to subculture adherent cells.

    Publish: 2011/2/25

  • Cell Counting (Cell Suspension)

    This AbVideo introduces the protocol of preparing suspension cell for counting. Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.

    Publish: 2010/3/1

  • Cell Counting

    Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.

    Publish: 2011/3/11

  • hESCs Thawing

    This video describes how a vial of cryopreserved hESCs is thawed and seeded on a feeder layer of inactivated Mouse Embryonic Fibroblasts (MEFs). Human embryonic stem cells (hESCs) are pluripotent cells able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm.

    Publish: 2011/12/2

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