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AbVideo™ - RNA

   
  • Reverse Transcription PCR

    Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR.

    Publish: 2017/10/2

  • RNA Extraction from Tissue

    Difficulties of isolating intact total RNA from tissue samples vary with the physical and biochemical nature of tissue. This AbVideo demonstrates how we can extract total RNA from tissue. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2015/10/19

  • mRNA Extraction

    Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

    Publish: 2010/4/19

  • RNA Gel Preparation

    The quality of RNA preparation could be measured by electrophoresis on a denaturing agarose gel. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the formaldehyde agarose gel for the electrophoresis of RNA.

    Publish: 2011/1/6

  • DEPC Treatment

    Diethylpyrocarbonate (DEPC) is used in the laboratory to inactivate the RNase enzymes from water and other laboratory utensils. It inactivates the RNases by the covalent modifications of the histidine residues. DEPC treated (and therefore RNase-free) water is used in handling of RNA to reduce the risk of RNA being degraded by RNases.

    Publish: 2011/4/19

  • RNA Extraction by TRIzol®

    TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

  • RNA Electrophoresis

    RNA electrophoresis is an analytical technique used to separate RNA fragments. RNA has the tendency to form both secondary and tertiary structures that can impede its separation. So a denaturing condition is performed in formaldehyde and MOPS buffer system. RNA under this condition is fully denatured and migrates according to its molecular weight.

    Publish: 2011/2/16

  • Northern Blot

    The northern blot is used to study gene expression by detection of RNA (or isolated mRNA). A general blotting procedure starts with the separation of RNA by electrophoresis. The RNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.

    Publish: 2011/1/21

  • RNA Extraction from Cell Culture

    Extracting RNA from cultured cells has traditionally been a highly repetitive method, and this AbVideo indicates the steps on how to do it. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.

    Publish: 2016/3/28

  • Chimera RNA Interference

    Chimera RNAi is a process by which small interfering RNA/DNA chimera triggers the destruction of mRNA. The discovery work, design, and application of chimera RNAi have been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.

    Publish: 2016/12/30

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