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AbVideo™ - Microbial Culture

   

  • TSB Medium Preparation

    Tryptic Soy Broth (TSB) is a general purpose culture broth used to support the growth of a wide variety of aerobic, facultative and anaerobic bacteria. This video demonstrates the steps to prepare TSB medium.

    Publish: 2011/8/2

  • Fluid Thioglycollate Medium Preparation

    Fluid Thioglycollate Medium (FTM) is a multi-purpose enriched differentiating media designed to test the aerotolerance of a wide range of bacteria. This video presents the process for preparation of FTM.

    Publish: 2011/8/2

  • Lentivirus RIU Test

    This video presents the protocol for relative titer method for lentivirus. Lentivirus titers are estimated as relative infectious units (RIU), according to the curve of cell viability versus viral dose.

    Publish: 2011/8/2

  • LB Medium Preparation

    LB broth is used for maintaining and cultivating recombinant strains of Escherichia coli. The ingredients of LB broth are tryptone, yeast extract and Sodium Chloride. We show you how to prepare the LB medium.

    Publish: 2010/7/22

  • Plaque Assay for Influenza Virus

    The plaque assay is a widely used approach for purifying a clonal population of virus and determining the viral titers (the lowest concentration of virus that results in infection). This video shows the procedure of plaque assay for influenza virus.

    Publish: 2013/4/8

  • Adenovirus Infection

    This video shows you how to infect culture cells with adenoviruses. It is an important step before the production of adenoviral vector. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.

    Publish: 2011/5/31

  • Particle Blot for HBV Particle

    Hepatitis B viruses (HBV) are double-stranded DNA viruses that cause acute and chronic hepatitis. Furthermore, HBV infection is the leading cause of liver cancer. This video shows the procedure for using particle blot to analyze HBV Particle.

    Publish: 2011/6/17

  • Adenoviral Vector Production

    This video demonstrates how to produce adenoviral vector after adenoviruses infection. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.

    Publish: 2011/6/17

  • Terrific Broth (TB) Preparation

    Terrific Broth (TB) is an enriched medium used with glycerol in cultivating Escherichia coli. TB allows the bacteria to produce a greater number of recombinant proteins and plasmid yield. This video shows you how to prepare TB.

    Publish: 2011/7/1

  • Covalently Closed Circular DNA (cccDNA) Isolation

    cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.

    Publish: 2011/6/29

  • Lentiviral Production

    Lentiviruses are able to deliver genes into almost any mammalian cell type and have the ability to replicate in non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. This video presents the process of lentiviral production in 6cm plate.

    Publish: 2011/3/21

  • Lentiviral Infection

    Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector.

    Publish: 2011/7/1

  • Competent Cell Preparation

    Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. Because DNA is hydrophilic, it will not normally pass through membrane. In order to make these cells readily incorporate foreign DNA, they must first be made "competent" to take up foreign DNA.

    Publish: 2010/7/5

  • LB Plate Preparation

    An agar plate is a Petri dish that contains a growth medium (typically agar plus nutrients) used to culture microorganisms. We show you how to prepare the LB agar plate.

    Publish: 2010/7/22

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