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AbVideo™ - Methods

   
  • Dot Blot

    Dot blot is a technique can be used as a semiqualitative method for rapid screening of a large number of samples. It is for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane.

    Publish: 2017/2/16

  • Mononuclear Cell Isolation from Whole Blood

    This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps.

    Publish: 2010/5/31

  • Volume Estimation in Pipette Tips

    It is important to get a feel for the appearance of volume levels of pipette tips. We show you how to recognize volumes in pipette tips .It may help you catch a mistake before it happens.

    Publish: 2010/8/5

  • Mouse Genotyping

    Genotyping is the process of determining the genes (genotype) of an individual by examining its DNA with the use of biological assays (ex: PCR, DNA sequencing). Three steps to type the mouse genotype : (i) isolate the genomic DNA from mouse tails; (ii) PCR to amplify with specific primers; (iii) electrophoresis to determine the genotype.

    Publish: 2010/8/26

  • Optical Fiber Cleavage

    This AbVideo demonstrates the operation of automated fiber cleavage to produce flat and angled cleaves on fibers ranging from 80 microns to 1.25 mm in diameter for following biological applications. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/3

  • Cell Synchronization with Nocodazole at Mitosis

    Cell Synchronization is a process by which cells at different stages of the cell cycle in a culture are brought to the same phase. Nocodazole is one of anticancer drugs that synchronize cells at M-phase. It can interfere with the structure and function of microtubules in interphase and mitotic cells.

    Publish: 2010/12/17

  • Blot Imaging with X-Ray Film

    An X-ray film can detect both the radioactive and chemiluminescent signals from Western, Northern or Southern blot. It is placed against the blot membrane in a light-proof cassette. After the exposure for an appropriate time period, the film is developed by an auto-processor in a darkroom.

    Publish: 2011/2/16

  • Cell Fixation

    The cells must be fixed and permeabilized to ensure free access of the antibody to its antigen. Fixation methods fall generally into two classes: organic solvents and cross-linking reagents. This video uses formaldehyde as a cross-linking reagent to fix cells.

    Publish: 2010/8/30

  • Syringe Filter

    A syringe filter is a single-use filter cartridge. It can be used to remove particles from a sample. We’ll show you how to use a syringe filter.

    Publish: 2011/4/19

  • FastLink R-PE Labeling Kit

    The FastLink R-PE Labeling Kit allows PE conjugations to set up in seconds, simply by adding a solution of the antibody to be labeled to a proprietary lyophilised mixture containing PE. By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, FastLink technology can be used to label small quantities of protein for FACS analysis with 100% recovery. This video shows the procedure for setting up conjugation reactions.

    Publish: 2011/6/29

  • FastLink HRP Labeling Kit

    The FastLink HRP Labeling Kit allows conjugations to set up in seconds, simply by adding a solution of the protein to be labeled to a proprietary lyophilised HRP mixture. By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, FastLink technology can be used to label small quantities of protein with 100% recovery and no excessive dilution of the conjugate. This video shows you how to set up conjugation reactions.

    Publish: 2011/6/29

  • Dialysis

    Dialysis (ultrafiltration) is a method to concentrate protein or other macromolecules through a membrane with defined pores. The membranes will have a molecular weight cut-off (MWCO). This is the limit of size (or range) of a protein that can fit through the membrane.

    Publish: 2010/5/17

  • Making a Glass Knife

    This video shows you how to make a glass knife with capillary or pasteur pipette.

    Publish: 2011/8/2

  • Optical Fiber End-Face Analysis

    This AbVideo demonstrates the operation of automated interferometric inspection system specifically designed for checking the surface quality and flatness of cleaved, polished or lensed fibers. Optical fibers are flexible, transparent fibers made of high quality extruded glass (silica) or plastic. It is frequently adopted as a waveguide, or "light pipe", to transmit the optical signals. Taking advantages of this kind of property, optical fibers have been used in developing biological sensors in concert with traditional bio-probes such as nucleic acids.

    Publish: 2014/6/10

  • Lyophilization

    Biological materials often are dried to stabilize them for storage or distribution. Lyophilization also called freeze-drying, is one method of drying biological materials that minimizes damage to its internal structure.

    Publish: 2010/6/30

  • Serial Dilution

    A serial dilution is a series of simple dilutions which amplifies the dilution factor quickly beginning with a small initial quantity of material. The dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.

    Publish: 2010/8/19

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