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AbVideo™ - Lysates

   
  • Transfected Lysate Preparation (Denatured)

    This AbVideo demonstrates how to prepare as ready-to-use denatured lysate in 1x sample buffer. Transfected lysate is useful as a positive control in western blot and the full-length protein construct is expressed in HEK293T cells.

    Publish: 2010/1/29

  • Transfected Lysate Preparation (Native)

    This AbVideo shows the steps involved on preparation of ready-to-use, native lysate in modified RIPA buffer. Transfected lysate is useful as a positive control in western blot and the full-length protein construct is expressed in HEK293T cells.

    Publish: 2010/1/29

  • Tissue Lysate Preparation (Native)

    Tissue lysate preparation is explained in this protocol which exemplifies the procedure with three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.

    Publish: 2010/6/7

  • Nuclear and Cytoplasmic Protein Extraction

    Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells.

    Publish: 2011/4/19

  • Cell Nuclear Protein Extraction

    Nuclear protein fractionation is proposed as a method to be carried out according to the solubility of proteins in buffers of increasing ionic strength. This protocol indicates the procedure for the preparation of nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer.

    Publish: 2010/5/31

  • Cell Lysate Preparation (Denatured)

    Denaturation is a process in which proteins or nucleic acids lose their native structure due to external stress or compound. This AbVideo shows the protocol involves three steps for denatured lysate preparation. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, denatured lysate in 1x Sample Buffer.

    Publish: 2015/12/31

  • Cell Lysate Preparation (Native)

    Here we display you the protocol on how to prepare cell lysate. This includes 3 steps: First, cells are harvested. Then, cells are lysed in lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.

    Publish: 2015/9/24

  • Tissue Lysate Preparation (Denatured)

    This video demonstrates tissue lysate preparation when denatured and involves three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, denatured lysate in 1x Sample Buffer.

    Publish: 2010/6/7

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