AbVideo™ Browser

AbLearning™
- CAR T Cell Therapy: Efficacy & Safety
- Chimeric Antigen Receptor T Cell Therapy
- Circulating Tumor Cell Isolation
- Circulating Tumor Cells
- T Cell Immunometabolism
Antibody
- Antibody Conjugation - Biotinylation
- Antibody Conjugation - FITC
- Antibody Pair for Proximity Ligation Assay
- Antibody Purification (Affinity)
- Antibody Purification (Protein A Column)
- Chicken Antibody Extraction
- Chicken Antibody Introduction
- Chicken Immune System
- Chicken Immunization
- Emulsification (CFA and IFA)
- The Advantages of Chicken Antibody
Beads
- MaxBead Carboxyl Labeling Kit Operation
- MaxBead Protein A/G Operation
Bioinformatics
- BLAST - Multiple Alignment
- BLAST - Nucleotide
- BLAST - PCR Primer Design
- BLAST - Protein
- Cytoscape
- EHCO - Encyclopedia of Hepatocellular Carcinoma Genes Online
- FASTA Formatted Sequence Record
- Gene Conserved Domains
- Gene Homology
- Gene Intron-Exon
- Gene Map Viewer
- Gene ORF Finder
- Gene Pathway
- Gene SNP
- GeneCards®
- GEO DataSets
- Human Protein Reference Database
- OMIM Database
- POINeT - Protein Interactome Datasets
- Protein Structure
- PubMed Literature Database
Calibration
- Autoclave Quality Control Test - Biological Indicator
- Autoclave Quality Control Test - Temperature Logger
- Micropipette Calibration
- pH-meter Calibration
Cell Assays
- alamarBlueR® Cell Proliferation Indicator
- Antibody Array for Protein Expression Profiling
- ApoTox-Glo™ Triplex Assay
- Cell Proliferation Assay (MTS)
- Cell Proliferation Assay (WST-1)
- Clonogenic Assay
- Invasion Assay
- Lactate Dehydrogenase Assay Kit
- Luciferase Assay
- Migration Assay
- MTT Assay
- MTT Assay (by BCRC)
- Raw Cell Transmigration
Cell Biology
- BAD in Cancer Signalling
- The Ciliopathy-associated Protein Homologs RPGRIP1
Cell Staining
- Alizarin Red S Staining for Osteogenesis
- Alkaline Phosphatase Staining
- Oil Red O Staining for Adipogenesis
- Propidium Iodide Staining
- Safranin Staining for Chondrogenesis
- Wright and Giemsa Staining
Clinical
- Blood Smear
- Down Syndrome
- Edwards Syndrome
- Hematology Test
- Klinefelter Syndrome
- Patau Syndrome
- Prenatal Tests for Genetic Disorders
- Trisomy X Syndrome
- Urinalysis
- Warsaw Breakage Syndrome
- Williams-Beuren Syndrome
- Wolf-Hirschhorn Syndrome
CytoQuest™ CR
- CytoQuest™ CR Automated Leucosep Preparation
- CytoQuest™ CR Bubbles & Microfluidics - Dos and Don`ts
- CytoQuest™ CR Capture Antibody Immobilization
- CytoQuest™ CR Cell Capture
- CytoQuest™ CR Cell Immunostaining
- CytoQuest™ CR CTC Capture and CTC FISH - Dos and Don`ts
- CytoQuest™ CR CTC FISH
- CytoQuest™ CR Installation
- CytoQuest™ CR Leucosep Preparation
- CytoQuest™ CR Plugs Assembly and CytoChipNano Placement - Dos and Don`ts
- CytoQuest™ CR SCx™ Spiral Chamber Assembly - Dos and Don`ts
- CytoQuest™ CR System Maintenance
- CytoQuest™ CR Unpacking
CytoView™
- CytoView™ All-in-One Imaging System
DNA
- Agarose DNA Gel Preparation (by BCRC)
- Cre-Lox Recombination
- DNA Electrophoresis
- DNA Extraction by Phenol/Chloroform
- DNA Extraction from Mouse Tail (Manual)
- DNA Gel Preparation
- DNA Sequencing
- Gateway® Recombination
- Gel Extraction
- Methylation-specific PCR
- PCR Cloning
- PCR Cloning (I) - Inserted Fragment Preparation
- PCR Cloning (II) - Digestion
- PCR Cloning (III) - Ligation
- PCR Cloning (IV) - Transformation
- PCR Cloning (V) - Colony Selection
- PCR Mutagenesis
- PCR Purification
- Plasmid DNA Extraction (CsCl Isolation)
- Plasmid DNA Extraction (Gigaprep)
- Plasmid DNA Extraction (Megaprep)
- Plasmid DNA Extraction (Midiprep)
- Plasmid DNA Extraction (Miniprep)
- Plasmid DNA Extraction (Traditional Alkaline Lysis Method)
- Polymerase Chain Reaction
- Real-Time PCR
- Southern Blot
Dye
- Fluorescent Dye - Differential Gel Electrophoresis
- Fluorescent Dye - Introduction
- Fluorescent Dye - Protein Labeling
ELISA
- Anti-PEG Antibody Pair
- Competitive ELISA
- ELISA Substrate Kit - Introduction
- FSH ELISA Kit
- HCG ELISA Kit
- Indirect ELISA
- LEP (Human) Clinical Range ELISA Kit
- Pork (Pig) ELISA Kit
- Sandwich ELISA
- Sandwich ELISA - Isotype Detection
- Total IgG (Human) ELISA Kit
Equipment
- Micropipette Guideline
- Pipette (Glass) Washing
- Sonicator
- Tissue Grinder
FISH
- Fluorescence In Situ Hybridization (FISH)
- mutaFISH™ - How It Works
- mutaFISH™ (Circulating Tumor Cell)
- mutaFISH™ (Frozen Tissue)
Flow Cytometry
- Flow Cytometry - Cell Cycle
- Flow Cytometry - Double Staining
- Flow Cytometry - Single Staining
- Start-up and Clean-up Procedures for Flow Cytometer
Immunofluorescence
- Immunofluorescence
Immunohistochemistry
- H&E Staining
- IHC Kit - RealBlue Peroxidase Substrate Kit
- Immunohistochemistry
- Rotary Microtome Section
- Tissue Embedding
- Tissue Microarray
Immunoprecipitation
- Immunoprecipitation
Instruments
- Atomic Force Microscopy(AFM) Basic Tutorial
- BioSpectrum® Imaging System
- Cell Counting - Cellometer®
- CO₂ Analyzer
- CO₂ Indicator
- Fluorescent Microscope
- Micro-Manipulator - Probe Preparation
- Micro-Manipulator - Single Cell Collection
- SpiralPipet™
- UV-Vis Spectrophotometer - Nanodrop®
Isotope
- Isotope Labeling
- Radioactive Signal Detection
Lysates
- Cell Lysate Preparation (Denatured)
- Cell Lysate Preparation (Native)
- Cell Nuclear Protein Extraction
- Nuclear and Cytoplasmic Protein Extraction
- Tissue Lysate Preparation (Denatured)
- Tissue Lysate Preparation (Native)
- Transfected Lysate Preparation (Denatured)
- Transfected Lysate Preparation (Native)
Methods
- Blot Imaging with X-Ray Film
- Cell Fixation
- Cell Synchronization with Nocodazole at Mitosis
- Dialysis
- Dot Blot
- FastLink HRP Labeling Kit
- FastLink R-PE Labeling Kit
- Lyophilization
- Making a Glass Knife
- Mononuclear Cell Isolation from Whole Blood
- Mouse Genotyping
- Optical Fiber Cleavage
- Optical Fiber End-Face Analysis
- Serial Dilution
- Syringe Filter
- Volume Estimation in Pipette Tips
Microbial Culture
- Adenoviral Vector Production
- Adenovirus Infection
- Competent Cell Preparation
- Covalently Closed Circular DNA (cccDNA) Isolation
- Fluid Thioglycollate Medium Preparation
- LB Medium Preparation
- LB Plate Preparation
- Lentiviral Infection
- Lentiviral Production
- Lentivirus RIU Test
- Particle Blot for HBV Particle
- Plaque Assay for Influenza Virus
- Terrific Broth (TB) Preparation
- TSB Medium Preparation
mutaFISH™
- mutaFISH™ - How It Works
- mutaFISH™ (Circulating Tumor Cell)
- mutaFISH™ (Frozen Tissue)
Protein
- 2D Gel Electrophoresis (1) Protein Extraction
- 2D Gel Electrophoresis (10) Visible Staining
- 2D Gel Electrophoresis (11) Mass Sample Preparation
- 2D Gel Electrophoresis (12) Mass Analysis
- 2D Gel Electrophoresis (2) Protein Quantitation
- 2D Gel Electrophoresis (3) Sample Preparation for the First Dimension
- 2D Gel Electrophoresis (4) The First Dimension
- 2D Gel Electrophoresis (5) Gel Casting
- 2D Gel Electrophoresis (6) Reduction and Alkylation
- 2D Gel Electrophoresis (7) The Second Dimension
- 2D Gel Electrophoresis (8) Fluorescent Staining
- 2D Gel Electrophoresis (9) Image Capture - Laser Scanner
- 2D Gel Electrophoresis (9) Image Capture - UV Light Box
- Gelatin Zymography
- GST Enzymatic Digestion
- GST-tagged Protein Purification
- His-tagged Protein Purification
- Protein Quantification (BCA Assay)
- Protein Quantification (Bradford Assay)
- Protein Quantification (CBR)
- Protein Quantification (OD₂₈₀)
- Protein Quantification (Silver Staining)
- SDS-PAGE
- SDS-PAGE Gel Preparation
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 1
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 2
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 3
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 4
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 5
Protemist®
- Protemist® DT - Automated Wheat Germ Protein Synthesizer
- Protemist® DT II and XE - Demonstration
- Protemist® DT II and XE - Lecture Series
- Protemist® XE - Large-Scale Protein Expression
- Protemist® DT II - High Throughput Protein Expression
- Protemist® DT II - Operation
RNA
- Chimera RNA Interference
- DEPC Treatment
- mRNA Extraction
- Northern Blot
- Reverse Transcription PCR
- RNA Electrophoresis
- RNA Extraction by TRIzol®
- RNA Extraction from Cell Culture
- RNA Extraction from Tissue
- RNA Gel Preparation
Safety
- Bioburden Test of BSC
- Electrical Safety Test
- EtBr Handling
Tissue Culture
- Aliquoting and Preparation of Serum before Usage
- Bacteria/Fungus Detection
- Bacterial/Fungal Contaminant Detection
- BD Matrigel™ Aliquoting
- BD Matrigel™ Coating
- Calcium Phosphate Transfection
- Cationic Polymer Transfection
- Cell Counting
- Cell Counting (Attached Cells)
- Cell Counting (Cell Suspension)
- Cell Culture (Attached Cells)
- Cell Culture (Suspension Cell)
- Cell Culture Medium Preparation
- Cell Freezing Equipment - CoolCell®
- Cell Freezing Equipment - Mechanical Freezer
- Cell Freezing Equipment - Mr. Frosty™
- Cell Freezing Equipment - Programmable Freezer
- Cell Freezing Equipment - Styrofoam Box
- Cell Thawing
- Cell Thawing for DMSO-Sensitive Cells
- Chicken Myocyte Primary Culture
- Coated Plate Preparation
- Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)
- Electroporation
- Embryoid Body Formation - Ultra-Low Attachment Dishes
- Freezing of Adherent Cells
- Gelatin Preparation and Coating
- Handling Adherent Cells upon Receipt
- Handling Suspension Cells upon Receipt
- hESCs Cryopreservation
- hESCs Thawing
- Insect Cell Culture
- Integra CL350 Cell Cultivation
- Mycoplasma Assay
- Mycoplasma Detection - Direct Method
- Mycoplasma Detection - Hoechst DNA Staining
- Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)
- Mycoplasma Detection Agar Preparation
- Mycoplasma Detection Broth Preparation
- RNAi Transfection
- Stem Cell Embryoid Body Formation
- Subculture of Adherent Cells
- Subculture of hESCs with Collagenase IV
- Subculture of hESCs with Mechanical Dissection
- Subculture of Suspension Cells
- Thawing Inactivated Feeder Cells (MEFs)
Website
- Website Tutorial - Product Comparison
Western Blot
- Microwestern Arrays - Lecture Series 1
- Microwestern Arrays - Lecture Series 2
- Microwestern Arrays - Lecture Series 3
- Microwestern Arrays - Lecture Series 4
- Western Blot
- Western Blot - Ponceau S Staining
- Western Blot - SNAP id™ System
- Western Blot Membrane Stripping
- Western Blot Substrate Kit - Introduction
- Western Blot Washer

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ISO

AbVideo™ - Isotope

Isotope Labeling

Isotopic labeling is a technique for labeling a substance with a stable or radioactive isotope. By measuring the radioactivity or the abundance of the isotope, we could made observations of the course through a biologic process. This video shows the procedure for using radioisotope phosphorus-32 to label DNA.

Publish: 2011/7/1
Radioactive Signal Detection

FUJIFILM FLA-2000 is a multiple imaging application suitable for fluorescence and radioisotopic measurement. Laser scanning with the FLA-2000, instead of using conventional photography, permits analysis and digital storage. With FUJIFILM Imaging Plate (IP), a technology which is 100 times more sensitive to radiation than X-ray film, exposure times are reduced to 1/10 - 1/20 of film and amount of radioisotope is reduced.

Publish: 2011/3/21