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AbVideo™ - DNA

   
  • DNA Sequencing

    DNA sequencing is a technique to determine the order of the nucleotide bases-adenine, guanine, cytosine, and thymine. It is indispensable for basic biological research and discovery.

    Publish: 2010/1/14

  • PCR Purification

    Following PCR, you need to get rid of excess short primers, dNTPs, enzymes, short-failed PCR products and salts. We use a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O to get clean PCR products for downstream applications.

    Publish: 2010/3/22

  • PCR Cloning (II) - Digestion

    Digestion is the process of cutting DNA molecules with restriction endonucleases. These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA. In PCR cloning, insert and vector are cutted by the same restriction endonucleases and then join together in the next step, ligation.

    Publish: 2010/3/29

  • PCR Cloning (IV) - Transformation

    The purpose of transformation is to introduce a foreign plasmid into bacteria and the bacteria will replicate the foreign plasmid along with their own DNA. Bacteria which are able to uptake DNA after a heat shock are called "competent".

    Publish: 2010/3/29

  • PCR Cloning (V) - Colony Selection

    It can be used after a transformation to screen colonies for the plasmid. Primers designed for the insert sequence should be used when preparing the PCR reaction. Thus, any colonies which give rise to an amplification product are likely to contain the correct DNA sequence.

    Publish: 2010/3/29

  • PCR Cloning

    PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid.

    Publish: 2010/3/31

  • Cre-Lox Recombination

    Cre-Lox recombination is a special type of site-specific recombination. The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. It is often used in the generation of knockout and conditional knockout animals.

    Publish: 2010/4/12

  • Polymerase Chain Reaction

    Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles.

    Publish: 2017/7/3

  • Real-Time PCR

    Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample.

    Publish: 2010/4/19

  • Plasmid DNA Extraction (Miniprep)

    Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and midiprep

    Publish: 2010/4/26

  • Gel Extraction

    Gel extraction is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. Three basic steps are involved: slicing the bands of interest on UV light exposure, isolating the DNA from those bands, and removing the accompanying salts and stain.

    Publish: 2010/5/24

  • Plasmid DNA Extraction (Midiprep)

    Midiprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 15-25 ml of LB broth and the expected DNA yield is 100-350 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and miniprep.

    Publish: 2010/5/17

  • Methylation-specific PCR

    Methylation-specific PCR (MSP) is a rapid and inexpensive method that can be used to determine the methylation status of DNA. It is a bisulfite conversion based PCR technique. In MSP, two primer pairs are designed for methylated and unmethylated DNA.

    Publish: 2010/8/5

  • Plasmid DNA Extraction (Megaprep)

    Megaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 500 ml~2.5 L of LB broth and the expected DNA yield is 1.5~2.5 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, midiprep, and miniprep.

    Publish: 2010/5/24

  • DNA Electrophoresis

    DNA electrophoresis is an analytical technique used to separate DNA fragments based on size, electrical charge and other physical properties. The DNA separates out into bands, with the distance from the electrode corresponding to length of the strand. The technique plays a role in identifying genes for diagnosing disease and for other forms of genetic research.

    Publish: 2010/10/25

  • Agarose DNA Gel Preparation (by BCRC)

    This AbVideo is contributed by Bioresource Collection and Research Center and provides thorough instructions on preparing the agarose DNA gel. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.

    Publish: 2012/5/3

  • Southern Blot

    The Southern blot is used to detect a specific DNA sequence in DNA samples. A general blotting procedure starts with the separation of digested DNA fragments. The DNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.

    Publish: 2011/2/16

  • DNA Extraction by Phenol/Chloroform

    This method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing phenol and chloroform, resulting in an upper aqueous phase and a lower organic phase (mainly chloroform). DNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

    Publish: 2011/1/6

  • Plasmid DNA Extraction (CsCl Isolation)

    CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method.

    Publish: 2011/8/30

  • DNA Extraction from Mouse Tail (Manual)

    DNA extraction is a routine procedure to collect DNA for subsequent molecular analysis. This video shows you how to extract DNA from mouse tails. There are three basic steps: (i) break cells by lysis buffer, (ii) digest protein and remove contamination by proteinase, (iii) precipitating the DNA.

    Publish: 2010/8/30

  • DNA Gel Preparation

    This AbVideo demonstrates step by step of the agarose gel preparation for DNA electrophoresis. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.

    Publish: 2010/10/4

  • Plasmid DNA Extraction (Gigaprep)

    Gigaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 2.5~5 L of LB broth and the expected DNA yield is 7.5~10 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes megaprep, midiprep, and miniprep.

    Publish: 2010/5/24

  • PCR Cloning (I) - Inserted Fragment Preparation

    The procedure of PCR cloning includes DNA preparation, digestion, ligation and transformation. This video shows the first part- insert PCR. Compared to other Taq-generated PCR inserts, Pfu DNA polymerase-generated PCR fragments will have fewer errors and is more commonly used for molecular cloning.

    Publish: 2010/3/22

  • PCR Cloning (III) - Ligation

    DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3 hydroxyl ends of one nucleotide with the 5 phosphate end of another. In PCR cloning, insert DNA and vector digested by the same restriction enzyme can be ligated by DNA ligase.

    Publish: 2010/3/29

  • Gateway® Recombination

    The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. In contrast, Gateway recombination cloning uses a one hour, 99%-efficient, reversible recombination reaction, without using restriction enzymes, ligase, and subcloning step. It is an extremely fast and efficient way to clone your gene.

    Publish: 2010/4/12

  • Plasmid DNA Extraction (Traditional Alkaline Lysis Method)

    Alkaline lysis is one of the most generally useful methods for isolating circular plasmid DNA from bacterial cells. This video demonstrates the procedures for alkaline lysis method.

    Publish: 2011/8/2

  • PCR Mutagenesis

    PCR can be used to make changes to the nucleotide sequence of DNA. This is called PCR mutagenesis. Site-direct mutagenesis is one kind of PCR mutagenesis, which introduces a mutation at a specific location on the DNA strand.

    Publish: 2017/8/1

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