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AbVideo™ - Cell Assays

  • Antibody Array for Protein Expression Profiling

    Abnova offers a variety of arrays for protein expression profiling. These arrays are designed for researchers to study highly relevant proteins in the specific research fields like Angiogenesis, Apoptosis, Cancer Marker, Cell Cycle, Cytokine, Hematopoiesis, Hormone, Signal Transduction and Stem Cell.

    Publish: 2009/11/10

  • ApoTox-Glo™ Triplex Assay

    The ApoTox-Glo™ Triplex Assay combines three Promega assay chemistries to assess viability, cytotoxicity and apoptosis within a single assay well. The first part of the assay simultaneously measures two protease activities: GF-AFC is used to measure cell viability, and bis-AAF-R110 to cytotoxicity. The second part of the assay uses the Caspase-GloR to measure caspase activity.

    Publish: 2010/12/6

  • alamarBlueR® Cell Proliferation Indicator

    alamarBlueR works as a cell viability and proliferation indicator through the conversion of resazurin to resorufin. Resazurin, a non-fluorescent indicator dye, is converted to highly red fluorescent resorufin via reduction reactions of metabolically active cells. The amount of fluorescence produced is proportional to the number of living cells.

    Publish: 2010/12/6

  • Raw Cell Transmigration

    The ability of cell migration is commonly used for the evaluation of cell invasion or wound repair. Using boyden chamber is convenient for the assessment of cells responses toward genetic manipulation or chemo attraction treatment.

    Publish: 2014/3/17

  • Invasion Assay

    Cell invasion assay is designed to accelerate the screening process for compounds that influence cell migration through extracellular matrices. Matrigel (BD), extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, resembles the extracellular matrices is used by cell biologists as a substrate in cell invasion assay.

    Publish: 2010/11/12

  • MTT Assay

    A demonstration on the procedure of using MTT assay to assess the viability and the proliferation of regular cells with absorbance detection at 595nm is shown. This colorimetric assay measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase.

    Publish: 2010/8/26

  • Clonogenic Assay

    Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate.

    Publish: 2017/9/1

  • Cell Proliferation Assay (WST-1)

    The Cell Proliferation Reagent WST-1 is a clear, slightly red, ready-to-use solution, containing WST-1, a tetrazolium salt. By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of 440 nm.

    Publish: 2011/3/21

  • Cell Proliferation Assay (MTS)

    CellTiter 96R AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound (MTS). The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at 490nm.

    Publish: 2011/3/21

  • Luciferase Assay

    Firefly luciferase is widely used as a reporter to study gene expression and other cellular events. The Luciferase Assay is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Through the assay, light is produced by converting the chemical energy of luciferin oxidation and can be measured by a luminometer.

    Publish: 2011/3/21

  • Lactate Dehydrogenase Assay Kit

    Lactate Dehydrogenase Assay Kit is a colorimetric kinetic determination of lactate dehydrogenase activity. Lactate Dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. This video shows the procedures of the kit.

    Publish: 2012/4/5

  • Migration Assay

    The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted.

    Publish: 2010/11/4

  • MTT Assay (by BCRC)

    Bioresource Collection and Research (BCRC) provided this video to introduce the protocol of MTT assay. MTT assay allows assessing the viability and the proliferation of cells. This is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase.

    Publish: 2011/8/2

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