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AbVideo™ - Antibody

   
  • Antibody Pair for Proximity Ligation Assay

    The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation) in tissue and cell samples prepared for microscopy. Each detected signal is visualized as an individual fluorescent dot, these signals can be quantified (counted) and assigned to a specific subcellular location based on microscopy images.

    Publish: 2009/11/10

  • Antibody Purification (Protein A Column)

    Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.

    Publish: 2010/2/8

  • Antibody Conjugation - Biotinylation

    Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. A biotin/streptavidin (avidin) system offers low background, enhanced sensitivity and the ability to detect minute amounts of antigens.

    Publish: 2010/4/26

  • The Advantages of Chicken Antibody

    The advantages on using chicken antibody are well discussed in this AbVideo. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to the advatages of chicken antibody.

    Publish: 2014/7/8

  • Antibody Conjugation - FITC

    The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability.

    Publish: 2016/11/17

  • Antibody Purification (Affinity)

    Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix.

    Publish: 2010/4/26

  • Emulsification (CFA and IFA)

    Emulsification is a process to mix adjuvants and immunogen. Adjuvants are mixed and injected with an antigen to prevent catabolism and help increase the immune response by localizing the antigen for an extended time and attracting the appropriate cells (T cells, B cells and APC) to interact with it.

    Publish: 2010/8/26

  • Chicken Immunization

    Immunization can be a tricky process. This episode on the chicken antibody talks about the variables important for chicken antibody production. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to the immunization of the chicken.

    Publish: 2014/7/22

  • Chicken Antibody Introduction

    First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than an alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, for it is much more hygienic and cost efficient as well the manufacturing process is more humane. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here is a brief introduction for your first acquaintance of chicken antibody.

    Publish: 2014/7/1

  • Chicken Antibody Extraction

    This episode on chicken antibody describes the antibody extraction process. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to Chicken Antibody Extraction.

    Publish: 2014/7/29

  • Chicken Immune System

    This AbVideo provides in-depth knowledge on the immune system in chickens. First identified in 1969, avian IgY has now become a well characterized research reagent commercially available. For decades, extensive research has been carried out on its biological function, physiochemical property, production and purification. More than a alternative to mammalian IgG, IgY possesses several advantages over polyclonal antibodies raised in mammals, such as a much more hygienic, cost efficient, and humane manufacturing process. Abnova now brings you our latest series of avian antibodies for your choice. Please visit the webpage "New Chicken Antibodies" for more information. Here we present a brief introduction to the chicken immune system.

    Publish: 2014/7/15

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