This video shows how to subculture hESCs by collagenase IV. hESCs subculture is used to maintain master stocks of undifferentiated hESCs and minimize cell damage.
The Sandwich ELISA measures the amount of analyte between capture antibody and detection antibody. The analyte needs to have two different epitope sites available for antibody binding.
There are several steps after turn on and before turn off the flow cytometer. These steps are important to ensure the accuracy and reliability of results and consistent instrument functioning.
This video shows a novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel. [Reference: Biomacromolecules. 2007 Sep;8(9):2746-52]
A syringe filter is a single-use filter cartridge. It can be used to remove particles from a sample. We’ll show you how to use a syringe filter.
SDS-PAGE is a method used to separate proteins according to their size. This video shows you how to prepare SDS-PAGE with two layers of gel, separating gel and stacking gel.
Sandwich ELISA is performed to determine the amount and serological class of antibodies made by an immunized animal or present in the serum of patients. The anti-immunoglobulin antibodies used have high specificity and sensitivity.
A serial dilution is a series of simple dilutions which amplifies the dilution factor quickly beginning with a small initial quantity of material. The dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.
The growing cultures of cells need to be subcultured (or passaged) every few days to avoid overcrowding. Another reason that you need to subculture is that you can have more cell samples for research use. This video describes how to subculture the suspension cells.
SDS-PAGE is a technique used to separate proteins according to their electrophoretic mobility. SDS gel electrophoresis of samples have identical charge per unit mass due to binding of SDS results in fractionation by size, visualize the separated proteins, or process further application.
This video demonstrates how to subculture hESCs by mechanical dissection. Typically, hESCs subculture is used to maintain master stocks of undifferentiated hESCs and minimize cell damage.
Safranin is a biological stain used for the detection of cartilage, mucin and mast cell granules. This video shows how to stain chondrogenesis with safranin.
Sonicator agitates particles in a sample by applying sound (usually ultrasound) energy. In the laboratory, it is usually applied using an ultrasonic bath or an ultrasonic probe.
The Southern blot is used to detect a specific DNA sequence in DNA samples. A general blotting procedure starts with the separation of digested DNA fragments. The DNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.
The growing cultures of cells need to be subcultured (or passaged) every few days to avoid overcrowding. Another reason that you need to subculture is that you can have more cell samples for research use. This video shows how to subculture the adherent cells.