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  • Publish: 2014/12/16
  • Time: 6:06
Usually caused by sporadic 17p11.2 deletion encompassing the retinoic acid-induced 1 (RAI1) gene or a mutation of RAI1, Smith-Magenis syndrome generally involves variable mental retardation, sleep disturbance, craniofacial and facial abnormalities, developmental delay, and behavioral problems. Here we present a brief introduction to the common knowledge of Smith-Magenis syndrome.
TOP 10
  1. Western Blot
  2. Real-time PCR
  3. Reverse Transcription PCR
  4. Immunohistochemistry
  5. Indirect ELISA
  6. Polymerase Chain Reaction
  7. H&E Staining
  8. Cell Culture (Attached Cell)
  9. Immunofluorescence
  10. Competitive ELISA
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  • Nuclear and Cytoplasmic Protein Extraction

    Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells.

    Publish: 2011/4/19

  • Northern Blot

    The northern blot is used to study gene expression by detection of RNA (or isolated mRNA). A general blotting procedure starts with the separation of RNA by electrophoresis. The RNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.

    Publish: 2011/1/21

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