Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells.
The northern blot is used to study gene expression by detection of RNA (or isolated mRNA). A general blotting procedure starts with the separation of RNA by electrophoresis. The RNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.