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  • Publish: 2014/11/18
  • Time: 4:28
DiGeorge syndrome, first described in 1968 by the pediatric endocrinologist Angelo DiGeorge1, is a syndrome caused by the deletion of a small piece of chromosome 22. It is one of the most common causes of mental retardation due to a genetic deletion syndrome2, and, next to Down syndrome, it is also the most common genetic cause of congenital heart disease. Here we present a brief introduction to the common knowledge of DiGeorge syndrome.
TOP 10
  1. Western Blot
  2. Real-time PCR
  3. Reverse Transcription PCR
  4. Immunohistochemistry
  5. Indirect ELISA
  6. Polymerase Chain Reaction
  7. H&E Staining
  8. Cell Culture (Attached Cell)
  9. Immunofluorescence
  10. Competitive ELISA
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  • Northern Blot

    The northern blot is used to study gene expression by detection of RNA (or isolated mRNA). A general blotting procedure starts with the separation of RNA by electrophoresis. The RNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.

    Publish: 2011/1/21

  • Nuclear and Cytoplasmic Protein Extraction

    Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells.

    Publish: 2011/4/19

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