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  • Publish: 2014/12/23
  • Time: 3:40
First described in 1961 by J. C. p. Williams, a cardiologist in Auckland, Williams-Beuren syndrome (WBS; MIM 194050) is a rare developmental disorder with an occurrence of 1/20000-1/50000 live births. One of the characteristic feature of Williams-Beuren syndrome is overfriendliness with social-loving characteristics. Here we present a brief introduction to the common knowledge of Williams-Beuren syndrome.
TOP 10
  1. Western Blot
  2. Real-time PCR
  3. Reverse Transcription PCR
  4. Immunohistochemistry
  5. Indirect ELISA
  6. Polymerase Chain Reaction
  7. H&E Staining
  8. Cell Culture (Attached Cell)
  9. Immunofluorescence
  10. Competitive ELISA
  • List alphabetically by video name :
  • Result: 1 - 20
  • Pages:  1  2 
   
  • Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)

    Mycoplasma is a common and serious contamination of cell cultures. Mycoplasma contamination can cause the alteration of the phenotypic cell characteristics. PCR method allows for fast and reliable identification of mycoplasma contamination in cell cultures.

    Publish: 2013/4/8

  • Micro-Manipulator - Probe Preparation

    With the aid of micromanipulator, precise physical interactions under microscopic level becomes achievable. In concert with hydraulic drive systems, it is principally the glass probe that makes the physical contack with target cell for purposes like single cell collection, injection, patch clamping, etc. Here is the presentation of a typical procedure for probe preparation, including steps of glass capillary pulling and micropipette tip processing. Instruments adopted here are MF-900 microforge and PC-10 puller, both from NARISHIGE Group.

    Publish: 2014/4/15

  • mRNA Extraction

    Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

    Publish: 2010/4/19

  • Mononuclear Cell Isolation from Whole Blood

    This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps.

    Publish: 2010/5/31

  • MaxBead Carboxyl Labeling Kit Operation

    MaxBead Carboxyl Labeling Kit provides whole set of materials for researchers to bind the desired ligand onto magnetic beads conveniently. If only the ligand contain primary amine groups such as antibody, protein, or peptide, it can be conjugated to the magnetic beads easily.

    Publish: 2014/5/13

  • Migration Assay

    The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted.

    Publish: 2010/11/4

  • Making a Glass Knife

    This video shows you how to make a glass knife with capillary or pasteur pipette.

    Publish: 2011/8/2

  • MTT Assay (by BCRC)

    MTT assay allows assessing the viability and the proliferation of cells. This is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase.

    Publish: 2011/8/2

  • Mycoplasma Detection: Direct Method

    One of the most important cell culture procedures is using microtest to determine if mycoplasmal contaminant is present. This video demonstrates the steps of direct method for detection of Mycoplasma in the cell samples.

    Publish: 2011/8/8

  • MaxBead Protein A/G Operation

    MaxBead Protein A/G is designed as a rapid and simple tool for immunoprecipitation, purification/depletion assays, and other magnetic separation applications. Antibody can easily bind to the magnetic beads due to its high affinity with protein A/G.

    Publish: 2014/5/20

  • Miller-Dieker Syndrome

    Miller-Dieker syndrome is an autosomal dominant congenital disorder characterized by lissencephaly (smooth brain), which is resulted from incomplete neuronal migration. The disorder arises from the deletion of part of the small arm of chromosome 17p (which includes both the LIS1 and 14-3-3 epsilon gene), leading to partial monosomy. Here we present a brief introduction to the common knowledge of Miller-Dieker syndrome.

    Publish: 2014/12/2

  • Micropipette Guideline

    Pipetman is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation. This video provides some pipetting guidelines and precautions.

    Publish: 2010/10/21

  • MTT Assay

    MTT assay allows assessing the viability and the proliferation of cells. This is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase.

    Publish: 2010/8/26

  • Microwestern Arrays - Lecture Series 3

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 4

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 1

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Microwestern Arrays - Lecture Series 2

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Micro-Manipulator - Single Cell Collection

    With the aid of micromanipulator, precise physical interactions, like single cell collection, injection, patch clamping, etc. under microscopic level becomes achievable. In general, depending on the application, one or more micromanipulators can be docked on a microscope stage to operate in microscopes field of view. Here we present a typical procedure for single cell collection, including steps of cell aspiration and discharge. Instruments adopted here is MMO-203 Three-axis Oil Hydraulic Micromanipulator from NARISHIGE Group.

    Publish: 2014/4/22

  • Methylation-specific PCR

    Methylation-specific PCR (MSP) is a rapid and inexpensive method that can be used to determine the methylation status of DNA. It is a bisulfite conversion based PCR technique. In MSP, two primer pairs are designed for methylated and unmethylated DNA.

    Publish: 2010/8/5

  • Mouse Genotyping

    Genotyping is the process of determining the genes (genotype) of an individual by examining its DNA with the use of biological assays (ex: PCR, DNA sequencing). Three steps to type the mouse genotype : (i) isolate the genomic DNA from mouse tails; (ii) PCR to amplify with specific primers; (iii) electrophoresis to determine the genotype.

    Publish: 2010/8/26

  • Result: 1 - 20
  • Pages:  1  2 
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