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  • Publish: 2014/4/22
  • Time: 2:01
With the aid of micromanipulator, precise physical interactions, like single cell collection, injection, patch clamping, etc. under microscopic level becomes achievable. In general, depending on the application, one or more micromanipulators can be docked on a microscope stage to operate in microscopes field of view. Here we present a typical procedure for single cell collection, including steps of cell aspiration and discharge. Instruments adopted here is MMO-203 Three-axis Oil Hydraulic Micromanipulator from NARISHIGE Group.
TOP 10
  1. Western Blot
  2. Real-time PCR
  3. Reverse Transcription PCR
  4. Immunohistochemistry
  5. Indirect ELISA
  6. Polymerase Chain Reaction
  7. H&E Staining
  8. Cell Culture (Attached Cell)
  9. Immunofluorescence
  10. Competitive ELISA
  • List alphabetically by video name :
  • Result: 1 - 20
  • Pages:  1  2 
   
  • mRNA Extraction

    Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

    Publish: 2010/4/19

  • Micropipette Calibration

    Micropipettes are used to accurately measure and dispense small volumes of liquid. The capacity of a micropipette can range from less than 1μl to 1000μl (1ml). To ensure that obtained results using micropipette are reliable, it is necessary to calibrate micropipettes several times a year. This video shows a method for testing the accuracy of automatic micropipettes.

    Publish: 2010/7/19

  • Mycoplasma Detection: Direct Method

    One of the most important cell culture procedures is using microtest to determine if mycoplasmal contaminant is present. This video demonstrates the steps of direct method for detection of Mycoplasma in the cell samples.

    Publish: 2011/8/8

  • Mycoplasma Assay

    Mycoplasma is highly infectious and cross-contamination commonly occurs when new cells are introduced into laboratories. We use PCR-based method for detection of Mycoplasma weekly. It is a highly sensitive, specific and rapid method to detect mycoplasma contamination in cell cultures.

    Publish: 2010/3/22

  • Mycoplasma Detection: Hoechst DNA Staining

    One of the most important cell culture procedures is using microtest to determine if mycoplasmal contaminant is present. This video demonstrates the steps of Hoechst DNA staining method for detection of Mycoplasma in vero cell samples.

    Publish: 2011/8/8

  • MTT Assay

    MTT assay allows assessing the viability and the proliferation of cells. This is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase.

    Publish: 2010/8/26

  • Microwestern Arrays - Lecture Series 4

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Making a Glass Knife

    This video shows you how to make a glass knife with capillary or pasteur pipette.

    Publish: 2011/8/2

  • Microwestern Arrays - Lecture Series 3

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)

    Mycoplasma is a common and serious contamination of cell cultures. Mycoplasma contamination can cause the alteration of the phenotypic cell characteristics. PCR method allows for fast and reliable identification of mycoplasma contamination in cell cultures.

    Publish: 2013/4/8

  • Micropipette Guideline

    Pipetman is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation. This video provides some pipetting guidelines and precautions.

    Publish: 2010/10/21

  • Mycoplasma Detection Agar Preparation

    Mycoplasma detection agar (without crystal violet) is used with enrichments for the identification and cultivation of Mycoplasma. This video demonstrates the steps to prepare Mycoplasma agar.

    Publish: 2011/12/2

  • Micro-Manipulator - Single Cell Collection

    With the aid of micromanipulator, precise physical interactions, like single cell collection, injection, patch clamping, etc. under microscopic level becomes achievable. In general, depending on the application, one or more micromanipulators can be docked on a microscope stage to operate in microscopes field of view. Here we present a typical procedure for single cell collection, including steps of cell aspiration and discharge. Instruments adopted here is MMO-203 Three-axis Oil Hydraulic Micromanipulator from NARISHIGE Group.

    Publish: 2014/4/22

  • MTT Assay (by BCRC)

    MTT assay allows assessing the viability and the proliferation of cells. This is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase.

    Publish: 2011/8/2

  • Microwestern Arrays - Lecture Series 1

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Migration Assay

    The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted.

    Publish: 2010/11/4

  • Micro-Manipulator - Probe Preparation

    With the aid of micromanipulator, precise physical interactions under microscopic level becomes achievable. In concert with hydraulic drive systems, it is principally the glass probe that makes the physical contack with target cell for purposes like single cell collection, injection, patch clamping, etc. Here is the presentation of a typical procedure for probe preparation, including steps of glass capillary pulling and micropipette tip processing. Instruments adopted here are MF-900 microforge and PC-10 puller, both from NARISHIGE Group.

    Publish: 2014/4/15

  • Mouse Genotyping

    Genotyping is the process of determining the genes (genotype) of an individual by examining its DNA with the use of biological assays (ex: PCR, DNA sequencing). Three steps to type the mouse genotype : (i) isolate the genomic DNA from mouse tails; (ii) PCR to amplify with specific primers; (iii) electrophoresis to determine the genotype.

    Publish: 2010/8/26

  • Microwestern Arrays - Lecture Series 2

    Conference: 8th Bioinformatics and Systems Biology in Taiwan (BIT) Systems analysis of signaling pathways in human cancer cells with high-throughput western blotting assay-the microwestern arrays (Dr. Chih-Pin Chuu).

    Dr. Chuu developed the Microwestern arrays, a high throughput western blotting assay, which enables quantitative, sensitive and high throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates.

    Publish: 2010/10/7

  • Mononuclear Cell Isolation from Whole Blood

    This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps.

    Publish: 2010/5/31

  • Result: 1 - 20
  • Pages:  1  2 
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