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  • Publish: 2014/12/16
  • Time: 6:06
Usually caused by sporadic 17p11.2 deletion encompassing the retinoic acid-induced 1 (RAI1) gene or a mutation of RAI1, Smith-Magenis syndrome generally involves variable mental retardation, sleep disturbance, craniofacial and facial abnormalities, developmental delay, and behavioral problems. Here we present a brief introduction to the common knowledge of Smith-Magenis syndrome.
TOP 10
  1. Western Blot
  2. Real-time PCR
  3. Reverse Transcription PCR
  4. Immunohistochemistry
  5. Indirect ELISA
  6. Polymerase Chain Reaction
  7. H&E Staining
  8. Cell Culture (Attached Cell)
  9. Immunofluorescence
  10. Competitive ELISA
  • List alphabetically by video name :
  • Result: 1 - 11
  • Pages:  1 
  • Immunofluorescence

    Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope.

    Publish: 2009/12/13

  • Immunohistochemistry

    Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.

    Publish: 2009/12/7

  • Insect Cell Culture

    The Sf9 cell line derived from pupal ovarian tissue of the Fall armyworm Spodoptera frugiperda is one of the most common cell lines used for BEVS (Baculovirus expression vector systems). This video shows you how to culture the Sf9 cell line.

    Publish: 2010/5/31

  • Integra CL350 Cell Cultivation

    The CELLine bioreactor is a disposable, two-compartment cultivation device suitable for many cell culture applications. There are two sizes of the CELLine, CL350 and CL 1000. We use CL350 to produce monoclonal antibodies on a laboratory scale.

    Publish: 2010/6/30

  • Inactivated Feeder Cells (MEF) Thawing

    Mouse embryonic fibroblasts (MEFs) have been used as feeder cell layers for the culture of Human Embryonic Stem (hES) cells. MEFs could promote optimum growth and prevent differentiation of hESCs. This video describes how to thaw MEF cells and seed in plate for usage.

    Publish: 2011/12/2

  • Indirect ELISA

    The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies.

    Publish: 2010/4/26

  • Isolation of cccDNA

    cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.

    Publish: 2011/6/29

  • Isotope Labeling

    Isotopic labeling is a technique for labeling a substance with a stable or radioactive isotope. By measuring the radioactivity or the abundance of the isotope, we could made observations of the course through a biologic process. This video shows the procedure for using radioisotope phosphorus-32 to label DNA.

    Publish: 2011/7/1

  • Immunoprecipitation

    Immunoprecipitation (IP) is the technique of precipitating an protein antigen out of solution using an antibody specific to that antigen. This antibody is immobilized on a solid-phase substrate such as protein A/G agarose beads. The beads are then added to the protein mixture and the proteins that are targeted by the antibodies are captured onto the beads.

    Publish: 2011/4/19

  • Invasion Assay

    Cell invasion assay is designed to accelerate the screening process for compounds that influence cell migration through extracellular matrices. Matrigel (BD), extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, resembles the extracellular matrices is used by cell biologists as a substrate in cell invasion assay.

    Publish: 2010/11/12

  • IHC Kit - RealBlue Peroxidase Substrate Kit

    RealBlue Peroxidase Substrate kit is 50-100 times more sensitive than DAB and is non-carcinogenic. Strongly recommended for the detection of least-abundant markers. RealBlue forms a blue chromogenic product, providing excellent contrast with DAB and other substrates for multiple labeling experiments

    Publish: 2010/12/10

  • Result: 1 - 11
  • Pages:  1 
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