The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
The detection of bacteria and fungus is used as part of a routine and regular quality control screening procedure in cell culture. These contaminations are generally visible to the naked eye and detected by a sudden increase in turbidity and color change of the culture medium as the result of a change in pH.
BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media. This video shows you the steps of coating procedure of BD Matrigel.
Primer-BLAST was developed to help users make primers that are specific to the input PCR template. It uses Primer3 to design PCR primers and then submits them to BLAST search against user-selected database. The blast results are then automatically analyzed to avoid primer pairs that can cause amplification of targets other than the input template.
BLAST-Protein (blastp) is used for both identifying a query amino acid sequence and for finding similar sequences in protein databases. Like other BLAST programs, blastp is designed to find local regions of similarity. When sequence similarity spans the whole sequence, blastp will also report a global alignment, which is the preferred result for protein identification purposes.
BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media. This video shows you how to create aliquots of BD Matrigel when only small volumes are used at a time.
Multiple sequence alignment is an extension of pairwise alignment to incorporate more than two sequences at a time. It is often used to identify conserved sequence regions which are assumed to be evolutionarily related. By construct phylogenetic trees, it aids in establishing evolutionary relationships.
Biological Safety Cabinets (BSC) is an enclosed, ventilated laboratory workspace which provides the most effective primary containment for working with infectious agents. The BSC is tested for bioburden to ensure the adequate protection.
A blood smear gives information about the number and shape of blood cells. To perform a blood smear, two very clean slides are required. Drop a small drop of blood on one of the slides. Pull blood forward across slide.
The BioSpectrum® Imaging System is designed to automate the research with one-touch, pre-set or user-defined controls for accurate, repeatable imaging and analysis for chemiluminescent, bioluminescent, fluorescent, colorimetric imaging. The BioSpectrum® is faster than film for western blot imaging.
BAD (Bcl-2 antagonist of cell death) belongs to the pro-apoptotic BH3-only subfamily of Bcl- 2 proteins. Physiological activity of BAD is highly controlled by phosphorylation. To further analyze the regulation of BAD function, the authors (Polzien L, Baljuls A, Albrecht M, Hekman M, Rapp UR) investigated the role of recently identified phosphorylation sites on BAD-mediated apoptosis.
An X-ray film can detect both the radioactive and chemiluminescent signals from Western, Northern or Southern blot. It is placed against the blot membrane in a light-proof cassette. After the exposure for an appropriate time period, the film is developed by an auto-processor in a darkroom.