The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation) in tissue and cell samples prepared for microscopy. Each detected signal is visualized as an individual fluorescent dot, these signals can be quantified (counted) and assigned to a specific subcellular location based on microscopy images.
Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.
Alizarin Red is used to identify calcium deposits in tissue sections. Calcium forms an Alizarin Red S-calcium complex in a chelation process. This video describes the procedure of Alizarin Red S Staining for osteogenesis.
Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. A biotin/streptavidin (avidin) system offers low background, enhanced sensitivity and the ability to detect minute amounts of antigens.
Abnova offers a variety of arrays for protein expression profiling. These arrays are designed for researchers to study highly relevant proteins in the specific research fields like Angiogenesis, Apoptosis, Cancer Marker, Cell Cycle, Cytokine, Hematopoiesis, Hormone, Signal Transduction and Stem Cell.
There are physical, chemical, and biological indicators that can be used to ensure decontamination effectiveness of autoclaves. This video shows how to use stainless steel logger monitoring temperature of autoclave.
Alkaline phosphatase (AP) is a universal stem cell membrane marker. The undifferentiated state of hESCs is characterized by high level of expression of AP. This video shows the steps of AP stain.
There are physical, chemical, and biological indicators that can be used to ensure decontamination effectiveness of autoclaves. This video shows the procedure for quality control test using self-contained biological indicators, which contain G. stearothermophilus spores.
Angelman Syndrome is characterized by severe developmental delay, speech impairment, gait ataxia and/or tremulousness of thelimbs, and a unique behavioral phenotype that includes happy demeanor and excessive laughter. In principle, its resulted from the deregulation of UBE3A gene located on chromosome 15. Here we present a brief introduction to the common knowledge of Angelman Syndrome.
This is recommended to creat aliquots of serum when only small volumes are used at a time. This video shows you how to thaw the serum, aliquot into the 50ml tubes and refreeze.
Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy. This video shows you how to infect culture cells with adenoviruses. It is an important step before the production of adenoviral vector.
Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy. This video shows you how to infect culture cells with adenoviruses and product adenoviral vector.
Polyethylene Glycol (PEG) is a long chain polymer that has been approved by the Food and Drug Administration for human intravenous, oral and dermal applications. Covalent attachment of PEG (PEGylation) to proteins can reduce their immunogenicity, minimize proteolytic cleavage and increase their serum half-life. PEG has also been attached to small molecules and liposomes for more selective delivery. PEG-modification of superparamagnetic iron oxide and quantum dots can improve their biocompatibility and reduce non-specific uptake. This video shows the procedure of sandwich ELISA assay for anti-PEG antibody pair.
The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability.
Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix.
The ApoTox-Glo™ Triplex Assay combines three Promega assay chemistries to assess viability, cytotoxicity and apoptosis within a single assay well. The first part of the assay simultaneously measures two protease activities: GF-AFC is used to measure cell viability, and bis-AAF-R110 to cytotoxicity. The second part of the assay uses the Caspase-GloR to measure caspase activity.
alamarBlueR works as a cell viability and proliferation indicator through the conversion of resazurin to resorufin. Resazurin, a non-fluorescent indicator dye, is converted to highly red fluorescent resorufin via reduction reactions of metabolically active cells. The amount of fluorescence produced is proportional to the number of living cells.
Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the agarose gel.
Atomic force microscopy (AFM) is a very high-resolution type of scanning probe microscopy, with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit. The AFM is one of the foremost tools for imaging, measuring, and manipulating matter at the nanoscale. This video shows the AFM basic tutorial.