Alkaline phosphatase (AP) is a universal stem cell membrane marker. The undifferentiated state of hESCs is characterized by high level of expression of AP. This video shows the steps of AP stain.
This AbVideo shows you how to create aliquots of BD Matrigel when only small volumes are used at a time. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media.
BD Matrigel Coating plays a basic but crucial role for BD Matrigel matrix, and this video presents you the steps of its procedure. BD Matrigel matrix has been used extensively as a substrate for culturing human embryonic stem (hES) cells with various conditioned or defined media.
Embryoid Bodies (EB) Formation is an in vitro method to evaluate the pluripotency of the cells. EBs are formed in ultra low attach dishes that promote the aggregation of cells.
Gelatin solution is used to coat 6-well plates for culture of Mouse Embryonic Fibroblasts (MEFs). MEFs may be used as feeder cell layer to support the survival and growth of human embryonic stem cells (hESCs). This video shows the steps for preparation of gelatin solution and gelatin-coated plates.
This video describes how a vial of cryopreserved hESCs is thawed and seeded on a feeder layer of inactivated Mouse Embryonic Fibroblasts (MEFs). Human embryonic stem cells (hESCs) are pluripotent cells able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm.
This video shows how to subculture hESCs by collagenase IV. hESCs subculture is used to maintain master stocks of undifferentiated hESCs and minimize cell damage.
This video demonstrates how to subculture hESCs by mechanical dissection. Typically, hESCs subculture is used to maintain master stocks of undifferentiated hESCs and minimize cell damage.
Mouse embryonic fibroblasts (MEFs) have been used as feeder cell layers for the culture of Human Embryonic Stem (hES) cells. MEFs could promote optimum growth and prevent differentiation of hESCs. This video describes how to thaw MEF cells and seed in plate for usage.