CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound (MTS). The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at 490nm.
The Cell Proliferation Reagent WST-1 is a clear, slightly red, ready-to-use solution, containing WST-1, a tetrazolium salt. By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of 440 nm.