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AbVideo™ - PCR Cloning (II) - Digestion

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  • Publish: 2010/3/29
  • Time: 1:46

by Abnova  


Digestion is the process of cutting DNA molecules with restriction endonucleases. These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA. In PCR cloning, insert and vector are cutted by the same restriction endonucleases and then join together in the next step, ligation.


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