An X-ray film can detect both the radioactive and chemiluminescent signals from Western, Northern or Southern blot. It is placed against the blot membrane in a light-proof cassette. After the exposure for an appropriate time period, the film is developed by an auto-processor in a darkroom.
The northern blot is used to study gene expression by detection of RNA (or isolated mRNA). A general blotting procedure starts with the separation of RNA by electrophoresis. The RNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.
The Southern blot is used to detect a specific DNA sequence in DNA samples. A general blotting procedure starts with the separation of digested DNA fragments. The DNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe.
Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein.
Ponceau S is a rapid and reversible staining method for locating protein bands on Western blots producing reddish pink stained bands. The stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and can be completely removed from the protein bands by continued washing.
SNAP i.d.™ System is a fast and convenient method for the detection of immunoreactive proteins on western blots. The amount of time required for immunodetection is greatly reduced in 30 minutes with this unique vacuum-driven system.
There are several wash steps involved in western blot protocol. Abnova designed a western blot washer machine which can change the washing buffer automatically and shake by programs we setup.