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- CAR T Cell Therapy: Efficacy & Safety
- Chimeric Antigen Receptor T Cell Therapy
- Circulating Tumor Cell Isolation
- Circulating Tumor Cells
- T Cell Immunometabolism
Antibody
- Antibody Conjugation - Biotinylation
- Antibody Conjugation - FITC
- Antibody Pair for Proximity Ligation Assay
- Antibody Purification (Affinity)
- Antibody Purification (Protein A Column)
- Chicken Antibody Extraction
- Chicken Antibody Introduction
- Chicken Immune System
- Chicken Immunization
- Emulsification (CFA and IFA)
- The Advantages of Chicken Antibody
Beads
- MaxBead Carboxyl Labeling Kit Operation
- MaxBead Protein A/G Operation
Bioinformatics
- BLAST - Multiple Alignment
- BLAST - Nucleotide
- BLAST - PCR Primer Design
- BLAST - Protein
- Cytoscape
- EHCO - Encyclopedia of Hepatocellular Carcinoma Genes Online
- FASTA Formatted Sequence Record
- Gene Conserved Domains
- Gene Homology
- Gene Intron-Exon
- Gene Map Viewer
- Gene ORF Finder
- Gene Pathway
- Gene SNP
- GeneCards®
- GEO DataSets
- Human Protein Reference Database
- OMIM Database
- POINeT - Protein Interactome Datasets
- Protein Structure
- PubMed Literature Database
Calibration
- Autoclave Quality Control Test - Biological Indicator
- Autoclave Quality Control Test - Temperature Logger
- Micropipette Calibration
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Cell Assays
- alamarBlueR® Cell Proliferation Indicator
- Antibody Array for Protein Expression Profiling
- ApoTox-Glo™ Triplex Assay
- Cell Proliferation Assay (MTS)
- Cell Proliferation Assay (WST-1)
- Clonogenic Assay
- Invasion Assay
- Lactate Dehydrogenase Assay Kit
- Luciferase Assay
- Migration Assay
- MTT Assay
- MTT Assay (by BCRC)
- Raw Cell Transmigration
Cell Biology
- BAD in Cancer Signalling
- The Ciliopathy-associated Protein Homologs RPGRIP1
Cell Staining
- Alizarin Red S Staining for Osteogenesis
- Alkaline Phosphatase Staining
- Oil Red O Staining for Adipogenesis
- Propidium Iodide Staining
- Safranin Staining for Chondrogenesis
- Wright and Giemsa Staining
Clinical
- Blood Smear
- Down Syndrome
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- Patau Syndrome
- Prenatal Tests for Genetic Disorders
- Trisomy X Syndrome
- Urinalysis
- Warsaw Breakage Syndrome
- Williams-Beuren Syndrome
- Wolf-Hirschhorn Syndrome
CytoQuest™ CR
- CytoQuest™ CR Automated Leucosep Preparation
- CytoQuest™ CR Bubbles & Microfluidics - Dos and Don`ts
- CytoQuest™ CR Capture Antibody Immobilization
- CytoQuest™ CR Cell Capture
- CytoQuest™ CR Cell Immunostaining
- CytoQuest™ CR CTC Capture and CTC FISH - Dos and Don`ts
- CytoQuest™ CR CTC FISH
- CytoQuest™ CR Installation
- CytoQuest™ CR Leucosep Preparation
- CytoQuest™ CR Plugs Assembly and CytoChipNano Placement - Dos and Don`ts
- CytoQuest™ CR SCx™ Spiral Chamber Assembly - Dos and Don`ts
- CytoQuest™ CR System Maintenance
- CytoQuest™ CR Unpacking
CytoView™
- CytoView™ All-in-One Imaging System
DNA
- Agarose DNA Gel Preparation (by BCRC)
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- DNA Gel Preparation
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- Gateway® Recombination
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- PCR Purification
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- Plasmid DNA Extraction (Gigaprep)
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- Plasmid DNA Extraction (Miniprep)
- Plasmid DNA Extraction (Traditional Alkaline Lysis Method)
- Polymerase Chain Reaction
- Real-Time PCR
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Dye
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- Fluorescent Dye - Introduction
- Fluorescent Dye - Protein Labeling
ELISA
- Anti-PEG Antibody Pair
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- ELISA Substrate Kit - Introduction
- FSH ELISA Kit
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- Sandwich ELISA - Isotype Detection
- Total IgG (Human) ELISA Kit
Equipment
- Micropipette Guideline
- Pipette (Glass) Washing
- Sonicator
- Tissue Grinder
FISH
- Fluorescence In Situ Hybridization (FISH)
- mutaFISH™ - How It Works
- mutaFISH™ (Circulating Tumor Cell)
- mutaFISH™ (Frozen Tissue)
Flow Cytometry
- Flow Cytometry - Cell Cycle
- Flow Cytometry - Double Staining
- Flow Cytometry - Single Staining
- Start-up and Clean-up Procedures for Flow Cytometer
Immunofluorescence
- Immunofluorescence
Immunohistochemistry
- H&E Staining
- IHC Kit - RealBlue Peroxidase Substrate Kit
- Immunohistochemistry
- Rotary Microtome Section
- Tissue Embedding
- Tissue Microarray
Immunoprecipitation
- Immunoprecipitation
Instruments
- Atomic Force Microscopy(AFM) Basic Tutorial
- BioSpectrum® Imaging System
- Cell Counting - Cellometer®
- CO₂ Analyzer
- CO₂ Indicator
- Fluorescent Microscope
- Micro-Manipulator - Probe Preparation
- Micro-Manipulator - Single Cell Collection
- Precipitor™ 32
- SpiralPipet™
- UV-Vis Spectrophotometer - Nanodrop®
Isotope
- Isotope Labeling
- Radioactive Signal Detection
Lysates
- Cell Lysate Preparation (Denatured)
- Cell Lysate Preparation (Native)
- Cell Nuclear Protein Extraction
- Nuclear and Cytoplasmic Protein Extraction
- Tissue Lysate Preparation (Denatured)
- Tissue Lysate Preparation (Native)
- Transfected Lysate Preparation (Denatured)
- Transfected Lysate Preparation (Native)
Methods
- Blot Imaging with X-Ray Film
- Cell Fixation
- Cell Synchronization with Nocodazole at Mitosis
- Dialysis
- Dot Blot
- FastLink HRP Labeling Kit
- FastLink R-PE Labeling Kit
- Lyophilization
- Making a Glass Knife
- Mononuclear Cell Isolation from Whole Blood
- Mouse Genotyping
- Optical Fiber Cleavage
- Optical Fiber End-Face Analysis
- Serial Dilution
- Syringe Filter
- Volume Estimation in Pipette Tips
Microbial Culture
- Adenoviral Vector Production
- Adenovirus Infection
- Competent Cell Preparation
- Covalently Closed Circular DNA (cccDNA) Isolation
- Fluid Thioglycollate Medium Preparation
- LB Medium Preparation
- LB Plate Preparation
- Lentiviral Infection
- Lentiviral Production
- Lentivirus RIU Test
- Particle Blot for HBV Particle
- Plaque Assay for Influenza Virus
- Terrific Broth (TB) Preparation
- TSB Medium Preparation
mutaFISH™
- mutaFISH™ - How It Works
- mutaFISH™ (Circulating Tumor Cell)
- mutaFISH™ (Frozen Tissue)
Protein
- 2D Gel Electrophoresis (1) Protein Extraction
- 2D Gel Electrophoresis (10) Visible Staining
- 2D Gel Electrophoresis (11) Mass Sample Preparation
- 2D Gel Electrophoresis (12) Mass Analysis
- 2D Gel Electrophoresis (2) Protein Quantitation
- 2D Gel Electrophoresis (3) Sample Preparation for the First Dimension
- 2D Gel Electrophoresis (4) The First Dimension
- 2D Gel Electrophoresis (5) Gel Casting
- 2D Gel Electrophoresis (6) Reduction and Alkylation
- 2D Gel Electrophoresis (7) The Second Dimension
- 2D Gel Electrophoresis (8) Fluorescent Staining
- 2D Gel Electrophoresis (9) Image Capture - Laser Scanner
- 2D Gel Electrophoresis (9) Image Capture - UV Light Box
- Gelatin Zymography
- GST Enzymatic Digestion
- GST-tagged Protein Purification
- His-tagged Protein Purification
- Protein Quantification (BCA Assay)
- Protein Quantification (Bradford Assay)
- Protein Quantification (CBR)
- Protein Quantification (OD₂₈₀)
- Protein Quantification (Silver Staining)
- SDS-PAGE
- SDS-PAGE Gel Preparation
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 1
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 2
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 3
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 4
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 5
Protemist®
- Protemist® DT - Automated Wheat Germ Protein Synthesizer
- Protemist® DT II and XE - Demonstration
- Protemist® DT II and XE - Lecture Series
- Protemist® XE - Large-Scale Protein Expression
- Protemist® DT II - High Throughput Protein Expression
- Protemist® DT II - Operation
RNA
- Chimera RNA Interference
- DEPC Treatment
- mRNA Extraction
- Northern Blot
- Reverse Transcription PCR
- RNA Electrophoresis
- RNA Extraction by TRIzol®
- RNA Extraction from Cell Culture
- RNA Extraction from Tissue
- RNA Gel Preparation
Safety
- Bioburden Test of BSC
- Electrical Safety Test
- EtBr Handling
Tissue Culture
- Aliquoting and Preparation of Serum before Usage
- Bacteria/Fungus Detection
- Bacterial/Fungal Contaminant Detection
- BD Matrigel™ Aliquoting
- BD Matrigel™ Coating
- Calcium Phosphate Transfection
- Cationic Polymer Transfection
- Cell Counting
- Cell Counting (Attached Cells)
- Cell Counting (Cell Suspension)
- Cell Culture (Attached Cells)
- Cell Culture (Suspension Cell)
- Cell Culture Medium Preparation
- Cell Freezing Equipment - CoolCell®
- Cell Freezing Equipment - Mechanical Freezer
- Cell Freezing Equipment - Mr. Frosty™
- Cell Freezing Equipment - Programmable Freezer
- Cell Freezing Equipment - Styrofoam Box
- Cell Thawing
- Cell Thawing for DMSO-Sensitive Cells
- Chicken Myocyte Primary Culture
- Coated Plate Preparation
- Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)
- Electroporation
- Embryoid Body Formation - Ultra-Low Attachment Dishes
- Freezing of Adherent Cells
- Gelatin Preparation and Coating
- Handling Adherent Cells upon Receipt
- Handling Suspension Cells upon Receipt
- hESCs Cryopreservation
- hESCs Thawing
- Insect Cell Culture
- Integra CL350 Cell Cultivation
- Mycoplasma Assay
- Mycoplasma Detection - Direct Method
- Mycoplasma Detection - Hoechst DNA Staining
- Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)
- Mycoplasma Detection Agar Preparation
- Mycoplasma Detection Broth Preparation
- RNAi Transfection
- Stem Cell Embryoid Body Formation
- Subculture of Adherent Cells
- Subculture of hESCs with Collagenase IV
- Subculture of hESCs with Mechanical Dissection
- Subculture of Suspension Cells
- Thawing Inactivated Feeder Cells (MEFs)
Website
- Website Tutorial - Product Comparison
Western Blot
- Microwestern Arrays - Lecture Series 1
- Microwestern Arrays - Lecture Series 2
- Microwestern Arrays - Lecture Series 3
- Microwestern Arrays - Lecture Series 4
- Western Blot
- Western Blot - Ponceau S Staining
- Western Blot - SNAP id™ System
- Western Blot Membrane Stripping
- Western Blot Substrate Kit - Introduction
- Western Blot Washer

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AbVideo™ - Rotary Microtome Section

Video FAQ

Publish: 2017/1/24
Time: 1:40

by Abnova

Rotary microtome is an instrument used to cut the blocks of tissue embedded in paraffin into micro fine slices (known as sections). Microtome is an important device in microscopy preparation, allowing for the preparation of samples for observation under transmitted light or electron radiation.

Related Video

H&E Staining

H&E stain is a popular staining method in histology. Its a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you will usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink).

Publish: 2017/4/14
Immunohistochemistry

Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.

Publish: 2017/11/1