DNA electrophoresis is an analytical technique used to separate DNA fragments based on size, electrical charge and other physical properties. The DNA separates out into bands, with the distance from the electrode corresponding to length of the strand. The technique plays a role in identifying genes for diagnosing disease and for other forms of genetic research.
This AbVideo demonstrates step by step of the agarose gel preparation for DNA electrophoresis. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.
RNA electrophoresis is an analytical technique used to separate RNA fragments. RNA has the tendency to form both secondary and tertiary structures that can impede its separation. So a denaturing condition is performed in formaldehyde and MOPS buffer system. RNA under this condition is fully denatured and migrates according to its molecular weight.
SDS-PAGE is a technique used to separate proteins according to their electrophoretic mobility. SDS gel electrophoresis of samples have identical charge per unit mass due to binding of SDS results in fractionation by size, visualize the separated proteins, or process further application.