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- Antibody Purification (Protein A Column)
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- Chicken Antibody Introduction
- Chicken Immune System
- Chicken Immunization
- Emulsification (CFA and IFA)
- The Advantages of Chicken Antibody
Beads
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- MaxBead Protein A/G Operation
Bioinformatics
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Cell Biology
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- The Ciliopathy-associated Protein Homologs RPGRIP1
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CytoQuest™ CR
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CytoView™
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FISH
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Flow Cytometry
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- Start-up and Clean-up Procedures for Flow Cytometer
Immunofluorescence
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Immunoprecipitation
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Instruments
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- Precipitor™ 32
- SpiralPipet™
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Isotope
- Isotope Labeling
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Lysates
- Cell Lysate Preparation (Denatured)
- Cell Lysate Preparation (Native)
- Cell Nuclear Protein Extraction
- Nuclear and Cytoplasmic Protein Extraction
- Tissue Lysate Preparation (Denatured)
- Tissue Lysate Preparation (Native)
- Transfected Lysate Preparation (Denatured)
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Methods
- Blot Imaging with X-Ray Film
- Cell Fixation
- Cell Synchronization with Nocodazole at Mitosis
- Dialysis
- Dot Blot
- FastLink HRP Labeling Kit
- FastLink R-PE Labeling Kit
- Lyophilization
- Making a Glass Knife
- Mononuclear Cell Isolation from Whole Blood
- Mouse Genotyping
- Optical Fiber Cleavage
- Optical Fiber End-Face Analysis
- Serial Dilution
- Syringe Filter
- Volume Estimation in Pipette Tips
Microbial Culture
- Adenoviral Vector Production
- Adenovirus Infection
- Competent Cell Preparation
- Covalently Closed Circular DNA (cccDNA) Isolation
- Fluid Thioglycollate Medium Preparation
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- LB Plate Preparation
- Lentiviral Infection
- Lentiviral Production
- Lentivirus RIU Test
- Particle Blot for HBV Particle
- Plaque Assay for Influenza Virus
- Terrific Broth (TB) Preparation
- TSB Medium Preparation
mutaFISH™
- mutaFISH™ - How It Works
- mutaFISH™ (Circulating Tumor Cell)
- mutaFISH™ (Frozen Tissue)
Protein
- 2D Gel Electrophoresis (1) Protein Extraction
- 2D Gel Electrophoresis (10) Visible Staining
- 2D Gel Electrophoresis (11) Mass Sample Preparation
- 2D Gel Electrophoresis (12) Mass Analysis
- 2D Gel Electrophoresis (2) Protein Quantitation
- 2D Gel Electrophoresis (3) Sample Preparation for the First Dimension
- 2D Gel Electrophoresis (4) The First Dimension
- 2D Gel Electrophoresis (5) Gel Casting
- 2D Gel Electrophoresis (6) Reduction and Alkylation
- 2D Gel Electrophoresis (7) The Second Dimension
- 2D Gel Electrophoresis (8) Fluorescent Staining
- 2D Gel Electrophoresis (9) Image Capture - Laser Scanner
- 2D Gel Electrophoresis (9) Image Capture - UV Light Box
- Gelatin Zymography
- GST Enzymatic Digestion
- GST-tagged Protein Purification
- His-tagged Protein Purification
- Protein Quantification (BCA Assay)
- Protein Quantification (Bradford Assay)
- Protein Quantification (CBR)
- Protein Quantification (OD₂₈₀)
- Protein Quantification (Silver Staining)
- SDS-PAGE
- SDS-PAGE Gel Preparation
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 1
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 2
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 3
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 4
- Wheat Germ Cell-Free Protein Synthesis - Lecture Series 5
Protemist®
- Protemist® DT - Automated Wheat Germ Protein Synthesizer
- Protemist® DT II and XE - Demonstration
- Protemist® DT II and XE - Lecture Series
- Protemist® XE - Large-Scale Protein Expression
- Protemist® DT II - High Throughput Protein Expression
- Protemist® DT II - Operation
RNA
- Chimera RNA Interference
- DEPC Treatment
- mRNA Extraction
- Northern Blot
- Reverse Transcription PCR
- RNA Electrophoresis
- RNA Extraction by TRIzol®
- RNA Extraction from Cell Culture
- RNA Extraction from Tissue
- RNA Gel Preparation
Safety
- Bioburden Test of BSC
- Electrical Safety Test
- EtBr Handling
Tissue Culture
- Aliquoting and Preparation of Serum before Usage
- Bacteria/Fungus Detection
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- BD Matrigel™ Aliquoting
- BD Matrigel™ Coating
- Calcium Phosphate Transfection
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- Cell Culture (Suspension Cell)
- Cell Culture Medium Preparation
- Cell Freezing Equipment - CoolCell®
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- Cell Thawing
- Cell Thawing for DMSO-Sensitive Cells
- Chicken Myocyte Primary Culture
- Coated Plate Preparation
- Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)
- Electroporation
- Embryoid Body Formation - Ultra-Low Attachment Dishes
- Freezing of Adherent Cells
- Gelatin Preparation and Coating
- Handling Adherent Cells upon Receipt
- Handling Suspension Cells upon Receipt
- hESCs Cryopreservation
- hESCs Thawing
- Insect Cell Culture
- Integra CL350 Cell Cultivation
- Mycoplasma Assay
- Mycoplasma Detection - Direct Method
- Mycoplasma Detection - Hoechst DNA Staining
- Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)
- Mycoplasma Detection Agar Preparation
- Mycoplasma Detection Broth Preparation
- RNAi Transfection
- Stem Cell Embryoid Body Formation
- Subculture of Adherent Cells
- Subculture of hESCs with Collagenase IV
- Subculture of hESCs with Mechanical Dissection
- Subculture of Suspension Cells
- Thawing Inactivated Feeder Cells (MEFs)
Website
- Website Tutorial - Product Comparison
Western Blot
- Microwestern Arrays - Lecture Series 1
- Microwestern Arrays - Lecture Series 2
- Microwestern Arrays - Lecture Series 3
- Microwestern Arrays - Lecture Series 4
- Western Blot
- Western Blot - Ponceau S Staining
- Western Blot - SNAP id™ System
- Western Blot Membrane Stripping
- Western Blot Substrate Kit - Introduction
- Western Blot Washer

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AbVideo™ - Flow Cytometry - Single Staining

Video FAQ

Publish: 2011/8/2
Time: 1:38

by Abnova

This AbVideo exemplifies the procedure of single staining for flow cytometry analysis of cells. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus.

Related Video

Flow Cytometry - Cell Cycle

This AbVideo focuses on the flow cytometry analysis for a population of cell distribution at different cell cycle stages. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus.

Publish: 2011/8/2
Flow Cytometry - Double Staining

Step by step of double staining for flow cytometry analysis of cells is presented. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus.

Publish: 2017/12/1
Start-up and Clean-up Procedures for Flow Cytometer

There are several steps after turn on and before turn off the flow cytometer. These steps are important to ensure the accuracy and reliability of results and consistent instrument functioning.

Publish: 2011/8/2